BMRB Entry 18364

Title:
NMR structure of the protein YP_001300941.1 from Bacteroides vulgatus
Deposition date:
2012-03-29
Original release date:
2012-04-30
Authors:
Dutta, Samit; Serrano, Pedro; Geralt, Michael; Wuthrich, Kurt; JCSG, JCSG
Citation:

Citation: Dutta, Samit; Serrano, Pedro; Geralt, Michael; Wuthrich, Kurt. "NMR structure of the protein YP_001300941.1 from Bacteroides vulgatus"  .

Assembly members:

Assembly members:
GS13500A, polymer, 126 residues, 13488.005 Da.

Natural source:

Natural source:   Common Name: Bacteroides vulgates   Taxonomy ID: 821   Superkingdom: Bacteria   Kingdom: not available   Genus/species: Bacteroides vulgates

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli   Vector: SpeedET

Experimental source:

Natural source:   Common Name: Bacteroides vulgates   Taxonomy ID: 821   Superkingdom: Bacteria   Kingdom: not available   Genus/species: Bacteroides vulgates

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli   Vector: SpeedET

Data sets:
Data typeCount
13C chemical shifts395
15N chemical shifts130
1H chemical shifts790

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1GS13500A1

Entities:

Entity 1, GS13500A 126 residues - 13488.005 Da.

1   GLYALASERASPPHEGLNTHRGLYILEHIS
2   LYSILEVALILEGLNGLNSERGLYASPTHR
3   ASPSERPHEGLUVALSERVALSERILEGLY
4   GLYALAASPLYSGLYGLYPROALALYSLEU
5   TYRASNASPLYSGLYGLUTYRILEGLYASP
6   SERTYRSERALAGLNILEARGTHRALATHR
7   METSERCYSCYSTHRASNGLYASNALAPHE
8   PHEMETTHRCYSALAGLYSERVALSERSER
9   ILESERGLUALAGLYLYSARGLEUHISILE
10   THRVALILEGLYTYRILEASPASPLYSGLU
11   VALASNARGLEUGLULYSGLUTYRILETHR
12   ASPGLYASNTHRLEUILEGLUTHRPHESER
13   VALSERTHRLYSGLUILE

Samples:

sample_1: GS13500A, [U-98% 13C; U-98% 15N], 1.2 mM; sodium phosphate 20 mM; sodium chloride 50 mM; H2O 95 mM; D2O, [U-99% 2H], 5 mM

sample_conditions_1: ionic strength: 0.08 M; pH: 6.0; pressure: 1 atm; temperature: 298 K

Experiments:

NameSampleSample stateSample conditions
2D 1H-15N HSQCsample_1isotropicsample_conditions_1
4D APSY-HACANHsample_1isotropicsample_conditions_1
5D APSY-HACACONHsample_1isotropicsample_conditions_1
5D APSY-CBCACONHsample_1isotropicsample_conditions_1
3D 1H-15N NOESYsample_1isotropicsample_conditions_1
3D 1H-13C NOESY aliphaticsample_1isotropicsample_conditions_1
3D 1H-13C NOESY aromaticsample_1isotropicsample_conditions_1

Software:

TOPSPIN v2.1, Bruker Biospin - collection, processing

CARA, Keller and Wuthrich - chemical shift assignment, data analysis

CYANA, Guntert, Mumenthaler and Wuthrich - refinement, structure solution

OPAL, Luginbuhl, Guntert, Billeter and Wuthrich - refinement

UNIO, (Unio)-Herrmann and Wuthrich - chemical shift assignment, structure solution

NMR spectrometers:

  • Bruker Avance 600 MHz
  • Bruker Avance 800 MHz

Related Database Links:

PDB
EMBL CBW24855 CUO17365 CUQ42974
GB ABR41319 ALK85811 EES67826 EFI05346 EFI12350
REF WP_005805239 WP_005823213 WP_008640853 WP_008766551 WP_009040585

Download HSQC peak lists in one of the following formats:
CSV: Backbone or all simulated peaks
SPARKY: Backbone or all simulated peaks