BMRB Entry 18373

Name: bLac - Oceanobacillus iheyensis
Published: 2012-05-01

Chem Shift validation: AVS_full
BMRB Entry DOI: doi:10.13018/BMR18373

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Nicholas Rego and David Koes
3Dmol.js: molecular visualization with WebGL
Bioinformatics (2015) 31 (8): 1322-1324 doi:10.1093/bioinformatics/btu829

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Title:

bLac - Oceanobacillus iheyensis

Deposition date:

2012-04-02

Original release date:

2012-05-01

Authors:

Ortega, Gabriel; Millet, Oscar

Citation:

Citation: Ortega, Gabriel. "Exchange" J. Am. Chem. Soc. ., .-..

Assembly members:

Assembly members:
beta_Lac_Oih, polymer, 261 residues, 29000 Da.

Natural source:

Natural source: Common Name: Oceanobacillus iheyensis Taxonomy ID: 182710 Superkingdom: Bacteria Kingdom: not available Genus/species: Oceanobacillus iheyensis

Experimental source:

Experimental source: Production method: recombinant technology Host organism: Escherichia coli Vector: pET-16b

Entity Sequences (FASTA):

Entity Sequences (FASTA):
beta_Lac_Oih: MGSEDLKKLEEEFDVRLGVY AIDTGADKEISYRENERFAY TSTFKPLAVGAVLQTKSDEE LEETITYSEEDLVTYSPITE QHVDEGMTLVEIADAAIRYS DNTAGNLLLEAMGGPDELET ILRDIGDETIEMDRYETELN EAKPGDIRDTSTAKAMATTL QQYVLEDVLDADRREVLTNM LINNTTGDALIRAGVPDGWT VGDKTGAGGYGTRNDIGIIW PEGDEEPIVIAIMSSRDEED ADYDDKLIEKATEIVLQELR N

Data sets:

assigned_chemical_shifts
- assigned_chem_shift_list_1

Data type	Count
13C chemical shifts	444
15N chemical shifts	229
1H chemical shifts	229

Additional metadata:

Assembly
Samples and Experiments
Software
Spectrometers
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Assembly:

Entity Assembly ID	Entity Name	Entity ID
1	bLAC	1

Entities:

Entity 1, bLAC 261 residues - 29000 Da.

1

MET

GLY

SER

GLU

ASP

LEU

LYS

LEU

GLU

2

GLU

PHE

ASP

VAL

ARG

LEU

GLY

VAL

TYR

3

ALA

ILE

ASP

THR

GLY

ALA

ASP

LYS

GLU

ILE

4

SER

TYR

ARG

GLU

ASN

GLU

ARG

PHE

ALA

TYR

5

THR

SER

THR

PHE

LYS

PRO

LEU

ALA

VAL

GLY

6

ALA

VAL

LEU

GLN

THR

LYS

SER

ASP

GLU

7

LEU

GLU

THR

ILE

THR

TYR

SER

GLU

8

ASP

LEU

VAL

THR

TYR

SER

PRO

ILE

THR

GLU

9

GLN

HIS

VAL

ASP

GLU

GLY

MET

THR

LEU

VAL

10

GLU

ILE

ALA

ASP

ALA

ILE

ARG

TYR

SER

11

ASP

ASN

THR

ALA

GLY

ASN

LEU

GLU

12

ALA

MET

GLY

PRO

ASP

GLU

LEU

GLU

THR

13

ILE

LEU

ARG

ASP

ILE

GLY

ASP

GLU

THR

ILE

14

GLU

MET

ASP

ARG

TYR

GLU

THR

GLU

LEU

ASN

15

GLU

ALA

LYS

PRO

GLY

ASP

ILE

ARG

ASP

THR

16

SER

THR

ALA

LYS

ALA

MET

ALA

THR

LEU

17

GLN

TYR

VAL

LEU

GLU

ASP

VAL

LEU

ASP

18

ALA

ASP

ARG

GLU

VAL

LEU

THR

ASN

MET

19

LEU

ILE

ASN

THR

GLY

ASP

ALA

LEU

20

ILE

ARG

ALA

GLY

VAL

PRO

ASP

GLY

TRP

THR

21

VAL

GLY

ASP

LYS

THR

GLY

ALA

GLY

TYR

22

GLY

THR

ARG

ASN

ASP

ILE

GLY

ILE

TRP

23

PRO

GLU

GLY

ASP

GLU

PRO

ILE

VAL

ILE

24

ALA

ILE

MET

SER

ARG

ASP

GLU

ASP

25

ALA

ASP

TYR

ASP

LYS

LEU

ILE

GLU

LYS

26

ALA

THR

GLU

ILE

VAL

LEU

GLN

GLU

LEU

ARG

27

ASN

Samples:

sample_1: beta Lac Oih, [U-100% 13C; U-100% 15N; U-80% 2H], 0.9 mM; Phosphate 20 mM; NaCl 500 mM; H2O 90%; D2O 10%

sample_conditions_1: ionic strength: 0.52 M; pH: 7.5; pressure: 1 atm; temperature: 298 K

Experiments:

Name	Sample	Sample state	Sample conditions
2D 1H-15N HSQC	sample_1	isotropic	sample_conditions_1
3D HNCO	sample_1	isotropic	sample_conditions_1
3D HNCA	sample_1	isotropic	sample_conditions_1
3D HNCACB	sample_1	isotropic	sample_conditions_1
3D HN(COCA)CB	sample_1	isotropic	sample_conditions_1
3D HN(CO)CA	sample_1	isotropic	sample_conditions_1

Software:

TOPSPIN, Bruker Biospin - collection

NMR spectrometers:

Bruker Avance 800 MHz

PDB	3LEZ
DBJ	BAC14749
GB	ACQ42223
REF	WP_041544309