BMRB Entry 18636

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PDB ID:
Entry in NMR Restraints Grid
Validation report in NRG-CING
Chem Shift validation: AVS_anomalous, AVS_full, LACS
BMRB Entry DOI: doi:10.13018/BMR18636
MolProbity Validation Chart

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Title:
1H, 13C and 15N resonance assignment of the soluble form of the Lipid-modified Azurin from Neisseria gonorrhoeae
Deposition date:
2012-08-03
Original release date:
2012-10-18
Authors:
Pauleta, Sofia; Matzapetakis, Manolis; Nobrega, Claudia
Citation:

Citation: Nobrega, Claudia; Matzapetakis, Manolis; Pauleta, Sofia. "1H, 13C and 15N resonance assignment of the soluble form of the Lipid-modified Azurin from Neisseria gonorrhoeae."  Biomol. NMR Assignments 7, 311-314 (2013).
PubMed: 23070845

Assembly members:

Assembly members:
Laz, polymer, 130 residues, 13630.2539 Da.

Natural source:

Natural source:   Common Name: b-proteobacteria   Taxonomy ID: 485   Superkingdom: Bacteria   Kingdom: not available   Genus/species: Neisseria gonorrhoeae

Experimental source:

Experimental source:   Production method: recombinant technology   Host organism: undefined   Vector: pET21c

Entity Sequences (FASTA):

Entity Sequences (FASTA):
Laz: ATAGNCAATVESNDNMQFNT KDIQVSKACKEFTITLKHTG TQPKASMGHNLVIAKAEDMD GVFKDGVGAADTDYVKPDDA RVVAHTKLIGGGEESSLTLD PAKLADGDYKFACTFPGHGA LMNGKVTLVD

Data sets:
Data typeCount
13C chemical shifts544
15N chemical shifts139
1H chemical shifts859

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
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Assembly:

Entity Assembly IDEntity NameEntity ID
1Laz1

Entities:

Entity 1, Laz 130 residues - 13630.2539 Da.

1   ALATHRALAGLYASNCYSALAALATHRVAL
2   GLUSERASNASPASNMETGLNPHEASNTHR
3   LYSASPILEGLNVALSERLYSALACYSLYS
4   GLUPHETHRILETHRLEULYSHISTHRGLY
5   THRGLNPROLYSALASERMETGLYHISASN
6   LEUVALILEALALYSALAGLUASPMETASP
7   GLYVALPHELYSASPGLYVALGLYALAALA
8   ASPTHRASPTYRVALLYSPROASPASPALA
9   ARGVALVALALAHISTHRLYSLEUILEGLY
10   GLYGLYGLUGLUSERSERLEUTHRLEUASP
11   PROALALYSLEUALAASPGLYASPTYRLYS
12   PHEALACYSTHRPHEPROGLYHISGLYALA
13   LEUMETASNGLYLYSVALTHRLEUVALASP

Samples:

13C15NLaz: Laz, [U-98% 13C; U-98% 15N], 1.5 mM; Phosphate 20.0 mM; Azide 1.0 mM; Ascorbate 5.0 mM; H2O 90%; D2O 10%

15NLaz: Laz, [U-98% 15N], 1.0 mM; Phosphate 20 mM; Azide 1 mM; Ascorbate 5 mM; H2O 90%; D2O 10%

1: ionic strength: 0.035 M; pH: 7.000; pressure: 1.000 atm; temperature: 298.000 K

Experiments:

NameSampleSample stateSample conditions
15N HSQC13C15NLazisotropic1
b_HNCO13C15NLazisotropic1
b_HNcaCo13C15NLazisotropic1
b_HNcoCACB13C15NLazisotropic1
b_HNCACB13C15NLazisotropic1
13C_HSQC13C15NLazisotropic1
hCCH_TOCSY13C15NLazisotropic1
HNHA15NLazisotropic1
13C HSQC NOESY13C15NLazisotropic1
15N HSQC NOESY13C15NLazisotropic1
J2 HSQC13C15NLazisotropic1

Software:

CARA v1.8, Keller and Wuthrich - Assignment

ANALYSIS v2.2, CCPN - data analysis

TOPSPIN v2.1, Bruker Biospin - Processing

NMR spectrometers:

  • Bruker Avance 800 MHz
  • Bruker Avance 600 MHz

Related Database Links:

PDB
EMBL CAA29561 CAA68589 CAM10669 CAX49737 CBA03903
GB AAF41888 AAW89678 ABX73607 ACF29476 ADO31997
PRF 1406258A
REF NP_274540 WP_002212858 WP_002219011 WP_002220564 WP_002222228
SP P07211 P07212 P57026
AlphaFold P07211 P07212 P57026

Download HSQC peak lists in one of the following formats:
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