BMRB Entry 18403

Name: 1H,13C and 15N resonance assignment of the UIM-SH3 construct of the STAM2 protein
Published: 2012-07-17

Chem Shift validation: AVS_full
BMRB Entry DOI: doi:10.13018/BMR18403

NMR-STAR file interactive viewer.
NMR-STAR v3 text file.
XML gzip file.
RDF gzip file.
All files associated with the entry

Nicholas Rego and David Koes
3Dmol.js: molecular visualization with WebGL
Bioinformatics (2015) 31 (8): 1322-1324 doi:10.1093/bioinformatics/btu829

Select model:

I am color blind

Download raw data. Direct link to this display. Help

Title:

1H,13C and 15N resonance assignment of the UIM-SH3 construct of the STAM2 protein

Deposition date:

2012-04-16

Original release date:

2012-07-17

Authors:

Lange, Anja; Ismail, Mouhamad-Baligh; Riviere, Gwladys; Hologne, Maggy; Guilliere, Florence; Lancelin, Jean-Marc; Krimm, Isabelle; Walker, Olivier

Citation:

Citation: Lange, Anja; Ismail, Mouhamad-Baligh; Riviere, Gwladys; Hologne, Maggy; Lacabanne, Denis; Guilliere, Florence; Lancelin, Jean-Marc; Krimm, Isabelle; Walker, Olivier. "Competitive binding of UBPY and ubiquitin to the STAM2 SH3 domain revealed by NMR." FEBS Lett. 586, 3379-3384 (2012).
PubMed: 22841719

Assembly members:

Assembly members:
UIM-SH3_domains_of_STAM2, polymer, 109 residues, Formula weight is not available

Natural source:

Natural source: Common Name: Human Taxonomy ID: 9606 Superkingdom: Eukaryota Kingdom: Metazoa Genus/species: Homo sapiens

Experimental source:

Experimental source: Production method: recombinant technology Host organism: Escherichia coli Vector: pETEM-60

Entity Sequences (FASTA):

Entity Sequences (FASTA):
UIM-SH3_domains_of_STAM2: GAMGMNKNKEDEDIAKAIEL SLQEQKQQHTETKSLYPSSE IQLNNKVARKVRALYDFEAV EDNELTFKHGEIIIVLDDSD ANWWKGENHRGIGLFPSDFV TTNLNIETE

Data sets:

assigned_chemical_shifts
- assigned_chem_shift_list_1

Data type	Count
13C chemical shifts	194
15N chemical shifts	104
1H chemical shifts	104

Additional metadata:

Assembly
Samples and Experiments
Software
Spectrometers
Hide all

Assembly:

Entity Assembly ID	Entity Name	Entity ID
1	UIM-SH3 monomer	1

Entities:

Entity 1, UIM-SH3 monomer 109 residues - Formula weight is not available

1

GLY

ALA

MET

GLY

MET

ASN

LYS

ASN

LYS

GLU

2

ASP

GLU

ASP

ILE

ALA

LYS

ALA

ILE

GLU

LEU

3

SER

LEU

GLN

GLU

GLN

LYS

GLN

HIS

THR

4

GLU

THR

LYS

SER

LEU

TYR

PRO

SER

GLU

5

ILE

GLN

LEU

ASN

LYS

VAL

ALA

ARG

LYS

6

VAL

ARG

ALA

LEU

TYR

ASP

PHE

GLU

ALA

VAL

7

GLU

ASP

ASN

GLU

LEU

THR

PHE

LYS

HIS

GLY

8

GLU

ILE

VAL

LEU

ASP

SER

ASP

9

ALA

ASN

TRP

LYS

GLY

GLU

ASN

HIS

ARG

10

GLY

ILE

GLY

LEU

PHE

PRO

SER

ASP

PHE

VAL

11

THR

ASN

LEU

ASN

ILE

GLU

THR

GLU

Samples:

sample_1: UIM-SH3 domains of STAM2, [U-13C; U-15N], 200-400 uM; sodium phosphate 20 mM; sodium azide 0.02%

sample_conditions_1: ionic strength: 20 mM; pH: 6.8; pressure: 1 atm; temperature: 288 K

Experiments:

Name	Sample	Sample state	Sample conditions
3D HNCO	sample_1	isotropic	sample_conditions_1
3D HN(CO)CA	sample_1	isotropic	sample_conditions_1
2D 1H-15N HSQC	sample_1	isotropic	sample_conditions_1
3D 1H-13C NOESY	sample_1	isotropic	sample_conditions_1

Software:

VNMRJ, Varian - collection

NMRPipe, Delaglio, Grzesiek, Vuister, Zhu, Pfeifer and Bax - processing

NMRView, Johnson, One Moon Scientific - chemical shift assignment

NMR spectrometers:

Varian INOVA 600 MHz

PDB	1X2Q
DBJ	BAF84954 BAF85536 BAG72771 BAK62690
EMBL	CAB63735 CAL38297
GB	AAC63963 AAC63964 AAH28740 AAY14712 ABM83353
REF	NP_001267210 NP_005834 XP_003275024 XP_003776054 XP_003832773
SP	O75886
AlphaFold	O75886