BMRB Entry 18513
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PDB ID:
Entry in NMR Restraints Grid
Validation report in NRG-CING
Chem Shift validation: AVS_full, SPARTA
BMRB Entry DOI: doi:10.13018/BMR18513
MolProbity Validation Chart
NMR-STAR file interactive viewer.
NMR-STAR v3 text file.
XML gzip file.
RDF gzip file.
All files associated with the entry
Citation: Ramirez-Cordero, B.; Flores-Solis, D.; Saucedo, A.; Hernandez-Lupez, R.; Cano-Sanchez, P.; Chavez-Uribe, I.; del Rio-Portilla, F.. "Structural change due to deletion of two residues in GS-tamapin" to be published ., .-..
Assembly members:
recombinant-tamapin, polymer, 31 residues, 3470.188 Da.
Natural source: Common Name: Indian red scorpion Taxonomy ID: 34647 Superkingdom: Eukaryota Kingdom: Metazoa Genus/species: Mesobuthus tamulus
Experimental source: Production method: purified from natural source Host organism: Escherichia coli Vector: pET32a
Entity Sequences (FASTA):
recombinant-tamapin: AFCNLRRCELSCRSLGLLGK
CIGEECKCVPY
- assigned_chemical_shifts
| Data type | Count |
| 1H chemical shifts | 205 |
Additional metadata:
Assembly:
| Entity Assembly ID | Entity Name | Entity ID |
|---|---|---|
| 1 | recombinant-tamapin | 1 |
Entities:
Entity 1, recombinant-tamapin 31 residues - 3470.188 Da.
| 1 | ALA | PHE | CYS | ASN | LEU | ARG | ARG | CYS | GLU | LEU | ||||
| 2 | SER | CYS | ARG | SER | LEU | GLY | LEU | LEU | GLY | LYS | ||||
| 3 | CYS | ILE | GLY | GLU | GLU | CYS | LYS | CYS | VAL | PRO | ||||
| 4 | TYR |
Samples:
sample: recombinant-tamapin 0.8 mM; H2O 95%; D2O 5%
sample_conditions_1: pH: 6.0; pressure: 1 atm; temperature: 298 K
Experiments:
| Name | Sample | Sample state | Sample conditions |
|---|---|---|---|
| DQFCOSY | sample | isotropic | sample_conditions_1 |
| TOCSY | sample | isotropic | sample_conditions_1 |
| NOESY | sample | isotropic | sample_conditions_1 |
Software:
AMBER, Case, Darden, Cheatham, III, Simmerling, Wang, Duke, Luo, ... and Kollman - refinement
NMR spectrometers:
- Varian Inova 500 MHz