BMRB Entry 18505

Name: Backbone and side chain assignments of MHV N protein NTD (aa60-197)
Published: 2012-07-10

Chem Shift validation: AVS_full, LACS
BMRB Entry DOI: doi:10.13018/BMR18505

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Nicholas Rego and David Koes
3Dmol.js: molecular visualization with WebGL
Bioinformatics (2015) 31 (8): 1322-1324 doi:10.1093/bioinformatics/btu829

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Title:

Backbone and side chain assignments of MHV N protein NTD (aa60-197)

Deposition date:

2012-06-06

Original release date:

2012-07-10

Authors:

Keane, Sarah; Giedroc, David

Citation:

Citation: Keane, Sarah; Giedroc, David. "Backbone and side chain assignments of MHV N NTD" The BMRB entry is the only known published source for the data..

Assembly members:

Assembly members:
MHV_N_NTD, polymer, 138 residues, Formula weight is not available

Natural source:

Natural source: Common Name: Murine Hepatitis Virus Taxonomy ID: 11138 Superkingdom: Virus Kingdom: not available Genus/species: Betacoronavirus Murine coronavirus

Experimental source:

Experimental source: Production method: recombinant technology Host organism: Escherichia coli Vector: pMBP

Entity Sequences (FASTA):

Entity Sequences (FASTA):
MHV_N_NTD: GSVVPHYSWFSGITQFQKGK EFQFAEGQGVPIANGIPASE QKGYWYRHNRRSFKTPDGQQ KQLLPRWYFYYLGTGPHAGA SYGDSIEGVFWVANSQADTN TRSDIVERDPSSHEAIPTRF APGTVLPQGFYVEGSGRS

Data sets:

assigned_chemical_shifts
- assigned_chem_shift_list_1

Data type	Count
13C chemical shifts	422
15N chemical shifts	122
1H chemical shifts	694

Additional metadata:

Assembly
Samples and Experiments
Software
Spectrometers
Hide all

Assembly:

Entity Assembly ID	Entity Name	Entity ID
1	NTD	1

Entities:

Entity 1, NTD 138 residues - Formula weight is not available

1

GLY

SER

VAL

PRO

HIS

TYR

SER

TRP

PHE

2

SER

GLY

ILE

THR

GLN

PHE

GLN

LYS

GLY

LYS

3

GLU

PHE

GLN

PHE

ALA

GLU

GLY

GLN

GLY

VAL

4

PRO

ILE

ALA

ASN

GLY

ILE

PRO

ALA

SER

GLU

5

GLN

LYS

GLY

TYR

TRP

TYR

ARG

HIS

ASN

ARG

6

ARG

SER

PHE

LYS

THR

PRO

ASP

GLY

GLN

7

LYS

GLN

LEU

PRO

ARG

TRP

TYR

PHE

TYR

8

TYR

LEU

GLY

THR

GLY

PRO

HIS

ALA

GLY

ALA

9

SER

TYR

GLY

ASP

SER

ILE

GLU

GLY

VAL

PHE

10

TRP

VAL

ALA

ASN

SER

GLN

ALA

ASP

THR

ASN

11

THR

ARG

SER

ASP

ILE

VAL

GLU

ARG

ASP

PRO

12

SER

HIS

GLU

ALA

ILE

PRO

THR

ARG

PHE

13

ALA

PRO

GLY

THR

VAL

LEU

PRO

GLN

GLY

PHE

14

TYR

VAL

GLU

GLY

SER

GLY

ARG

SER

Samples:

sample_1: MHV N NTD, [U-100% 13C; U-100% 15N], 200 uM; H2O 90%; D2O 10%

sample_2: MHV N NTD, [U-100% 13C; U-100% 15N], 200 uM; D2O 100%

sample_conditions_1: ionic strength: 150 mM; pH: 6.0; pressure: 1 atm; temperature: 273 K

Experiments:

Name	Sample	Sample state	Sample conditions
2D 1H-15N HSQC	sample_1	isotropic	sample_conditions_1
3D HNCO	sample_1	isotropic	sample_conditions_1
3D CBCANH	sample_1	isotropic	sample_conditions_1
3D CBCA(CO)NH	sample_1	isotropic	sample_conditions_1
3D C(CO)NH	sample_1	isotropic	sample_conditions_1
3D H(CCO)NH	sample_1	isotropic	sample_conditions_1
3D HNHA	sample_1	isotropic	sample_conditions_1
3D 1H-15N TOCSY	sample_1	isotropic	sample_conditions_1
3D HNHB	sample_1	isotropic	sample_conditions_1
HBCBCGCDHD	sample_1	isotropic	sample_conditions_1
HBCBCGCDCEHE	sample_1	isotropic	sample_conditions_1
2D 1H-13C HSQC aliphatic	sample_2	isotropic	sample_conditions_1
2D 1H-13C HSQC aromatic	sample_2	isotropic	sample_conditions_1
3D HCCH-TOCSY	sample_2	isotropic	sample_conditions_1
3D HCCH-COSY	sample_2	isotropic	sample_conditions_1

Software:

SPARKY, Goddard - chemical shift assignment, data analysis, peak picking

NMRPipe, Delaglio, Grzesiek, Vuister, Zhu, Pfeifer and Bax - processing

NMR spectrometers:

Varian VNMRS 600 MHz
Varian VNMRS 800 MHz

PDB	3HD4
EMBL	CAA25198
GB	AAA46444 AAA46447 AAA46462 AAA74734 AAA76578
REF	NP_045302
SP	P03416 P18447 Q83360 Q9WCD0
AlphaFold	P03416 P18447 Q83360 Q9WCD0