BMRB Entry 18527

Name: 1H chemical shifts for the C-terminal beta-tubulin 394-455 fragment
Published: 2012-07-13

Chem Shift validation: AVS_full
BMRB Entry DOI: doi:10.13018/BMR18527

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Nicholas Rego and David Koes
3Dmol.js: molecular visualization with WebGL
Bioinformatics (2015) 31 (8): 1322-1324 doi:10.1093/bioinformatics/btu829

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Title:

1H chemical shifts for the C-terminal beta-tubulin 394-455 fragment

Deposition date:

2012-06-16

Original release date:

2012-07-13

Authors:

Jimenez, M. Angeles; Andreu, Jose

Citation:

Citation: Andreu, M. Angeles; Evangelio, Juan; Aranda, Carlos; Lopez-Brauet, Adamari; Andreu, David; Rico, Manuel; Lagos, Rosalba; Andreu, Jose; Monasterio, Octavio. "Helicity of alpha(404-451) and bet(394-445) tubulin C-terminal recombinant peptides" Protein Sci. 8, 788-799 (1999).
PubMed: 10211825

Assembly members:

Assembly members:
beta_tubulin_394-445, polymer, 58 residues, Formula weight is not available

Natural source:

Natural source: Common Name: Humans Taxonomy ID: 9606 Superkingdom: Eukaryota Kingdom: Metazoa Genus/species: Homo sapiens

Experimental source:

Experimental source: Production method: recombinant technology Host organism: Escherichia coli Vector: pT7-7

Entity Sequences (FASTA):

Entity Sequences (FASTA):
beta_tubulin_394-445: ARIRAPFLHWYTGEGMDEME FTEAESNMNDLVSEYQQYQD ATADEQGEFEEEGEEDEA

Data sets:

assigned_chemical_shifts
- assigned_chem_shift_list_1

Data type	Count
1H chemical shifts	340

Additional metadata:

Assembly
Samples and Experiments
Software
Spectrometers
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Assembly:

Entity Assembly ID	Entity Name	Entity ID
1	beta tubulin 394-445	1

Entities:

Entity 1, beta tubulin 394-445 58 residues - Formula weight is not available

ARIRAP is the N-terminal cloning tag

1

ALA

ARG

ILE

ARG

ALA

PRO

PHE

LEU

HIS

TRP

2

TYR

THR

GLY

GLU

GLY

MET

ASP

GLU

MET

GLU

3

PHE

THR

GLU

ALA

GLU

SER

ASN

MET

ASN

ASP

4

LEU

VAL

SER

GLU

TYR

GLN

TYR

GLN

ASP

5

ALA

THR

ALA

ASP

GLU

GLN

GLY

GLU

PHE

GLU

6

GLU

GLY

GLU

ASP

GLU

ALA

Samples:

sample_1: beta tubulin 394-445 2 mM; H2O 63 v/v; D2O 7 v/v; TFE, [U-99% 2H], 30 v/v; TSP 0.2 mM

sample_conditions_1: ionic strength: 0 M; pH: 7.0; pressure: 1 atm; temperature: 298 K

Experiments:

Name	Sample	Sample state	Sample conditions
2D 1H-1H COSY	sample_1	isotropic	sample_conditions_1
2D 1H-1H TOCSY	sample_1	isotropic	sample_conditions_1
2D 1H-1H NOESY	sample_1	isotropic	sample_conditions_1

Software:

xwinnmr, Bruker Biospin - processing

NMR spectrometers:

Bruker AMX 600 MHz