BMRB Entry 18589

Name: NMR solution structure of Cbp2
Published: 2013-01-16

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PDB ID: 2lvs
Entry in NMR Restraints Grid
Validation report in NRG-CING
Chem Shift validation: AVS_full, LACS
BMRB Entry DOI: doi:10.13018/BMR18589
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Bioinformatics (2015) 31 (8): 1322-1324 doi:10.1093/bioinformatics/btu829

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Title:

NMR solution structure of Cbp2

Deposition date:

2012-07-11

Original release date:

2013-01-16

Authors:

Kenchappa, Chandra; Heidarsson, Petur; Garrett, Roger; Poulsen, Flemming

Citation:

Citation: Kenchappa, Chandra; Heidarsson, Petur; Kragelund, Birthe; Garrett, Roger; Poulsen, Flemming. "Solution properties of the archaeal CRISPR DNA repeat-binding homeodomain protein Cbp2." Nucleic Acids Res. 41, 3424-3435 (2013).
PubMed: 23325851

Assembly members:

Assembly members:
Cbp2, polymer, 105 residues, 12415 Da.

Natural source:

Natural source: Common Name: Hyperthermus butylicus Taxonomy ID: 54248 Superkingdom: Archaea Kingdom: not available Genus/species: Hyperthermus butylicus

Experimental source:

Experimental source: Production method: recombinant technology Host organism: Escherichia coli Vector: pET28a

Entity Sequences (FASTA):

Entity Sequences (FASTA):
Cbp2: MPSVNDSLDIVEKLYKDGVP VKEIAKRSNNSMSTVYKALE KLEAMGRIKRRKGRYRQHRR LTEEELATIRELYLKGATVY EIARQLGRPESTIYYALKKL GLKLE

Data sets:

assigned_chemical_shifts
- assigned_chem_shift_list_1

Data type	Count
13C chemical shifts	395
15N chemical shifts	89
1H chemical shifts	589

Additional metadata:

Assembly
Samples and Experiments
Software
Spectrometers
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Assembly:

Entity Assembly ID	Entity Name	Entity ID
1	Cbp2	1

Entities:

Entity 1, Cbp2 105 residues - 12415 Da.

1

MET

PRO

SER

VAL

ASN

ASP

SER

LEU

ASP

ILE

2

VAL

GLU

LYS

LEU

TYR

LYS

ASP

GLY

VAL

PRO

3

VAL

LYS

GLU

ILE

ALA

LYS

ARG

SER

ASN

4

SER

MET

SER

THR

VAL

TYR

LYS

ALA

LEU

GLU

5

LYS

LEU

GLU

ALA

MET

GLY

ARG

ILE

LYS

ARG

6

ARG

LYS

GLY

ARG

TYR

ARG

GLN

HIS

ARG

7

LEU

THR

GLU

LEU

ALA

THR

ILE

ARG

8

GLU

LEU

TYR

LEU

LYS

GLY

ALA

THR

VAL

TYR

9

GLU

ILE

ALA

ARG

GLN

LEU

GLY

ARG

PRO

GLU

10

SER

THR

ILE

TYR

ALA

LEU

LYS

LEU

11

GLY

LEU

LYS

LEU

GLU

Samples:

sample_1: Cbp2, [U-100% 13C; U-100% 15N], 0.5-1 mM; potassium chloride, none, 10 mM; potassium dihydrogen phosphate, none, 20 mM; sodium azide, none, 0.02%

sample_conditions_1: pH: 6.5; pressure: 1 atm; temperature: 298 K

Experiments:

Name	Sample	Sample state	Sample conditions
2D 1H-15N HSQC	sample_1	isotropic	sample_conditions_1
3D CBCA(CO)NH	sample_1	isotropic	sample_conditions_1
3D HNCO	sample_1	isotropic	sample_conditions_1
3D HNCA	sample_1	isotropic	sample_conditions_1
3D HNCACB	sample_1	isotropic	sample_conditions_1
3D HN(CO)CA	sample_1	isotropic	sample_conditions_1
3D HCCH-TOCSY	sample_1	isotropic	sample_conditions_1
3D 1H-15N NOESY	sample_1	isotropic	sample_conditions_1
3D 1H-13C NOESY	sample_1	isotropic	sample_conditions_1
3D HN(COCA)CB	sample_1	isotropic	sample_conditions_1

Software:

CYANA v2.1, Guntert, Mumenthaler and Wuthrich - structure solution

NMRPipe, Delaglio, Grzesiek, Vuister, Zhu, Pfeifer and Bax - processing

TALOS, Cornilescu, Delaglio and Bax - data analysis

VNMRJ, Varian - collection

CCPN_Analysis, CCPN - chemical shift assignment, peak picking

PSVS, Bhattacharya and Montelione - data analysis

NMR spectrometers:

Varian INOVA 750 MHz
Varian INOVA 800 MHz

PDB	2LVS
GB	ABM80833
REF	WP_011822151