BMRB Entry 18548

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PDB ID:
Entry in NMR Restraints Grid
Validation report in NRG-CING
Chem Shift validation: AVS_full, LACS
BMRB Entry DOI: doi:10.13018/BMR18548
MolProbity Validation Chart

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Title:
1H, 13C, and 15N resonance assignments of the monomeric human Fam96a
Deposition date:
2012-06-25
Original release date:
2013-09-12
Authors:
Xia, Bin; Ouyang, Bingjie
Citation:

Citation: Ouyang, Bingjie; Wang, Lei; Wan, Shuo; Luo, Yang; Wang, Lu; Lin, Jian; Xia, Bin. "Solution structure of monomeric human FAM96A"  J. Biomol. NMR 56, 387-392 (2013).
PubMed: 23793605

Assembly members:

Assembly members:
Fam96a, polymer, 143 residues, Formula weight is not available

Natural source:

Natural source:   Common Name: Humans   Taxonomy ID: 9606   Superkingdom: Eukaryota   Kingdom: Metazoa   Genus/species: Homo sapiens

Experimental source:

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli   Vector: pPGH

Entity Sequences (FASTA):

Entity Sequences (FASTA):
Fam96a: MRQPRIMEEKALEVYDLIRT IRDPEKPNTLEELEVVSESC VEVQEINEEEYLVIIRFTPT VPHCSLATLIGLCLRVKLQR CLPFKHKLEIYISEGTHSTE EDINKQINDKERVAAAMENP NLREIVEQCVLEPDQLEHHH HHH

Data sets:
Data typeCount
13C chemical shifts595
15N chemical shifts136
1H chemical shifts1004

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1Fam96a1

Entities:

Entity 1, Fam96a 143 residues - Formula weight is not available

1   METARGGLNPROARGILEMETGLUGLULYS
2   ALALEUGLUVALTYRASPLEUILEARGTHR
3   ILEARGASPPROGLULYSPROASNTHRLEU
4   GLUGLULEUGLUVALVALSERGLUSERCYS
5   VALGLUVALGLNGLUILEASNGLUGLUGLU
6   TYRLEUVALILEILEARGPHETHRPROTHR
7   VALPROHISCYSSERLEUALATHRLEUILE
8   GLYLEUCYSLEUARGVALLYSLEUGLNARG
9   CYSLEUPROPHELYSHISLYSLEUGLUILE
10   TYRILESERGLUGLYTHRHISSERTHRGLU
11   GLUASPILEASNLYSGLNILEASNASPLYS
12   GLUARGVALALAALAALAMETGLUASNPRO
13   ASNLEUARGGLUILEVALGLUGLNCYSVAL
14   LEUGLUPROASPGLNLEUGLUHISHISHIS
15   HISHISHIS

Samples:

sample_1: Fam96a, [U-100% 13C; U-100% 15N], 0.5 mM; sodium phosphate 50 mM; sodium chloride 50 mM; EDTA 1 mM; DTT 50 mM; ARG 50 mM; GLU 50 mM; D2O 5%; H2O 95%

sample_conditions_1: ionic strength: 100 mM; pH: 7.0; pressure: 1 atm; temperature: 298 K

Experiments:

NameSampleSample stateSample conditions
2D 1H-15N HSQCsample_1isotropicsample_conditions_1
3D HNCACBsample_1isotropicsample_conditions_1
3D CBCA(CO)NHsample_1isotropicsample_conditions_1
3D HBHA(CO)NHsample_1isotropicsample_conditions_1
3D HNCOsample_1isotropicsample_conditions_1
3D HCCH-COSYsample_1isotropicsample_conditions_1
3D HCCH-TOCSYsample_1isotropicsample_conditions_1

Software:

NMRView, Johnson, One Moon Scientific - chemical shift assignment

NMRPipe, Delaglio, Grzesiek, Vuister, Zhu, Pfeifer and Bax - processing

NMR spectrometers:

  • Bruker Avance 700 MHz
  • Bruker Avance 600 MHz

Related Database Links:

BMRB 18137
PDB
DBJ BAB15496 BAB22285 BAC36966 BAE41803 BAG35105
GB AAH05745 AAH08865 AAI02257 AAI51429 ABQ82139
REF NP_001030282 NP_001092912 NP_080911 NP_115607 XP_002718208
SP Q3T0U7 Q9DCL2 Q9H5X1
TPG DAA25322
AlphaFold Q3T0U7 Q9DCL2 Q9H5X1

Download HSQC peak lists in one of the following formats:
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SPARKY: Backbone or all simulated peaks