BMRB Entry 18424

Chem Shift validation: AVS_anomalous, AVS_full
BMRB Entry DOI: doi:10.13018/BMR18424

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Title:
Backbone dynamics of the truncated (2/2) hemoglobin from Synechococcus sp. PCC 7002 (ferric form without heme PTM, cyanide complex)
Deposition date:
2012-04-24
Original release date:
2012-05-02
Authors:
Lecomte, Juliette; Pond, Matthew; Majumdar, Ananya
Citation:

Citation: Wenke, Belinda; Lecomte, Juliette; Heroux, Annie; Schlessman, Jamie. "The 2/2 hemoglobin from the cyanobacterium Synechococcus sp. PCC 7002 with covalently attached heme: comparison of X-ray and NMR structures."  Proteins 82, 528-534 (2014).
PubMed: 23999883

Assembly members:

Assembly members:
entity_HEM, non-polymer, 616.487 Da.
GlbN, polymer, 123 residues, Formula weight is not available
entity_CYN, non-polymer, 26.017 Da.

Natural source:

Natural source:   Common Name: Synechococcus sp. PCC 7002   Taxonomy ID: 32049   Superkingdom: Bacteria   Kingdom: not available   Genus/species: cyanobacteria synechococcus

Experimental source:

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli   Vector: pET3c

Entity Sequences (FASTA):

Entity Sequences (FASTA):
GlbN: ASLYEKLGGAAAVDLAVEKF YGKVLADERVNRFFVNTDMA KQKQHQKDFMTYAFGGTDRF PGRSMRAAHQDLVENAGLTD VHFDAIAENLVLTLQELNVS QDLIDEVVTIVGSVQHRNDV LNR

Data sets:
Data typeCount
15N chemical shifts135
1H chemical shifts363
heteronuclear NOE values111
T1 relaxation values111
T2 relaxation values110

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1GlbN2
2prosthetic group1
3ligand3

Entities:

Entity 2, GlbN 123 residues - Formula weight is not available

1   ALASERLEUTYRGLULYSLEUGLYGLYALA
2   ALAALAVALASPLEUALAVALGLULYSPHE
3   TYRGLYLYSVALLEUALAASPGLUARGVAL
4   ASNARGPHEPHEVALASNTHRASPMETALA
5   LYSGLNLYSGLNHISGLNLYSASPPHEMET
6   THRTYRALAPHEGLYGLYTHRASPARGPHE
7   PROGLYARGSERMETARGALAALAHISGLN
8   ASPLEUVALGLUASNALAGLYLEUTHRASP
9   VALHISPHEASPALAILEALAGLUASNLEU
10   VALLEUTHRLEUGLNGLULEUASNVALSER
11   GLNASPLEUILEASPGLUVALVALTHRILE
12   VALGLYSERVALGLNHISARGASNASPVAL
13   LEUASNARG

Entity 1, prosthetic group - C34 H32 Fe N4 O4 - 616.487 Da.

1   HEM

Entity 3, ligand - C N - 26.017 Da.

1   CYN

Samples:

sample_1: GlbN, [U-15N], 0.9 mM; HEM 0.9 mM; potassium phosphate 20 mM; potassium cyanide 45 mM

sample_conditions_1: ionic strength: 0.2 M; pH: 7.3; pressure: 1 atm; temperature: 298 K

Experiments:

NameSampleSample stateSample conditions
2D 1H-15N HSQCsample_1isotropicsample_conditions_1
3D 1H-15N NOESYsample_1isotropicsample_conditions_1
3D 1H-15N TOCSYsample_1isotropicsample_conditions_1
2D DQF-COSYsample_1isotropicsample_conditions_1
2D 1H-1H NOESYsample_1isotropicsample_conditions_1
2D 1H-1H TOCSYsample_1isotropicsample_conditions_1
2D 1H-15N NOEsample_1isotropicsample_conditions_1
2D 15N R1sample_1isotropicsample_conditions_1
2D 15N R2sample_1isotropicsample_conditions_1

Software:

NMRPipe, Delaglio, Grzesiek, Vuister, Zhu, Pfeifer and Bax - processing

SPARKY, Goddard - chemical shift assignment

NMR spectrometers:

  • Bruker Avance 600 MHz
  • Varian INOVA 800 MHz

Related Database Links:

REF YP_001734867 WP_012307234 WP_030006991
BMRB 16306 16307 17947 18422 18423
PDB
GB AAL79195 ACA99611

Download HSQC peak lists in one of the following formats:
CSV: Backbone or all simulated peaks
SPARKY: Backbone or all simulated peaks