BMRB Entry 18356

Name: Solution Structure of ADF like UNC-60A Protein of Caenorhabditis elegans
Published: 2013-05-20

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PDB ID: 2mp4
Entry in NMR Restraints Grid
Validation report in NRG-CING
Chem Shift validation: AVS_anomalous, AVS_full, LACS, SPARTA
BMRB Entry DOI: doi:10.13018/BMR18356
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Bioinformatics (2015) 31 (8): 1322-1324 doi:10.1093/bioinformatics/btu829

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Title:

Solution Structure of ADF like UNC-60A Protein of Caenorhabditis elegans

Deposition date:

2012-03-28

Original release date:

2013-05-20

Authors:

Shukla, Vaibhav Kumar; Yadav, Rahul; Kabra, Ashish; Kumar, Dinesh; Ono, Shoichiro; Arora, Ashish

Citation:

Citation: Shukla, Vaibhav Kumar; Yadav, Rahul; Jain, Anupam; Tripathi, Sarita; Kabra, Ashish; Kumar, Dinesh; Ono, Shoichiro; Arora, Ashish. "NMR Structure and Backbone dynamics of ADF like UNC-60A protein from Caenorhabditis elegans: its divergence from conventional ADF/cofilin" J. Biol. Chem. ., .-..

Assembly members:

Assembly members:
entity, polymer, 165 residues, 18391.953 Da.

Natural source:

Natural source: Common Name: Caenorhabditis elegans Taxonomy ID: 6239 Superkingdom: Eukaryota Kingdom: Metazoa Genus/species: Caenorhabditis elegans

Experimental source:

Experimental source: Production method: recombinant technology Host organism: Escherichia coli Vector: pETNH6

Entity Sequences (FASTA):

Entity Sequences (FASTA):
entity: MSSGVMVDPDVQTSFQKLSE GRKEYRYIIFKIDENKVIVE AAVTQDQLGITGDDYDDSSK AAFDKFVEDVKSRTDNLTDC RYAVFDFKFTCSRVGAGTSK MDKIIFLQICPDGASIKKKM VYASSAAAIKTSLGTGKILQ FQVSDESEMSHKELLNKLGE KYGDH

Data sets:

assigned_chemical_shifts
- assigned_chem_shift_list_1

Data type	Count
13C chemical shifts	719
15N chemical shifts	162
1H chemical shifts	1114

Additional metadata:

Assembly
Samples and Experiments
Software
Spectrometers
Hide all

Assembly:

Entity Assembly ID	Entity Name	Entity ID
1	ADF like UNC-60A Protein	1

Entities:

Entity 1, ADF like UNC-60A Protein 165 residues - 18391.953 Da.

1

MET

SER

GLY

VAL

MET

VAL

ASP

PRO

ASP

2

VAL

GLN

THR

SER

PHE

GLN

LYS

LEU

SER

GLU

3

GLY

ARG

LYS

GLU

TYR

ARG

TYR

ILE

PHE

4

LYS

ILE

ASP

GLU

ASN

LYS

VAL

ILE

VAL

GLU

5

ALA

VAL

THR

GLN

ASP

GLN

LEU

GLY

ILE

6

THR

GLY

ASP

TYR

ASP

SER

LYS

7

ALA

PHE

ASP

LYS

PHE

VAL

GLU

ASP

VAL

8

LYS

SER

ARG

THR

ASP

ASN

LEU

THR

ASP

CYS

9

ARG

TYR

ALA

VAL

PHE

ASP

PHE

LYS

PHE

THR

10

CYS

SER

ARG

VAL

GLY

ALA

GLY

THR

SER

LYS

11

MET

ASP

LYS

ILE

PHE

LEU

GLN

ILE

CYS

12

PRO

ASP

GLY

ALA

SER

ILE

LYS

MET

13

VAL

TYR

ALA

SER

ALA

ILE

LYS

14

THR

SER

LEU

GLY

THR

GLY

LYS

ILE

LEU

GLN

15

PHE

GLN

VAL

SER

ASP

GLU

SER

GLU

MET

SER

16

HIS

LYS

GLU

LEU

ASN

LYS

LEU

GLY

GLU

17

LYS

TYR

GLY

ASP

HIS

Samples:

sample_1: UNC-60A, [U-99% 15N], 0.65 mM; sodium phosphate 20 mM; sodium chloride 50 mM; sodium azide 0.1%; D2O, [U-99% 2H], 7%; H2O 93%

sample_2: UNC-60A, [U-99% 13C; U-99% 15N], 0.65 mM; sodium phosphate 20 mM; sodium chloride 50 mM; sodium azide 0.1%; D2O, [U-99% 2H], 7%; H2O 93%

sample_3: UNC-60A, [U-99% 13C; U-99% 15N], 0.65 mM; sodium phosphate 20 mM; sodium chloride 50 mM; sodium azide 0.1%; D2O 100%

sample_conditions_1: ionic strength: 50 mM; pH: 6.5; pressure: 1 atm; temperature: 298 K

Experiments:

Name	Sample	Sample state	Sample conditions
2D 1H-15N HSQC	sample_1	isotropic	sample_conditions_1
3D HNCACB	sample_2	isotropic	sample_conditions_1
3D CBCA(CO)NH	sample_2	isotropic	sample_conditions_1
3D HNCO	sample_2	isotropic	sample_conditions_1
3D HNCA	sample_2	isotropic	sample_conditions_1
3D C(CO)NH	sample_2	isotropic	sample_conditions_1
3D H(CCO)NH	sample_2	isotropic	sample_conditions_1
3D HCCH-TOCSY	sample_3	isotropic	sample_conditions_1
2D 1H-13C HSQC aliphatic	sample_3	isotropic	sample_conditions_1
2D 1H-13C HSQC aromatic	sample_3	isotropic	sample_conditions_1
3D 1H-15N NOESY	sample_1	isotropic	sample_conditions_1
3D 1H-13C NOESY aliphatic	sample_3	isotropic	sample_conditions_1
3D 1H-13C NOESY aromatic	sample_3	isotropic	sample_conditions_1
2D CB(CGCDCE)HE	sample_2	isotropic	sample_conditions_1
2D CB(CGCD)HD	sample_2	isotropic	sample_conditions_1
3D HNCACO	sample_2	isotropic	sample_conditions_1

Software:

NMRPipe, Delaglio, Grzesiek, Vuister, Zhu, Pfeifer and Bax - processing

CARA, Keller and Wuthrich - data analysis

PSVS v1.4, Bhattacharya and Montelione - Validation of structural parameter

iCING, Doreleijers - Validation of structural parameter

CYANA v3.0, Guntert, Mumenthaler and Wuthrich - Structure Calculation

NMR spectrometers:

Varian INOVA 600 MHz
Bruker Avance3 800 MHz

PDB	2MP4
EMBL	CCD67021
GB	AAC14458
REF	NP_503425
SP	Q07750
AlphaFold	Q07750