BMRB Entry 18577

Chem Shift validation: AVS_full, LACS
BMRB Entry DOI: doi:10.13018/BMR18577

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Title:
Chemical Shift Assignments of the Connexin45 Carboxyl Terminal Domain: Dimer Conformation
Deposition date:
2012-07-06
Original release date:
2012-10-18
Authors:
Kopanic, Jennifer; Sorgen, Paul
Citation:

Citation: Kopanic, Jennifer; Sorgen, Paul. "Chemical shift assignments of the connexin45 carboxyl terminal domain: monomer and dimer conformations."  Biomol. NMR Assignments 7, 293-297 (2013).
PubMed: 23070843

Assembly members:

Assembly members:
Connexin45_Carboxyl_Terminal_Domain, polymer, 134 residues, 15005.5 Da.

Natural source:

Natural source:   Common Name: Rat   Taxonomy ID: 10116   Superkingdom: Eukaryota   Kingdom: Metazoa   Genus/species: Rattus norvegicus

Experimental source:

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli   Vector: pGEX-KT

Entity Sequences (FASTA):

Entity Sequences (FASTA):
Connexin45_Carboxyl_Terminal_Domain: GSKRRELDDPGAYNYPFTWN TPSAPPGYNIAVKPDQIQYT ELSNAKIAYKQNKANIAQEQ QYGSHEEHLPADLETLQREI RMAQERLDLAIQAYHHQNNP HGPREKKAKVGSKSGSNKSS ISSKSGDGKTSVWI

Data sets:
Data typeCount
13C chemical shifts406
15N chemical shifts92
1H chemical shifts631

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1Connexin45 Carboxyl Terminal Domain, chain 11
2Connexin45 Carboxyl Terminal Domain, chain 21

Entities:

Entity 1, Connexin45 Carboxyl Terminal Domain, chain 1 134 residues - 15005.5 Da.

1   GLYSERLYSARGARGGLULEUASPASPPRO
2   GLYALATYRASNTYRPROPHETHRTRPASN
3   THRPROSERALAPROPROGLYTYRASNILE
4   ALAVALLYSPROASPGLNILEGLNTYRTHR
5   GLULEUSERASNALALYSILEALATYRLYS
6   GLNASNLYSALAASNILEALAGLNGLUGLN
7   GLNTYRGLYSERHISGLUGLUHISLEUPRO
8   ALAASPLEUGLUTHRLEUGLNARGGLUILE
9   ARGMETALAGLNGLUARGLEUASPLEUALA
10   ILEGLNALATYRHISHISGLNASNASNPRO
11   HISGLYPROARGGLULYSLYSALALYSVAL
12   GLYSERLYSSERGLYSERASNLYSSERSER
13   ILESERSERLYSSERGLYASPGLYLYSTHR
14   SERVALTRPILE

Samples:

dimer_sample: Connexin45 Carboxyl Terminal Domain, [U-98% 13C; U-98% 15N], 0.9-1.2 mM; potassium chloride 2.7 mM; phosphate 11.9 mM; sodium chloride 137 mM; H20 90%; D20 10%

dimer_sample_conditions: ionic strength: 151.6 mM; pH: 5.8; pressure: 1 atm; temperature: 298 K

Experiments:

NameSampleSample stateSample conditions
2D 1H-15N HSQCdimer_sampleisotropicdimer_sample_conditions
2D 1H-13C HSQCdimer_sampleisotropicdimer_sample_conditions
3D HNCACBdimer_sampleisotropicdimer_sample_conditions
3D CBCA(CO)NHdimer_sampleisotropicdimer_sample_conditions
3D HNCOdimer_sampleisotropicdimer_sample_conditions
3D HN(CO)CAdimer_sampleisotropicdimer_sample_conditions
3D HCCH-TOCSYdimer_sampleisotropicdimer_sample_conditions
3D 1H-15N TOCSYdimer_sampleisotropicdimer_sample_conditions
3D 1H-15N NOESYdimer_sampleisotropicdimer_sample_conditions
3D 1H-13C NOESYdimer_sampleisotropicdimer_sample_conditions
3D C(CO)NHdimer_sampleisotropicdimer_sample_conditions
3D H(CCO)NHdimer_sampleisotropicdimer_sample_conditions

Software:

NMRView, Johnson, One Moon Scientific - chemical shift assignment

NMRPipe, Delaglio, Grzesiek, Vuister, Zhu, Pfeifer and Bax - processing

NMR spectrometers:

  • Varian INOVA 600 MHz

Related Database Links:

BMRB 18578
DBJ BAC34953
EMBL CAA44814 CAC21557
GB AAH50840 AAH71230 AAI66405 AAN17802 AAR98617
REF NP_001078850 NP_001152854 NP_001152855 NP_001233715 NP_001268477
SP A4GG66 P28229 Q6PYT3 Q6R4A8
AlphaFold A4GG66 P28229 Q6PYT3 Q6R4A8

Download HSQC peak lists in one of the following formats:
CSV: Backbone or all simulated peaks
SPARKY: Backbone or all simulated peaks