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PDB ID:
Entry in NMR Restraints Grid
Validation report in NRG-CING
Chem Shift validation: AVS_full
BMRB Entry DOI: doi:10.13018/BMR18500
MolProbity Validation Chart
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Citation: Aragon, Eric; Goerner, Nina; Xi, Qiaoran; Gomes, Tiago; Gao, Sheng; Massague, Joan; Macias, Maria. "Structural Basis for the Versatile Interactions of Smad7 with Regulator WW Domains in TGF-beta Pathways" Structure 20, 1726-1736 (2012).
PubMed: 22921829
Assembly members:
SMURF1WW2, polymer, 35 residues, 4057.533 Da.
SMAD7, polymer, 15 residues, 1778.969 Da.
Natural source: Common Name: Humans Taxonomy ID: 9606 Superkingdom: Eukaryota Kingdom: Metazoa Genus/species: Homo sapiens
Experimental source: Production method: recombinant technology Host organism: Escherichia coli Vector: petM11
Entity Sequences (FASTA):
SMURF1WW2: GPLPPGWEVRSTVSGRIYFV
DHNNRTTQFTDPRLH
SMAD7: ELESPPPPYSRYPMD
Data type | Count |
1H chemical shifts | 281 |
Entity Assembly ID | Entity Name | Entity ID |
---|---|---|
1 | Smurf1 WW2 domain | 1 |
2 | Smad7 derived peptide | 2 |
Entity 1, Smurf1 WW2 domain 35 residues - 4057.533 Da.
1 | GLY | PRO | LEU | PRO | PRO | GLY | TRP | GLU | VAL | ARG | ||||
2 | SER | THR | VAL | SER | GLY | ARG | ILE | TYR | PHE | VAL | ||||
3 | ASP | HIS | ASN | ASN | ARG | THR | THR | GLN | PHE | THR | ||||
4 | ASP | PRO | ARG | LEU | HIS |
Entity 2, Smad7 derived peptide 15 residues - 1778.969 Da.
1 | GLU | LEU | GLU | SER | PRO | PRO | PRO | PRO | TYR | SER | ||||
2 | ARG | TYR | PRO | MET | ASP |
Homonuclear: SMURF1WW2 1 mM; SMAD7 2 mM; TRIS, [U-100% 2H], 20 mM; sodium chloride 100 mM; H2O 90%; D2O 10%
15N13C: SMURF1WW2, [U-100% 13C; U-100% 15N], 1 mM; SMAD7 3 mM; TRIS, [U-100% 2H], 20 mM; sodium chloride 100 mM; H2O 90%; D2O 10%
sample_conditions_1: pH: 7.2; pressure: 1 atm; temperature: 285 K
Name | Sample | Sample state | Sample conditions |
---|---|---|---|
2D 1H-15N HSQC | 15N13C | isotropic | sample_conditions_1 |
2D 1H-1H TOCSY | Homonuclear | isotropic | sample_conditions_1 |
2D 1H-1H NOESY | Homonuclear | isotropic | sample_conditions_1 |
3D CBCA(CO)NH | 15N13C | isotropic | sample_conditions_1 |
3D HNCACB | 15N13C | isotropic | sample_conditions_1 |
TOPSPIN, Bruker Biospin - collection
NMRPipe, Delaglio, Grzesiek, Vuister, Zhu, Pfeifer and Bax - processing
CARA, Keller and Wuthrich - chemical shift assignment, peak picking
CNSSOLVE, Brunger, Adams, Clore, Gros, Nilges and Read - structure solution
BMRB | 17543 16923 18501 18502 |
PDB | |
DBJ | BAB13451 BAB29770 BAE32623 BAG11347 BAG11040 |
EMBL | CAF92291 CAA04182 CAA04183 CAG12446 CDQ87305 CDQ93148 |
GB | AAC62434 AAF08298 AAH29097 AAI36805 AAI44415 AAB81246 AAB81353 AAB81354 AAC25062 AAD41130 |
REF | NP_001033716 NP_001103068 NP_001186776 NP_001244560 NP_065162 NP_001036125 NP_001153135 NP_001177750 NP_001231104 NP_001268265 |
SP | Q9CUN6 Q9HCE7 O15105 O35253 O88406 |
TPG | DAA15146 DAA15864 |
AlphaFold | Q9CUN6 Q9HCE7 O15105 O35253 O88406 |