BMRB Entry 18463

Title:
Eurocin solution structure
Deposition date:
2012-05-15
Original release date:
2012-10-29
Authors:
Oeemig, Jesper; Lynggaard, Carina; Knudsen, Daniel; Hansen, Frederik; Noergaard, Kent; Schneider, Tanja; Vad, Brian; Neve, Soeren; Kristensen, Hans-Henrik; Sahl, Hans-Georg; Otzen, Daniel; Wimmer, Reinhard
Citation:

Citation: Oeemig, Jesper; Lynggaard, Carina; Knudsen, Daniel; Hansen, Frederik; Nrgaard, Kent; Schneider, Tanja; Vad, Brian; Sandvang, Dorthe; Nielsen, Line; Neve, Sren; Kristensen, Hans-Henrik; Sahl, Hans-Georg; Otzen, Daniel; Wimmer, Reinhard. "Eurocin, a new fungal defensin: structure, lipid binding, and its mode of action."  J. Biol. Chem. 287, 42361-42372 (2012).
PubMed: 23093408

Assembly members:

Assembly members:
Eurocin, polymer, 42 residues, 4348.852 Da.

Natural source:

Natural source:   Common Name: Ascomycetes   Taxonomy ID: 5054   Superkingdom: Eukaryota   Kingdom: Fungi   Genus/species: Eurotium amstelodami

Experimental source:

Experimental source:   Production method: recombinant technology   Host organism: Aspergillus oryzae   Vector: N/A

Entity Sequences (FASTA):

Entity Sequences (FASTA):
Eurocin: GFGCPGDAYQCSEHCRALGG GRTGGYCAGPWYLGHPTCTC SF

Data sets:
Data typeCount
13C chemical shifts91
15N chemical shifts6
1H chemical shifts212

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1Eurocin1

Entities:

Entity 1, Eurocin 42 residues - 4348.852 Da.

1   GLYPHEGLYCYSPROGLYASPALATYRGLN
2   CYSSERGLUHISCYSARGALALEUGLYGLY
3   GLYARGTHRGLYGLYTYRCYSALAGLYPRO
4   TRPTYRLEUGLYHISPROTHRCYSTHRCYS
5   SERPHE

Samples:

sample_1: Eurocin 1.68 mM; formic acid 95 mM; H2O 95%; D2O 5%

sample_conditions_1: pH: 4.5; pressure: 1 atm; temperature: 298.1 K

Experiments:

NameSampleSample stateSample conditions
2D 1H-15N HSQCsample_1isotropicsample_conditions_1
2D 1H-13C HSQC aliphaticsample_1isotropicsample_conditions_1
2D 1H-13C HSQC aromaticsample_1isotropicsample_conditions_1
2D 1H-1H TOCSYsample_1isotropicsample_conditions_1
2D DQF-COSYsample_1isotropicsample_conditions_1
2D 1H-1H NOESYsample_1isotropicsample_conditions_1

Software:

CYANA v2.1, Guntert, Mumenthaler and Wuthrich - structure solution

XEASY v1.5.5, Bartels et al. - chemical shift assignment

TOPSPIN v1.3, Bruker Biospin - collection

YASARA v12.1.19, Dr. Elmar Krieger - refinement

ProcheckNMR, Laskowski and MacArthur - Structure validation

NMR spectrometers:

  • Bruker DRX 600 MHz

Related Database Links:

PDB

Download HSQC peak lists in one of the following formats:
CSV: Backbone or all simulated peaks
SPARKY: Backbone or all simulated peaks