BMRB Entry 18448

Name: Chemical Shift Assignment of the NIPP1 FHA Domain
Published: 2013-02-14

Chem Shift validation: AVS_full, LACS
BMRB Entry DOI: doi:10.13018/BMR18448

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Nicholas Rego and David Koes
3Dmol.js: molecular visualization with WebGL
Bioinformatics (2015) 31 (8): 1322-1324 doi:10.1093/bioinformatics/btu829

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Title:

Chemical Shift Assignment of the NIPP1 FHA Domain

Deposition date:

2012-05-07

Original release date:

2013-02-14

Authors:

O'Connell, Nichole; Peti, Wolfgang

Citation:

Citation: Minnebo, Nikki; Gornemann, Janina; O'Connell, Nichole; Van Dessel, Nele; Derua, Rita; Vermunt, Marit; Page, Rebecca; Beullens, Monique; Peti, Wolfgang; Van Eynde, Aleyde; Bollen, Mathieu. "NIPP1 maintains EZH2 phosphorylation and promoter occupancy at proliferation-related target genes" Nucleic Acids Res. 41, 842-854 (2013).
PubMed: 23241245

Assembly members:

Assembly members:
NIPP1_FHA, polymer, 145 residues, Formula weight is not available

Natural source:

Natural source: Common Name: Human Taxonomy ID: 9606 Superkingdom: Eukaryota Kingdom: Metazoa Genus/species: Homo sapiens

Experimental source:

Experimental source: Production method: recombinant technology Host organism: Escherichia coli Vector: pET M30-MBP

Entity Sequences (FASTA):

Entity Sequences (FASTA):
NIPP1_FHA: GHMAAAANSGSSLPLFDCPT WAGKPPPGLHLDVVKGDKLI EKLIIDEKKYYLFGRNPDLC DFTIDHQSCSRVHAALVYHK HLKRVFLIDLNSTHGTFLGH IRLEPHKPQQIPIDSTVSFG ASTRAYTLREKPQTLPSAVK GDEKM

Data sets:

assigned_chemical_shifts
- assigned_chem_shift_list_1

Data type	Count
13C chemical shifts	258
15N chemical shifts	121
1H chemical shifts	121

Additional metadata:

Assembly
Samples and Experiments
Software
Spectrometers
Hide all

Assembly:

Entity Assembly ID	Entity Name	Entity ID
1	NIPP1 1-143	1

Entities:

Entity 1, NIPP1 1-143 145 residues - Formula weight is not available

GH are cloning artifacts. NIPP1 FHA domain residues 1-143

1

GLY

HIS

MET

ALA

ASN

SER

GLY

2

SER

LEU

PRO

LEU

PHE

ASP

CYS

PRO

THR

3

TRP

ALA

GLY

LYS

PRO

GLY

LEU

HIS

4

LEU

ASP

VAL

LYS

GLY

ASP

LYS

LEU

ILE

5

GLU

LYS

LEU

ILE

ASP

GLU

LYS

TYR

6

TYR

LEU

PHE

GLY

ARG

ASN

PRO

ASP

LEU

CYS

7

ASP

PHE

THR

ILE

ASP

HIS

GLN

SER

CYS

SER

8

ARG

VAL

HIS

ALA

LEU

VAL

TYR

HIS

LYS

9

HIS

LEU

LYS

ARG

VAL

PHE

LEU

ILE

ASP

LEU

10

ASN

SER

THR

HIS

GLY

THR

PHE

LEU

GLY

HIS

11

ILE

ARG

LEU

GLU

PRO

HIS

LYS

PRO

GLN

12

ILE

PRO

ILE

ASP

SER

THR

VAL

SER

PHE

GLY

13

ALA

SER

THR

ARG

ALA

TYR

THR

LEU

ARG

GLU

14

LYS

PRO

GLN

THR

LEU

PRO

SER

ALA

VAL

LYS

15

GLY

ASP

GLU

LYS

MET

Samples:

sample_1: NIPP1 FHA, [U-99% 13C; U-99% 15N], 1 mM; PMSF 0.25 mM; sodium chloride 50 mM; DTT 5 mM; BisTris 20 mM; H2O 90%; D2O 10%

sample_2: NIPP1 FHA, [U-99% 15N], 1 mM; PMSF 0.25 mM; sodium chloride 50 mM; DTT 5 mM; BisTris 20 mM; H2O 90%; D2O 10%

sample_conditions_1: ionic strength: 50 mM; pH: 6.5; pressure: 1 atm; temperature: 298 K

Experiments:

Name	Sample	Sample state	Sample conditions
2D 1H-15N HSQC	sample_2	isotropic	sample_conditions_1
3D HNCA	sample_1	isotropic	sample_conditions_1
3D HNCACB	sample_1	isotropic	sample_conditions_1
3D C(CO)NH	sample_1	isotropic	sample_conditions_1
3D CBCA(CO)NH	sample_1	isotropic	sample_conditions_1

Software:

TOPSPIN v2.1, Bruker Biospin - collection, processing

XEASY v1.8.4, Keller and Wuthrich - chemical shift assignment, peak picking

NMR spectrometers:

Bruker Avance 500 MHz

BMRB	15242
PDB	2JPE
DBJ	BAC27653 BAF84766 BAG65401 BAG73611
EMBL	CAA90625
GB	AAD22486 AAD24669 AAD31541 AAH01597 AAH25479
REF	NP_001026062 NP_001101381 NP_001230343 NP_001253391 NP_001277654
SP	Q12972 Q28147 Q8R3G1
TPG	DAA32062
AlphaFold	Q12972 Q28147 Q8R3G1