BMRB Entry 18386

Name: Characterization of the chromoshadow domain-mediated binding of heterochromatin protein 1 alpha (HP1 alpha) to histone H3: Backbone 1H, 15N chemical shift assignments for HP1 CSDalpha(109-185)
Published: 2012-05-21

Chem Shift validation: AVS_full
BMRB Entry DOI: doi:10.13018/BMR18386

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Nicholas Rego and David Koes
3Dmol.js: molecular visualization with WebGL
Bioinformatics (2015) 31 (8): 1322-1324 doi:10.1093/bioinformatics/btu829

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Title:

Characterization of the chromoshadow domain-mediated binding of heterochromatin protein 1 alpha (HP1 alpha) to histone H3: Backbone 1H, 15N chemical shift assignments for HP1 CSDalpha(109-185)

Deposition date:

2012-04-10

Original release date:

2012-05-21

Authors:

Richart, Alexandria; Brunner, Clair; Stott, Katherine; Murzina, Natalia; Thomas, Jean

Citation:

Citation: Richart, Alexandria; Brunner, Clair; Stott, Katherine; Murzina, Natalia; Thomas, Jean. "Characterization of Chromoshadow Domain-mediated Binding of Heterochromatin Protein 1 (HP1) to Histone H3." J. Biol. Chem. 287, 18730-18737 (2012).
PubMed: 22493481

Assembly members:

Assembly members:
HP1_CSDalpha(109-185), polymer, 191 residues, Formula weight is not available

Natural source:

Natural source: Common Name: Humans Taxonomy ID: 9606 Superkingdom: Eukaryota Kingdom: Metazoa Genus/species: Homo sapiens

Experimental source:

Experimental source: Production method: recombinant technology Host organism: Escherichia coli Vector: pGEX2T

Entity Sequences (FASTA):

Entity Sequences (FASTA):
HP1_CSDalpha(109-185): MGKKTKRTADSSSSEDEEEY VVEKVLDRRVVKGQVEYLLK WKGFSEEHNTWEPEKNLDCP ELISEFMKKYKKMKEGENNK PREKSESNKRKSNFSNSADD IKSKKKREQSNDIARGFERG LEPEKIIGATDSCGDLMFLM KWKDTDEADLVLAKEANVKC PQIVIAFYEERLTWHAYPED AENKEKETAKS

Data sets:

assigned_chemical_shifts
- assigned_chem_shift_list_1

Data type	Count
15N chemical shifts	59
1H chemical shifts	61

Additional metadata:

Assembly
Samples and Experiments
Software
Spectrometers
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Assembly:

Entity Assembly ID	Entity Name	Entity ID
1	HP1 CSDalpha(109-185)	1

Entities:

Entity 1, HP1 CSDalpha(109-185) 191 residues - Formula weight is not available

1

MET

GLY

LYS

THR

LYS

ARG

THR

ALA

ASP

2

SER

GLU

ASP

GLU

TYR

3

VAL

GLU

LYS

VAL

LEU

ASP

ARG

VAL

4

VAL

LYS

GLY

GLN

VAL

GLU

TYR

LEU

LYS

5

TRP

LYS

GLY

PHE

SER

GLU

HIS

ASN

THR

6

TRP

GLU

PRO

GLU

LYS

ASN

LEU

ASP

CYS

PRO

7

GLU

LEU

ILE

SER

GLU

PHE

MET

LYS

TYR

8

LYS

MET

LYS

GLU

GLY

GLU

ASN

LYS

9

PRO

ARG

GLU

LYS

SER

GLU

SER

ASN

LYS

ARG

10

LYS

SER

ASN

PHE

SER

ASN

SER

ALA

ASP

11

ILE

LYS

SER

LYS

ARG

GLU

GLN

SER

12

ASN

ASP

ILE

ALA

ARG

GLY

PHE

GLU

ARG

GLY

13

LEU

GLU

PRO

GLU

LYS

ILE

GLY

ALA

THR

14

ASP

SER

CYS

GLY

ASP

LEU

MET

PHE

LEU

MET

15

LYS

TRP

LYS

ASP

THR

ASP

GLU

ALA

ASP

LEU

16

VAL

LEU

ALA

LYS

GLU

ALA

ASN

VAL

LYS

CYS

17

PRO

GLN

ILE

VAL

ILE

ALA

PHE

TYR

GLU

18

ARG

LEU

THR

TRP

HIS

ALA

TYR

PRO

GLU

ASP

19

ALA

GLU

ASN

LYS

GLU

LYS

GLU

THR

ALA

LYS

20

SER

Samples:

sample_1: HP1_CSDalpha(109-185), [U-95% 15N], 0.5 mM; sodium phosphate 10 mM; EDTA 1 mM; DTT 1 mM; H2O 90%; D2O 10%

sample_conditions_1: ionic strength: 0.01 M; pH: 8.0; pressure: 1 atm; temperature: 298 K

Experiments:

Name	Sample	Sample state	Sample conditions
2D 1H-15N HSQC	sample_1	isotropic	sample_conditions_1
3D 1H-15N TOCSY	sample_1	isotropic	sample_conditions_1
3D 1H-15N NOESY	sample_1	isotropic	sample_conditions_1

Software:

AZARA, Boucher - processing

Analysis, CCPN - chemical shift assignment

NMR spectrometers:

Bruker DRX 600 MHz

DBJ	BAB25897 BAB31173 BAC26923 BAC26966 BAC28107
EMBL	CAA67960 CAG33699
GB	AAA72327 AAB26994 AAC50553 AAF80993 AAH04707
REF	NP_001070257 NP_001100267 NP_001103686 NP_001120793 NP_001120794
SP	P45973 Q61686
TPG	DAA30037 DAA30038 DAA30039 DAA30040
AlphaFold	P45973 Q61686