BMRB Entry 18542

Name: Structure and Binding Interface of the Cytosolic Tails of aXb2 Integrin
Published: 2012-08-23

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PDB ID: 2luv
Entry in NMR Restraints Grid
Validation report in NRG-CING
Chem Shift validation: AVS_full, SPARTA
BMRB Entry DOI: doi:10.13018/BMR18542
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Bioinformatics (2015) 31 (8): 1322-1324 doi:10.1093/bioinformatics/btu829

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Title:

Structure and Binding Interface of the Cytosolic Tails of aXb2 Integrin

Deposition date:

2012-06-21

Original release date:

2012-08-23

Authors:

Tan, Suet Mien; Tang, Xiao Yan; Patra, Tanala

Citation:

Citation: Chua, Geok-Lin; Tang, Xiao-Yan; Patra, Alok Tanala; Tan, Suet-Mien; Bhattacharjya, Surajit. "Structure and binding interface of the cytosolic tails of X2 integrin." PLoS ONE 7, e41924-e41924 (2012).
PubMed: 22844534

Assembly members:

Assembly members:
entity, polymer, 35 residues, 3960.458 Da.

Natural source:

Natural source: Common Name: Human Taxonomy ID: 9606 Superkingdom: Eukaryota Kingdom: Metazoa Genus/species: Homo sapiens

Experimental source:

Experimental source: Production method: chemical synthesis

Entity Sequences (FASTA):

Entity Sequences (FASTA):
entity: KVGFFKRQYKEMMEEANGQI APENGTQTPSPPSEK

Data sets:

assigned_chemical_shifts
- assigned_chem_shift_list_1

Data type	Count
13C chemical shifts	28
1H chemical shifts	163

Additional metadata:

Assembly
Samples and Experiments
Software
Spectrometers
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Assembly:

Entity Assembly ID	Entity Name	Entity ID
1	Cytosolic Tails of aXb2 Integrin	1

Entities:

Entity 1, Cytosolic Tails of aXb2 Integrin 35 residues - 3960.458 Da.

1

LYS

VAL

GLY

PHE

LYS

ARG

GLN

TYR

LYS

2

GLU

MET

GLU

ALA

ASN

GLY

GLN

ILE

3

ALA

PRO

GLU

ASN

GLY

THR

GLN

THR

PRO

SER

4

PRO

SER

GLU

LYS

Samples:

MYR-ALPHA-X: DPC, [U-99% 2H], 200 mM; sodium phosphate 10 mM; D2O, [U-100% 2H], 10%; dodeclyphosphocholine 200 mM; H2O 90%; MYR-ALPHA-X 0.7 mM

MYR-ALPHA-X-D2O: DPC, [U-99% 2H], 200 mM; sodium phosphate 10 mM; D2O, [U-100% 2H], 100%; dodeclyphosphocholine 200 mM; MYR-ALPHA-X 0.7 mM

sample_conditions_1: ionic strength: 0 M; pH: 5.6; pressure: 1 atm; temperature: 308 K

Experiments:

Name	Sample	Sample state	Sample conditions
2D 1H-1H TOCSY	MYR-ALPHA-X	isotropic	sample_conditions_1
2D 1H-1H NOESY	MYR-ALPHA-X	isotropic	sample_conditions_1
2D 1H-13C HSQC	MYR-ALPHA-X-D2O	isotropic	sample_conditions_1
2D 1H-1H NOESY	MYR-ALPHA-X-D2O	isotropic	sample_conditions_1

Software:

TOPSPIN v2.1, Bruker Biospin - collection, processing

SPARKY, Goddard - chemical shift assignment

CYANA v2.0, Guntert, Mumenthaler and Wuthrich - structure solution

NMR spectrometers:

Bruker DRX 600 MHz

PDB	2LUV
DBJ	BAB84873
GB	AAA51620 AAA59180 AAH38237 AAX29959 ABM83412
REF	NP_000878 NP_001273304 XP_002826429 XP_003280513 XP_004057604
SP	P20702
AlphaFold	P20702