BMRB Entry 18623

Click here to enlarge.

PDB ID:
Entry in NMR Restraints Grid
Validation report in NRG-CING
Chem Shift validation: AVS_anomalous, AVS_full, LACS
BMRB Entry DOI: doi:10.13018/BMR18623
MolProbity Validation Chart

NMR-STAR file interactive viewer.
NMR-STAR v3 text file.
XML gzip file.
RDF gzip file.
All files associated with the entry

Title:
Solution structure of mutant (T170E) second CARD of human RIG-I
Deposition date:
2012-07-27
Original release date:
2012-10-19
Authors:
Dutta, Kaushik
Citation:

Citation: Ferrage, Fabien; Dutta, Kaushik; Nistal-Villan, Estanislao; Patel, Jenish; Sanchez-Aparicio, Maria; De Ioannes, Pablo; Buku, Angeliki; Aseguinolaza, Gloria Gonzalez; Garcia-Sastre, Adolfo; Aggarwal, Aneel. "Structure and dynamics of the second CARD of human RIG-I provide mechanistic insights into regulation of RIG-I activation."  Structure 20, 2048-2061 (2012).
PubMed: 23063562

Assembly members:

Assembly members:
mutant (T170E) second CARD of human RIG-I, polymer, 100 residues, 11397.522 Da.

Natural source:

Natural source:   Common Name: Human   Taxonomy ID: 9606   Superkingdom: Eukaryota   Kingdom: Metazoa   Genus/species: Homo sapiens

Experimental source:

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli   Vector: pET15b

Entity Sequences (FASTA):

Entity Sequences (FASTA):
mutant (T170E) second CARD of human RIG-I: GSHMKKIEKLEEYRLLLKRL QPEFKTRIIPTDIISDLSEC LINQECEEILQICSTKGMMA GAEKLVECLLRSDKENWPKE LKLALEKERNKFSELWIVEK

Data sets:
Data typeCount
13C chemical shifts431
15N chemical shifts96
1H chemical shifts679

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1mutant (T170E) second CARD of human RIG-I1

Entities:

Entity 1, mutant (T170E) second CARD of human RIG-I 100 residues - 11397.522 Da.

1   GLYSERHISMETLYSLYSILEGLULYSLEU
2   GLUGLUTYRARGLEULEULEULYSARGLEU
3   GLNPROGLUPHELYSTHRARGILEILEPRO
4   THRASPILEILESERASPLEUSERGLUCYS
5   LEUILEASNGLNGLUCYSGLUGLUILELEU
6   GLNILECYSSERTHRLYSGLYMETMETALA
7   GLYALAGLULYSLEUVALGLUCYSLEULEU
8   ARGSERASPLYSGLUASNTRPPROLYSGLU
9   LEULYSLEUALALEUGLULYSGLUARGASN
10   LYSPHESERGLULEUTRPILEVALGLULYS

Samples:

sample_1: card2_mutant, [U-100% 15N], 300 uM; H2O 95%; D2O 5%; Bis-Tris 50 mM; sodium chloride 250 mM

sample_2: card2_mutant, [U-100% 13C; U-100% 15N], 300 uM; H2O 95%; D2O 5%; Bis-Tris 50 mM; sodium chloride 250 mM

sample_3: card2_mutant, [U-100% 13C; U-100% 15N], 300 uM; D2O 100%; Bis-Tris 50 mM; sodium chloride 250 mM

sample_conditions_1: ionic strength: 250 mM; pH: 7.5; pressure: 1 atm; temperature: 273 K

Experiments:

NameSampleSample stateSample conditions
2D 1H-15N HSQCsample_1isotropicsample_conditions_1
3D HNCOsample_2isotropicsample_conditions_1
3D HNCACBsample_2isotropicsample_conditions_1
3D CBCA(CO)NHsample_2isotropicsample_conditions_1
3D HBHA(CO)NHsample_2isotropicsample_conditions_1
3D C(CO)NHsample_2isotropicsample_conditions_1
3D H(CCO)NHsample_2isotropicsample_conditions_1
3D 1H-15N NOESYsample_2isotropicsample_conditions_1
3D 1H-13C NOESY aliphaticsample_3isotropicsample_conditions_1
3D 1H-13C NOESY aromaticsample_3isotropicsample_conditions_1

Software:

CNS, Brunger A. T. et.al. - refinement, structure solution

NMRView v8.3, Johnson, One Moon Scientific - chemical shift assignment

ARIA v2.3, Linge, O'Donoghue and Nilges - chemical shift assignment, chemical shift calculation

NMR spectrometers:

  • Bruker Avance 900 MHz
  • Bruker Avance 800 MHz
  • Bruker Avance 700 MHz

Related Database Links:

BMRB 18622
PDB
DBJ BAG11006 BAG54273
EMBL CAI46068
GB AAD19826 AAI07732 AAI32787 AAI36611 EAW58547
REF NP_055129 XP_001156662 XP_002819761 XP_003830148 XP_004047964
SP O95786
AlphaFold O95786

Download HSQC peak lists in one of the following formats:
CSV: Backbone or all simulated peaks
SPARKY: Backbone or all simulated peaks