BMRB Entry 18606

Title:
Solution structure of anti-CRISPR protein Acr30-35 from Pseudomonas aeruginosa Phage JBD30
Deposition date:
2012-07-22
Original release date:
2013-02-14
Authors:
Maxwell, Karen; Bondy-Denomy, Joe; Davidson, Alan
Citation:

Citation: Bondy-Denomy, Joe; Pawluk, April; Maxwell, Karen; Davidson, Alan. "Bacteriophage genes that inactivate the CRISPR/Cas bacterial immune system."  Nature 493, 429-432 (2013).
PubMed: 23242138

Assembly members:

Assembly members:
entity, polymer, 79 residues, 8825.006 Da.

Natural source:

Natural source:   Common Name: g-proteobacteria   Taxonomy ID: 1223260   Superkingdom: Bacteria   Kingdom: not available   Genus/species: Pseudomonas Pseudomonas Bacteriophage JBD30

Experimental source:

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli   Vector: pET15b-TVL

Entity Sequences (FASTA):

Data sets:
Data typeCount
13C chemical shifts239
15N chemical shifts75
1H chemical shifts509

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1anti-CRISPR protein Acr30-351

Entities:

Entity 1, anti-CRISPR protein Acr30-35 79 residues - 8825.006 Da.

1   GLYMETLYSPHEILELYSTYRLEUSERTHR
2   ALAHISLEUASNTYRMETASNILEALAVAL
3   TYRGLUASNGLYSERLYSILELYSALAARG
4   VALGLUASNVALVALASNGLYLYSSERVAL
5   GLYALAARGASPPHEASPSERTHRGLUGLN
6   LEUGLUSERTRPPHETYRGLYLEUPROGLY
7   SERGLYLEUGLYARGILEGLUASNALAMET
8   ASNGLUILESERARGARGGLUASNPRO

Samples:

sample_1: sodium phosphate 25 mM; sodium chloride 200 mM; H2O 90%; D2O 10%

sample_2: sodium phosphate 25 mM; sodium chloride 200 mM; D2O 100%

sample_conditions_1: ionic strength: 200 mM; pH: 6.8; pressure: 1 atm; temperature: 273 K

Experiments:

NameSampleSample stateSample conditions
3D 1H-15N NOESYsample_1isotropicsample_conditions_1
3D 1H-13C NOESYsample_1isotropicsample_conditions_1
2D 1H-13C HSQCsample_1isotropicsample_conditions_1
3D HNCOsample_1isotropicsample_conditions_1
3D HNCACBsample_1isotropicsample_conditions_1
3D CBCA(CO)NHsample_1isotropicsample_conditions_1
3D H(CCO)NHsample_1isotropicsample_conditions_1
3D C(CO)NHsample_1isotropicsample_conditions_1
2D 1H-15N HSQCsample_1isotropicsample_conditions_1
2D 1H-13C HSQCsample_1isotropicsample_conditions_1
3D 1H-13C NOESY aliphaticsample_2isotropicsample_conditions_1
3D 1H-13C NOESY aromaticsample_2isotropicsample_conditions_1
3D HCCH-TOCSYsample_2isotropicsample_conditions_1

Software:

TALOS, Cornilescu, Delaglio and Bax - data analysis

SPARKY, Goddard - chemical shift assignment, peak picking

NMR spectrometers:

  • Varian INOVA 500 MHz
  • Varian INOVA 800 MHz

Related Database Links:

PDB
DBJ BAR65419
EMBL CDM55208 CRN29922 CRN44629 CRN56640 CRN59456
GB AFE86461 AFQ21949 ERX38869 EZO98398
REF WP_016068276 WP_033975723 YP_006560529 YP_007392342

Download HSQC peak lists in one of the following formats:
CSV: Backbone or all simulated peaks
SPARKY: Backbone or all simulated peaks