BMRB Entry 18614

Name: NMR solution structure of Myo10 anti-CC
Published: 2012-09-24

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PDB ID: 2lw9
Entry in NMR Restraints Grid
Validation report in NRG-CING
Chem Shift validation: AVS_full
BMRB Entry DOI: doi:10.13018/BMR18614
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Bioinformatics (2015) 31 (8): 1322-1324 doi:10.1093/bioinformatics/btu829

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Title:

NMR solution structure of Myo10 anti-CC

Deposition date:

2012-07-25

Original release date:

2012-09-24

Authors:

Ye, Fei; Lu, Qing; Zhang, Mingjie

Citation:

Citation: Lu, Qing; Ye, Fei; Wei, Zhiyi; Wen, Zilong; Zhang, Mingjie. "Antiparallel coiled-coil-mediated dimerization of myosin X." Proc. Natl. Acad. Sci. U.S.A. 109, 17388-17393 (2012).
PubMed: 23012428

Assembly members:

Assembly members:
entity, polymer, 51 residues, 6339.12 Da.

Natural source:

Natural source: Common Name: E. coli Taxonomy ID: 562 Superkingdom: Bacteria Kingdom: not available Genus/species: Escherichia coli

Experimental source:

Experimental source: Production method: recombinant technology Host organism: Escherichia coli Vector: pET32

Entity Sequences (FASTA):

Entity Sequences (FASTA):
entity: ENKQVEEILRLEKEIEDLQR MKEQQELSLTEASLQKLQER RDQELRRLEEE

Data sets:

assigned_chemical_shifts
- assigned_chem_shift_list_1

Data type	Count
13C chemical shifts	155
15N chemical shifts	51
1H chemical shifts	329

Additional metadata:

Assembly
Samples and Experiments
Software
Spectrometers
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Assembly:

Entity Assembly ID	Entity Name	Entity ID
1	Myo10 anti-CC, 1	1
2	Myo10 anti-CC, 2	1

Entities:

Entity 1, Myo10 anti-CC, 1 51 residues - 6339.12 Da.

1

GLU

ASN

LYS

GLN

VAL

GLU

ILE

LEU

ARG

2

LEU

GLU

LYS

GLU

ILE

GLU

ASP

LEU

GLN

ARG

3

MET

LYS

GLU

GLN

GLU

LEU

SER

LEU

THR

4

GLU

ALA

SER

LEU

GLN

LYS

LEU

GLN

GLU

ARG

5

ARG

ASP

GLN

GLU

LEU

ARG

LEU

GLU

6

GLU

Samples:

sample_1: Myo10_anti-CC, [U-100% 13C; U-100% 15N], 0.8 mM; D2O 100%

sample_conditions_1: ionic strength: 100 mM; pH: 6.5; pressure: 1 atm; temperature: 303 K

Experiments:

Name	Sample	Sample state	Sample conditions
2D 1H-15N HSQC	sample_1	isotropic	sample_conditions_1
2D 1H-13C HSQC	sample_1	isotropic	sample_conditions_1
3D HNCACB	sample_1	isotropic	sample_conditions_1
3D CBCA(CO)NH	sample_1	isotropic	sample_conditions_1
3D 1H-15N NOESY	sample_1	isotropic	sample_conditions_1
3D 1H-13C NOESY	sample_1	isotropic	sample_conditions_1

Software:

CNS, Brunger, Adams, Clore, Gros, Nilges and Read - structure solution

NMRPipe, Delaglio, Grzesiek, Vuister, Zhu, Pfeifer and Bax - data analysis

SPARKY, Goddard - chemical shift assignment

PIPP, Garrett - chemical shift assignment

Molmol, Koradi, Billeter and Wuthrich - structure solution

NMR spectrometers:

Varian INOVA 800 MHz

PDB	2LW9
DBJ	BAA34519 BAG10382 BAH12170 BAH14509
EMBL	CAH10611
GB	AAF36524 AAF37875 AAF68025 AAI37169 AAI50286
REF	NP_036466 XP_001175408 XP_002745141 XP_002804360 XP_003263225
SP	Q9HD67
AlphaFold	Q9HD67