BMRB Entry 18449

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PDB ID:
Entry in NMR Restraints Grid
Validation report in NRG-CING
Chem Shift validation: AVS_full, SPARTA
BMRB Entry DOI: doi:10.13018/BMR18449
MolProbity Validation Chart

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Title:
Structure, sulfatide-binding properties, and inhibition of platelet aggregation by a Disabled-2-derived peptide
Deposition date:
2012-05-08
Original release date:
2012-09-17
Authors:
Xiao, Shuyan
Citation:

Citation: Xiao, Shuyan; Charonko, John; Fu, Xiangping; Salmanzadeh, Alireza; Davalos, Rafael; Vlachos, Pavlos; Finkielstein, Carla; Capelluto, Daniel. "Structure, sulfatide binding properties, and inhibition of platelet aggregation by a disabled-2 protein-derived peptide."  J. Biol. Chem. 287, 37691-37702 (2012).
PubMed: 22977233

Assembly members:

Assembly members:
SBM, polymer, 35 residues, 3993.754 Da.

Natural source:

Natural source:   Common Name: Humans   Taxonomy ID: 9606   Superkingdom: Eukaryota   Kingdom: Metazoa   Genus/species: Homo Sapiens

Experimental source:

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli   Vector: pgex-6p-1

Entity Sequences (FASTA):

Entity Sequences (FASTA):
SBM: SKKEKKKGPEKTDEYLLARF KGDGVKYKAKLIGID

Data sets:
Data typeCount
13C chemical shifts101
15N chemical shifts34
1H chemical shifts233

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
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Assembly:

Entity Assembly IDEntity NameEntity ID
1SBM1

Entities:

Entity 1, SBM 35 residues - 3993.754 Da.

1   SERLYSLYSGLULYSLYSLYSGLYPROGLU
2   LYSTHRASPGLUTYRLEULEUALAARGPHE
3   LYSGLYASPGLYVALLYSTYRLYSALALYS
4   LEUILEGLYILEASP

Samples:

sample_2: DSS, [U-2H], 50 uM; potassium chloride 40 mM; sodium azide 1 mM; citric, [U-99% 2H], 10 mM; SBM 1 mM

sample_1: DSS, [U-2H], 50 uM; potassium chloride 40 mM; sodium azide 1 mM; citric, [U-99% 2H], 10 mM; SBM 1 mM

sample_3: DSS, [U-2H], 50 uM; potassium chloride 40 mM; sodium azide 1 mM; citric, [U-99% 2H], 10 mM; SBM 1 mM

sample_conditions_1: pH: 5; temperature: 298 K

Experiments:

NameSampleSample stateSample conditions
3D HN(CA)COsample_3isotropicsample_conditions_1
3D HNCOsample_3isotropicsample_conditions_1
3D HNCACBsample_3isotropicsample_conditions_1
3D CBCA(CO)NHsample_3isotropicsample_conditions_1
3D 1H-15N NOESYsample_2isotropicsample_conditions_1
2D 1H-1H TOCSYsample_1isotropicsample_conditions_1
2D 1H-1H NOESYsample_1isotropicsample_conditions_1

Software:

X-PLOR NIH, Schwieters, Kuszewski, Tjandra and Clore - refinement

NMR spectrometers:

  • Bruker Avance 600 MHz

Related Database Links:

PDB
DBJ BAB22405 BAE32784 BAE37047 BAE37084 BAE38234
EMBL CAH89644
GB AAA98975 AAB02645 AAB02646 AAB02647 AAB19032
PIR A57542
REF NP_001008702 NP_001032994 NP_001095870 NP_001124738 NP_001180175
SP O88797 P98078 P98082
TPG DAA17841
AlphaFold O88797 P98078 P98082

Download HSQC peak lists in one of the following formats:
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SPARKY: Backbone or all simulated peaks