BMRB Entry 18518

Assembly:

Entities:

Entity 1, entity_1 119 residues - 13965.232 Da.

Residues 1-4 are expression tag. Residues 5-119 correspond to residues 5-119 of human Microtubule-associated proteins 1A/1B light chain 3B (UniProtKB entry Q9GZQ8)

Entity 2, entity_2 17 residues - 2245.827 Da.

Residues 169-185 correspond to residues 169-185 of human Optineurin sequence (UniProtKB entry Q96CV9). All serines in the peptide are phosphoserines (SEP).

Samples:

sample_1: entity_1, [U-98% 13C; U-98% 15N], 0.6 ± 0.05 mM; entity_2 4.9 ± 0.5 mM; sodium phosphate 70 mM; sodium chloride 30 mM; DSS 0.3 mM; Protease inhibitors cocktail 5 mM; H2O 95%; D2O 5%

sample_2: entity_1, [U-98% 13C; U-98% 15N], 2.5 ± 0.05 mM; entity_2 0.4 ± 0.04 mM; sodium phosphate 70 mM; sodium chloride 30 mM; DSS 0.3 mM; Protease inhibitors cocktail 5 mM; H2O 95%; D2O 5%

sample_conditions_1: ionic strength: 0.05 M; pH: 6.8; pressure: 1 atm; temperature: 288 K

Experiments:

Software:

TOPSPIN v2, Bruker Biospin - chemical shift calculation, collection, processing

SPARKY v3.114, Goddard - chemical shift assignment, data analysis, peak picking

CYANA v2, Guntert, Mumenthaler and Wuthrich - structure solution

OPALp, Koradi, Billeter and Guntert - refinement

NMR spectrometers:

• Bruker Avance 900 MHz
• Bruker Avance 800 MHz
• Bruker Avance 700 MHz
• Bruker Avance 600 MHz
• Bruker Avance 500 MHz