BMRB Entry 18448

Title:
Chemical Shift Assignment of the NIPP1 FHA Domain
Deposition date:
2012-05-07
Original release date:
2013-02-14
Authors:
O'Connell, Nichole; Peti, Wolfgang
Citation:

Citation: Minnebo, Nikki; Gornemann, Janina; O'Connell, Nichole; Van Dessel, Nele; Derua, Rita; Vermunt, Marit; Page, Rebecca; Beullens, Monique; Peti, Wolfgang; Van Eynde, Aleyde; Bollen, Mathieu. "NIPP1 maintains EZH2 phosphorylation and promoter occupancy at proliferation-related target genes"  Nucleic Acids Res. 41, 842-854 (2013).
PubMed: 23241245

Assembly members:

Assembly members:
NIPP1_FHA, polymer, 145 residues, Formula weight is not available

Natural source:

Natural source:   Common Name: Human   Taxonomy ID: 9606   Superkingdom: Eukaryota   Kingdom: Metazoa   Genus/species: Homo sapiens

Experimental source:

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli   Vector: pET M30-MBP

Data sets:
Data typeCount
13C chemical shifts258
15N chemical shifts121
1H chemical shifts121

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1NIPP1 1-1431

Entities:

Entity 1, NIPP1 1-143 145 residues - Formula weight is not available

GH are cloning artifacts. NIPP1 FHA domain residues 1-143

1   GLYHISMETALAALAALAALAASNSERGLY
2   SERSERLEUPROLEUPHEASPCYSPROTHR
3   TRPALAGLYLYSPROPROPROGLYLEUHIS
4   LEUASPVALVALLYSGLYASPLYSLEUILE
5   GLULYSLEUILEILEASPGLULYSLYSTYR
6   TYRLEUPHEGLYARGASNPROASPLEUCYS
7   ASPPHETHRILEASPHISGLNSERCYSSER
8   ARGVALHISALAALALEUVALTYRHISLYS
9   HISLEULYSARGVALPHELEUILEASPLEU
10   ASNSERTHRHISGLYTHRPHELEUGLYHIS
11   ILEARGLEUGLUPROHISLYSPROGLNGLN
12   ILEPROILEASPSERTHRVALSERPHEGLY
13   ALASERTHRARGALATYRTHRLEUARGGLU
14   LYSPROGLNTHRLEUPROSERALAVALLYS
15   GLYASPGLULYSMET

Samples:

sample_1: NIPP1 FHA, [U-99% 13C; U-99% 15N], 1 mM; PMSF 0.25 mM; sodium chloride 50 mM; DTT 5 mM; BisTris 20 mM; H2O 90%; D2O 10%

sample_2: NIPP1 FHA, [U-99% 15N], 1 mM; PMSF 0.25 mM; sodium chloride 50 mM; DTT 5 mM; BisTris 20 mM; H2O 90%; D2O 10%

sample_conditions_1: ionic strength: 50 mM; pH: 6.5; pressure: 1 atm; temperature: 298 K

Experiments:

NameSampleSample stateSample conditions
2D 1H-15N HSQCsample_2isotropicsample_conditions_1
3D HNCAsample_1isotropicsample_conditions_1
3D HNCACBsample_1isotropicsample_conditions_1
3D C(CO)NHsample_1isotropicsample_conditions_1
3D CBCA(CO)NHsample_1isotropicsample_conditions_1

Software:

TOPSPIN v2.1, Bruker Biospin - collection, processing

XEASY v1.8.4, Keller and Wuthrich - chemical shift assignment, peak picking

NMR spectrometers:

  • Bruker Avance 500 MHz

Related Database Links:

BMRB 15242
PDB
DBJ BAC27653 BAF84766 BAG65401 BAG73611
EMBL CAA90625
GB AAD22486 AAD24669 AAD31541 AAH01597 AAH25479
REF NP_001026062 NP_001101381 NP_001230343 NP_001253391 NP_001277654
SP Q12972 Q28147 Q8R3G1
TPG DAA32062
AlphaFold Q12972 Q28147 Q8R3G1

Download HSQC peak lists in one of the following formats:
CSV: Backbone or all simulated peaks
SPARKY: Backbone or all simulated peaks