BMRB Entry 18440

Title:
Analog of the fragment 197-221 of 1- adrenoreceptor
Deposition date:
2012-05-04
Original release date:
2013-05-03
Authors:
Bushuev, Valery; Zykov, K.; Bespalova, Z.; Bibilashvili, R.; Efremov, E.; Golitsyn, S.; Kaznacheeva, L.; Kuzhetsova, T.; Levashov, P.; Masenko, V.; Mironova, N.; Molokoedov, F.; Rutkevich, P.; Rvacheva, A.; Sharf, T.; Sidorova, M.; Vlasik, T.; Yanushevskaya, E.
Citation:

Citation: Bushuev, Valery. "Not applicable"  To be Published ., .-..

Assembly members:

Assembly members:
entity, polymer, 25 residues, 2941.249 Da.

Natural source:

Natural source:   Common Name: Human   Taxonomy ID: 9606   Superkingdom: Eukaryota   Kingdom: Metazoa   Genus/species: Homo sapiens

Experimental source:   Production method: chemical synthesis   Host organism: none   Vector: none

Experimental source:

Natural source:   Common Name: Human   Taxonomy ID: 9606   Superkingdom: Eukaryota   Kingdom: Metazoa   Genus/species: Homo sapiens

Experimental source:   Production method: chemical synthesis   Host organism: none   Vector: none

Entity Sequences (FASTA):

Entity Sequences (FASTA):
entity: NCWRAESDEARRCYNDPKCS DSVCK

Data sets:
Data typeCount
13C chemical shifts63
1H chemical shifts147

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1Analog of the fragment 197-221 of 1- adrenoreceptor1

Entities:

Entity 1, Analog of the fragment 197-221 of 1- adrenoreceptor 25 residues - 2941.249 Da.

1   ASNCYSTRPARGALAGLUSERASPGLUALA
2   ARGARGCYSTYRASNASPPROLYSCYSSER
3   ASPSERVALCYSLYS

Samples:

sample_1: entity 2 mM; sodium azide 1 mM; sodium acetate, [U-100% 2H], 20 mM; H2O 90%; D2O 10%

sample_2: entity 2 mM; sodium azide 1 mM; sodium acetate, [U-100% 2H], 20 mM; D2O 100%

sample_conditions_1: ionic strength: 21 mM; pH: 5.5; pressure: 1 atm; temperature: 303 K

Experiments:

NameSampleSample stateSample conditions
2D 1H-13C HSQC aliphaticsample_2isotropicsample_conditions_1
2D 1H-13C HSQC aromaticsample_2isotropicsample_conditions_1
2D DQF-COSYsample_2isotropicsample_conditions_1
2D 1H-1H NOESYsample_2isotropicsample_conditions_1
2D 1H-1H TOCSYsample_2isotropicsample_conditions_1
2D 1H-1H NOESYsample_1isotropicsample_conditions_1
2D 1H-1H TOCSYsample_1isotropicsample_conditions_1

Software:

xwinnmr v1.3, Bruker Biospin - collection, processing

CARA v1.9.5, Keller and Wuthrich - chemical shift assignment, peak picking

CYANA v3.0, Guntert, Mumenthaler and Wuthrich - structure solution

NMR spectrometers:

  • Bruker DPX 500 MHz

Related Database Links:

PDB