BMRB Entry 18488

Chem Shift validation: AVS_full, LACS
BMRB Entry DOI: doi:10.13018/BMR18488

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Title:
Backbone 1H, 13C, and 15N Chemical Shift Assignments of an oxidizied and mutant form of sulfiredoxin from Saccharomyces cervisiae
Deposition date:
2012-05-29
Original release date:
2012-07-02
Authors:
Averlant-Petit, Marie-Christine; Beaufils, Chrystel; Rahuel-Clermont, Sophie; Branlant, Guy; Kriznik, Alexandre; Modh-Yahaya, Normi; Roussel, Xavier; Legrand, Baptiste
Citation:

Citation: Roussel, Xavier; Boukhenouna, Samia; Rahuel-Clermont, Sophie; Branlant, Guy. "The rate-limiting step of sulfiredoxin is associated with the transfer of the gamma-phosphate of ATP to the sulfinic acid of overoxidized typical 2-Cys peroxiredoxins"  FEBS Lett. 585, 574-578 (2011).
PubMed: 21237158

Assembly members:

Assembly members:
Oxydizied_Sulfiredoxin_C106V, polymer, 127 residues, Formula weight is not available

Natural source:

Natural source:   Common Name: baker's yeast   Taxonomy ID: 4932   Superkingdom: Eukaryota   Kingdom: Fungi   Genus/species: Saccharomyces cerevisiae

Experimental source:

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli   Vector: pET20bSrx C106V

Entity Sequences (FASTA):

Entity Sequences (FASTA):
Oxydizied_Sulfiredoxin_C106V: MSLQSNSVKPTEIPLSEIRR PLAPVLDPQKIDAMVATMKG IPTASKTCSLEQAEAAASAG ELPPVDVLGVRVKGQTLYYA FGGCHRLQAYDRRARETQNA AFPVRVRVLPATPRQIRMYL GSSLDIE

Data sets:
Data typeCount
13C chemical shifts435
15N chemical shifts97
1H chemical shifts549

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1Oxydizied Sulfiredoxin C106V1

Entities:

Entity 1, Oxydizied Sulfiredoxin C106V 127 residues - Formula weight is not available

1   METSERLEUGLNSERASNSERVALLYSPRO
2   THRGLUILEPROLEUSERGLUILEARGARG
3   PROLEUALAPROVALLEUASPPROGLNLYS
4   ILEASPALAMETVALALATHRMETLYSGLY
5   ILEPROTHRALASERLYSTHRCYSSERLEU
6   GLUGLNALAGLUALAALAALASERALAGLY
7   GLULEUPROPROVALASPVALLEUGLYVAL
8   ARGVALLYSGLYGLNTHRLEUTYRTYRALA
9   PHEGLYGLYCYSHISARGLEUGLNALATYR
10   ASPARGARGALAARGGLUTHRGLNASNALA
11   ALAPHEPROVALARGVALARGVALLEUPRO
12   ALATHRPROARGGLNILEARGMETTYRLEU
13   GLYSERSERLEUASPILEGLU

Samples:

sample_1: Oxydizied Sulfiredoxin C106V, [U-15N], 0.55 mM; H2O 95%; D2O 5%; NaPi 50 mM; NaCl 100 mM; DTT 50 mM

sample_2: Oxydizied Sulfiredoxin C106V, [U-13C; U-15N], 0.55 mM; H2O 95%; D2O 5%; NaPi 50 mM; NaCl 100 mM; DTT 50 mM

sample_3: Oxydizied Sulfiredoxin C106V, [U-13C; U-15N; U-2H], 0.55 mM; H2O 95%; D2O 5%; NaPi 50 mM; NaCl 100 mM; DTT 50 mM

sample_conditions_1: ionic strength: 150 mM; pH: 6; pressure: 1 atm; temperature: 298 K

Experiments:

NameSampleSample stateSample conditions
3D HNCOsample_1isotropicsample_conditions_1
3D HNCACBsample_1isotropicsample_conditions_1
3D CBCA(CO)NHsample_1isotropicsample_conditions_1
3D HCCH-TOCSYsample_1isotropicsample_conditions_1
3D 1H-15N TOCSYsample_1isotropicsample_conditions_1
3D 1H-15N NOESYsample_1isotropicsample_conditions_1
3D 1H-13C NOESY aliphaticsample_1isotropicsample_conditions_1

Software:

CARA, Keller and Wuthrich - chemical shift assignment

NMR spectrometers:

  • Bruker DRX 600 MHz
  • Bruker Avance 950 MHz

Related Database Links:

BMRB 18486
DBJ GAA24640
EMBL CAA81924 CAY81004
GB AAS56676 AHY76158 AJP39952 AJS30150 AJS30452
REF NP_012837
SP P36077
TPG DAA09072
AlphaFold P36077

Download HSQC peak lists in one of the following formats:
CSV: Backbone or all simulated peaks
SPARKY: Backbone or all simulated peaks