BMRB Entry 18459

Name: N0 domain of Neisseria meningitidis Pilus assembly protein PilQ
Published: 2013-01-03

Click here to enlarge.

PDB ID: 4ar0
Entry in NMR Restraints Grid
Validation report in NRG-CING
Chem Shift validation: AVS_full, LACS
BMRB Entry DOI: doi:10.13018/BMR18459
MolProbity Validation Chart

NMR-STAR file interactive viewer.
NMR-STAR v3 text file.
XML gzip file.
RDF gzip file.
All files associated with the entry

Nicholas Rego and David Koes
3Dmol.js: molecular visualization with WebGL
Bioinformatics (2015) 31 (8): 1322-1324 doi:10.1093/bioinformatics/btu829

Select model:

I am color blind

Download raw data. Direct link to this display. Help

Title:

N0 domain of Neisseria meningitidis Pilus assembly protein PilQ

Deposition date:

2012-05-11

Original release date:

2013-01-03

Authors:

Phelan, Marie; Berry, J.; Derrick, J.; Lian, L.

Citation:

Citation: Berry, Jamie-Lee; Phelan, Marie; Collins, Richard; Adomavicius, Tomas; Tnjum, Tone; Frye, Stefan; Bird, Louise; Owens, Ray; Ford, Robert; Lian, Lu-Yun; Derrick, Jeremy. "Structure and assembly of a trans-periplasmic channel for type IV pili in Neisseria meningitidis." PLoS Pathog. 8, .-. (2012).
PubMed: 23028322

Assembly members:

Assembly members:
TYPE_IV_PILUS_BIOGENESIS_AND_COMPETENCE_PROTEIN_P, polymer, 128 residues, 14465.3886 Da.

Natural source:

Natural source: Common Name: b-proteobacteria Taxonomy ID: 487 Superkingdom: Bacteria Kingdom: Neisseria Genus/species: Neisseria meningitidis

Experimental source:

Experimental source: Production method: recombinant technology Host organism: Escherichia coli Vector: pETM11

Entity Sequences (FASTA):

Entity Sequences (FASTA):
TYPE_IV_PILUS_BIOGENESIS_AND_COMPETENCE_PROTEIN_P: MKHHHHHHPMSDYDIPTTEN LYFEGAMGFTGRKISLDFQD VEIRTILQILAKESGMNIVA SDSVNGKMTLSLKDVPWDQA LDLVMQARNLDMRQQGNIVN IAPRDELLAKDKAFLQAEKD IADLGALY

Data sets:

assigned_chemical_shifts
- assigned_chem_shift_list

Data type	Count
13C chemical shifts	518
15N chemical shifts	133
1H chemical shifts	869

Additional metadata:

Assembly
Samples and Experiments
Software
Spectrometers
Hide all

Assembly:

Entity Assembly ID	Entity Name	Entity ID
1	TYPE IV PILUS BIOGENESIS AND COMPETENCE PROTEIN P	1

Entities:

Entity 1, TYPE IV PILUS BIOGENESIS AND COMPETENCE PROTEIN P 128 residues - 14465.3886 Da.

1

MET

LYS

HIS

PRO

MET

2

SER

ASP

TYR

ASP

ILE

PRO

THR

GLU

ASN

3

LEU

TYR

PHE

GLU

GLY

ALA

MET

GLY

PHE

THR

4

GLY

ARG

LYS

ILE

SER

LEU

ASP

PHE

GLN

ASP

5

VAL

GLU

ILE

ARG

THR

ILE

LEU

GLN

ILE

LEU

6

ALA

LYS

GLU

SER

GLY

MET

ASN

ILE

VAL

ALA

7

SER

ASP

SER

VAL

ASN

GLY

LYS

MET

THR

LEU

8

SER

LEU

LYS

ASP

VAL

PRO

TRP

ASP

GLN

ALA

9

LEU

ASP

LEU

VAL

MET

GLN

ALA

ARG

ASN

LEU

10

ASP

MET

ARG

GLN

GLY

ASN

ILE

VAL

ASN

11

ILE

ALA

PRO

ARG

ASP

GLU

LEU

ALA

LYS

12

ASP

LYS

ALA

PHE

LEU

GLN

ALA

GLU

LYS

ASP

13

ILE

ALA

ASP

LEU

GLY

ALA

LEU

TYR

Samples:

sample_1: TYPE IV PILUS BIOGENESIS AND COMPETENCE PROTEIN P, [U-13C; U-15N], 0.5 mM; H2O 10%; D2O 90%

sample_conditions_1: ionic strength: 100.000 mM; pH: 6.800; pressure: 1.000 atm; temperature: 298.000 K

Experiments:

Name	Sample	Sample state	Sample conditions
3D CBCA(CO)NH	sample_1	solution	sample_conditions_1
3D HNCACB	sample_1	solution	sample_conditions_1
3D HN(CA)CO 3D HNCO	sample_1	solution	sample_conditions_1
2D 1H-15N HSQC	sample_1	solution	sample_conditions_1
3D HBHA(CO)NH	sample_1	solution	sample_conditions_1
3D HBHANH	sample_1	solution	sample_conditions_1
2D hbCBcgcdHD	sample_1	solution	sample_conditions_1
2D ali 1H-13C HSQC	sample_1	solution	sample_conditions_1
2D aro 1H-13C HSQC	sample_1	solution	sample_conditions_1
3D ali HC-NOESY	sample_1	solution	sample_conditions_1
3D aro HC-NOESY	sample_1	solution	sample_conditions_1
3D HN-NOESY	sample_1	solution	sample_conditions_1
3D ali HCcH-TOCSY	sample_1	solution	sample_conditions_1
3D aro HCcH-TOCSY	sample_1	solution	sample_conditions_1
3D HNCACB	sample_1	solution	sample_conditions_1

Software:

AutoDep v4.3, PDBe - collection

CCPN_analysis vany, CCPN - chemical shift assignment

CNS1.2 vany, Brunger, Adams, Clore, Gros, Nilges and Read - chemical shift assignment

CYANA2.1 vany, Guntert, Mumenthaler and Wuthrich - chemical shift assignment

TALOSPLUS vany, Cornilescu, Delaglio and Bax - chemical shift assignment

Topspin2.1 vany, Bruker Biospin - chemical shift assignment

NMR spectrometers:

Bruker Avance 800 MHz
Bruker Avance 600 MHz
Bruker AVANCE 600 MHz

UNP	PILQ_NEIMB
BMRB	18428
PDB	4AR0
EMBL	CAM09716 CKK77493
AlphaFold	Q4W562