BMRB Entry 18344

Chem Shift validation: AVS_full, LACS
BMRB Entry DOI: doi:10.13018/BMR18344

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Title:
Gal-GalNac-Interferon Alpha-2a
Deposition date:
2012-03-21
Original release date:
2013-01-03
Authors:
Ghasriani, Houman; Belcourt, Pascal; Sauve, Simon; Hodgson, Derek; Gingras, Genevieve; Brochu, Denis; Gilbert, Michel; Aubin, Yves
Citation:

Citation: Ghasriani, Houman; Belcourt, Pascal; Sauve, Simon; Hodgson, Derek; Brochu, Denis; Gilbert, Michel; Aubin, Yves. "A single N-acetylgalactosamine residue at threonine 106 modifies the dynamics and structure of interferon 2a around the glycosylation site."  J. Biol. Chem. 288, 247-254 (2013).
PubMed: 23184955

Assembly members:

Assembly members:
ggIFN_alpha-2a, polymer, 165 residues, Formula weight is not available
D-Gal-beta(1-3)GalNAc-alpha-Thr, non-polymer, Formula weight is not available

Natural source:

Natural source:   Common Name: Human   Taxonomy ID: 9606   Superkingdom: Eukaryota   Kingdom: Metazoa   Genus/species: Homo sapiens

Experimental source:

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli   Vector: pET-15b

Entity Sequences (FASTA):

Entity Sequences (FASTA):
ggIFN_alpha-2a: CDLPQTHSLGSRRTLMLLAQ MRKISLFSCLKDRHDFGFPQ EEFGNQFQKAETIPVLHEMI QQIFNLFSTKDSSAAWDETL LDKFYTELYQQLNDLEACVI QGVGVTETPLMKEDSILAVR KYFQRITLYLKEKKYSPCAW EVVRAEIMRSFSLSTNLQES LRSKE

Data sets:
Data typeCount
13C chemical shifts487
15N chemical shifts177
1H chemical shifts192

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1Entity_11
2GalGalNac2

Entities:

Entity 1, Entity_1 165 residues - Formula weight is not available

1   CYSASPLEUPROGLNTHRHISSERLEUGLY
2   SERARGARGTHRLEUMETLEULEUALAGLN
3   METARGLYSILESERLEUPHESERCYSLEU
4   LYSASPARGHISASPPHEGLYPHEPROGLN
5   GLUGLUPHEGLYASNGLNPHEGLNLYSALA
6   GLUTHRILEPROVALLEUHISGLUMETILE
7   GLNGLNILEPHEASNLEUPHESERTHRLYS
8   ASPSERSERALAALATRPASPGLUTHRLEU
9   LEUASPLYSPHETYRTHRGLULEUTYRGLN
10   GLNLEUASNASPLEUGLUALACYSVALILE
11   GLNGLYVALGLYVALTHRGLUTHRPROLEU
12   METLYSGLUASPSERILELEUALAVALARG
13   LYSTYRPHEGLNARGILETHRLEUTYRLEU
14   LYSGLULYSLYSTYRSERPROCYSALATRP
15   GLUVALVALARGALAGLUILEMETARGSER
16   PHESERLEUSERTHRASNLEUGLNGLUSER
17   LEUARGSERLYSGLU

Entity 2, GalGalNac - Formula weight is not available

Samples:

sample_1: ggIFN alpha-2a, [U-100% 13C; U-100% 15N], 0.85 mM; H2O 90%; D2O 10%

sample_conditions_1: ionic strength: 25 mM; pH: 3.5; pressure: 1 atm; temperature: 298 K

Experiments:

NameSampleSample stateSample conditions
2D 1H-15N HSQCsample_1isotropicsample_conditions_1
3D HNCOsample_1isotropicsample_conditions_1
3D HN(CA)COsample_1isotropicsample_conditions_1
3D HNCAsample_1isotropicsample_conditions_1
3D CBCA(CO)NHsample_1isotropicsample_conditions_1
3D HNCACBsample_1isotropicsample_conditions_1

Software:

SPARKY v3.115, Goddard - chemical shift assignment

NMR spectrometers:

  • Bruker Avance 600 MHz

Related Database Links:

BMRB 16677 18135 4081
PDB
DBJ BAJ20902
EMBL CAA23805 CAA23809 CAA23810 CAA25770 CAA46954
GB AAA52715 AAA59181 AAB59402 AAC64915 AAH74936
PRF 0611561A 0906342A
REF NP_000596 XP_003830724 XP_004047919 XP_528568
SP P01563
AlphaFold P01563

Download HSQC peak lists in one of the following formats:
CSV: Backbone or all simulated peaks
SPARKY: Backbone or all simulated peaks