BMRB Entry 18612

Title:
NMR solution structure of human HisRS splice variant
Deposition date:
2012-07-24
Original release date:
2013-09-03
Authors:
Ye, Fei; Wei, Zhiyi; Wu, Jingjing; Schimmel, Paul; Zhang, Mingjie
Citation:

Citation: Ye, Fei; Wei, Zhiyi; Wu, Jingjing; Schimmel, Paul; Zhang, Mingjie. "NMR solution structure of human HisRS splice variant"  Structure ., .-..

Assembly members:

Assembly members:
HisRS, polymer, 170 residues, 19208.521 Da.

Natural source:

Natural source:   Common Name: Humans   Taxonomy ID: 9606   Superkingdom: Eukaryota   Kingdom: Metazoa   Genus/species: Homo sapiens

Experimental source:

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli   Vector: pET32

Data sets:
Data typeCount
13C chemical shifts503
15N chemical shifts162
1H chemical shifts1097

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1HisRS1

Entities:

Entity 1, HisRS 170 residues - 19208.521 Da.

1   ALAGLUARGALAALALEUGLUGLULEUVAL
2   LYSLEUGLNGLYGLUARGVALARGGLYLEU
3   LYSGLNGLNLYSALASERALAGLULEUILE
4   GLUGLUGLUVALALALYSLEULEULYSLEU
5   LYSALAGLNLEUGLYPROASPGLUSERLYS
6   GLNLYSPHEVALLEULYSTHRPROLYSALA
7   LEUGLUGLULYSILEARGTHRTHRGLUTHR
8   GLNVALLEUVALALASERALAGLNLYSLYS
9   LEULEUGLUGLUARGLEULYSLEUVALSER
10   GLULEUGLNASPALAGLYILELYSALAGLU
11   LEULEUTYRLYSLYSASNPROLYSLEULEU
12   ASNGLNLEUGLNTYRCYSGLUGLUALAGLY
13   ILEPROLEUVALALAILEILEGLYGLUGLN
14   GLULEULYSASPGLYVALILELYSLEUARG
15   SERVALTHRSERARGGLUGLUVALASPVAL
16   ARGARGGLUASPLEUVALGLUGLUILELYS
17   ARGARGTHRGLYGLNPROLEUSERILESER

Samples:

sample_1: entity, [U-100% 13C; U-100% 15N], 0.8 mM; D2O, nautral abundance, 100%

sample_conditions_1: ionic strength: 100 mM; pH: 6.5; pressure: 1 atm; temperature: 303 K

Experiments:

NameSampleSample stateSample conditions
2D 1H-15N HSQCsample_1isotropicsample_conditions_1
3D CBCA(CO)NHsample_1isotropicsample_conditions_1
3D HNCACBsample_1isotropicsample_conditions_1
2D 1H-1H NOESYsample_1isotropicsample_conditions_1
3D 1H-13C NOESYsample_1isotropicsample_conditions_1
3D 1H-15N NOESYsample_1isotropicsample_conditions_1

Software:

CNS, Brunger, Adams, Clore, Gros, Nilges and Read - structure solution

NMR spectrometers:

  • Varian INOVA 800 MHz

Related Database Links:

PDB
DBJ BAG54103
GB EAW62021

Download HSQC peak lists in one of the following formats:
CSV: Backbone or all simulated peaks
SPARKY: Backbone or all simulated peaks