BMRB Entry 18537

Title:
1H and 13C chemical shifts for collagelin
Deposition date:
2012-06-20
Original release date:
2012-12-21
Authors:
Gnanagurunathan, Nimalta; Guenin, Erwann; Herve du Penhoat, Catherine
Citation:

Citation: Herve du Penhoat, Catherine; Gnanagurunathan, Nimalta; Guenin, Erwann. "Collagelin 1H and 13C NMR data"  .

Assembly members:

Assembly members:
collagelin, polymer, 23 residues, 2178.4 Da.

Natural source:

Natural source:   Common Name: not available   Taxonomy ID: not available   Superkingdom: not available   Kingdom: not available   Genus/species: not available not available

Experimental source:

Experimental source:   Production method: obtained from a vendor

Entity Sequences (FASTA):

Entity Sequences (FASTA):
collagelin: SGSGCGPRVMHGLHLGDDEG PCX

Data sets:
Data typeCount
13C chemical shifts41
1H chemical shifts97

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1collagelin1

Entities:

Entity 1, collagelin 23 residues - 2178.4 Da.

The C-termini residue is a cysteine with the carboxyl OH replaced by the NH2 moiety

1   SERGLYSERGLYCYSGLYPROARGVALMET
2   HISGLYLEUHISLEUGLYASPASPGLUGLY
3   PROCYSNH2

Samples:

sample_1: collagelin 3 mM; H2O 93%; D2O, [U-100% 2H], 7%; sodium phosphate 20 mM; sodium azide 5 uM; sodium chloride 50 mM

sample_conditions_1: ionic strength: 70 mM; pH: 5.4; pressure: 1 atm; temperature: 273 K

Experiments:

NameSampleSample stateSample conditions
2D 1H-13C HSQCsample_1isotropicsample_conditions_1
2D DQF-COSYsample_1isotropicsample_conditions_1
2D 1H-1H TOCSYsample_1isotropicsample_conditions_1
2D 1H-1H NOESYsample_1isotropicsample_conditions_1
2D 1H-1H TOCSYsample_1isotropicsample_conditions_1
2D 1H-1H NOESYsample_1isotropicsample_conditions_1

Software:

DYANA vversion 1.5, Bartels et al., Delaglio, Grzesiek, Vuister, Zhu, Pfeifer and Bax, Delaglio, Grzesiek, Vuister, Zhu, Pfeifer and Bax, Guntert, Guntert, Braun and Wuthrich, Koradi, Billeter and Wuthrich - chemical shift assignment, chemical shift assignment, peak picking, processing, refinement, structure solution

NMR spectrometers:

  • Varian UnityPlus 500 MHz