BMRB

Biological Magnetic Resonance Data Bank


A Repository for Data from NMR Spectroscopy on Proteins, Peptides, Nucleic Acids, and other Biomolecules
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BMRB Entry 18373

Title: bLac - Oceanobacillus iheyensis

Deposition date: 2012-04-02 Original release date: 2012-05-01

Authors: Ortega, Gabriel; Millet, Oscar

Citation: Ortega, Gabriel. "Exchange"  J. Am. Chem. Soc. ., .-..

Assembly members:
beta_Lac_Oih, polymer, 261 residues, 29000 Da.

Natural source:   Common Name: Oceanobacillus iheyensis   Taxonomy ID: 182710   Superkingdom: Bacteria   Kingdom: not available   Genus/species: Oceanobacillus iheyensis

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli   Vector: pET-16b

Entity Sequences (FASTA):
beta_Lac_Oih: MGSEDLKKLEEEFDVRLGVY AIDTGADKEISYRENERFAY TSTFKPLAVGAVLQTKSDEE LEETITYSEEDLVTYSPITE QHVDEGMTLVEIADAAIRYS DNTAGNLLLEAMGGPDELET ILRDIGDETIEMDRYETELN EAKPGDIRDTSTAKAMATTL QQYVLEDVLDADRREVLTNM LINNTTGDALIRAGVPDGWT VGDKTGAGGYGTRNDIGIIW PEGDEEPIVIAIMSSRDEED ADYDDKLIEKATEIVLQELR N

Data sets:
Data typeCount
13C chemical shifts444
15N chemical shifts229
1H chemical shifts229

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1bLAC1

Entities:

Entity 1, bLAC 261 residues - 29000 Da.

1   METGLYSERGLUASPLEULYSLYSLEUGLU
2   GLUGLUPHEASPVALARGLEUGLYVALTYR
3   ALAILEASPTHRGLYALAASPLYSGLUILE
4   SERTYRARGGLUASNGLUARGPHEALATYR
5   THRSERTHRPHELYSPROLEUALAVALGLY
6   ALAVALLEUGLNTHRLYSSERASPGLUGLU
7   LEUGLUGLUTHRILETHRTYRSERGLUGLU
8   ASPLEUVALTHRTYRSERPROILETHRGLU
9   GLNHISVALASPGLUGLYMETTHRLEUVAL
10   GLUILEALAASPALAALAILEARGTYRSER
11   ASPASNTHRALAGLYASNLEULEULEUGLU
12   ALAMETGLYGLYPROASPGLULEUGLUTHR
13   ILELEUARGASPILEGLYASPGLUTHRILE
14   GLUMETASPARGTYRGLUTHRGLULEUASN
15   GLUALALYSPROGLYASPILEARGASPTHR
16   SERTHRALALYSALAMETALATHRTHRLEU
17   GLNGLNTYRVALLEUGLUASPVALLEUASP
18   ALAASPARGARGGLUVALLEUTHRASNMET
19   LEUILEASNASNTHRTHRGLYASPALALEU
20   ILEARGALAGLYVALPROASPGLYTRPTHR
21   VALGLYASPLYSTHRGLYALAGLYGLYTYR
22   GLYTHRARGASNASPILEGLYILEILETRP
23   PROGLUGLYASPGLUGLUPROILEVALILE
24   ALAILEMETSERSERARGASPGLUGLUASP
25   ALAASPTYRASPASPLYSLEUILEGLULYS
26   ALATHRGLUILEVALLEUGLNGLULEUARG
27   ASN

Samples:

sample_1: beta Lac Oih, [U-100% 13C; U-100% 15N; U-80% 2H], 0.9 mM; Phosphate 20 mM; NaCl 500 mM; H2O 90%; D2O 10%

sample_conditions_1: ionic strength: 0.52 M; pH: 7.5; pressure: 1 atm; temperature: 298 K

Experiments:

NameSampleSample stateSample conditions
2D 1H-15N HSQCsample_1isotropicsample_conditions_1
3D HNCOsample_1isotropicsample_conditions_1
3D HNCAsample_1isotropicsample_conditions_1
3D HNCACBsample_1isotropicsample_conditions_1
3D HN(COCA)CBsample_1isotropicsample_conditions_1
3D HN(CO)CAsample_1isotropicsample_conditions_1

Software:

TOPSPIN, Bruker Biospin - collection

NMR spectrometers:

  • Bruker Avance 800 MHz

Related Database Links:

PDB
DBJ BAC14749
GB ACQ42223
REF WP_041544309

Download simulated HSQC data in one of the following formats:
CSV: Backbone or all simulated shifts
SPARKY: Backbone or all simulated shifts