BMRB Entry 18297

Name: E2 binding surface on Uba3 beta-grasp domain undergoes a conformational transition
Published: 2012-08-29

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PDB ID: 2lq7
Entry in NMR Restraints Grid
Validation report in NRG-CING
Chem Shift validation: AVS_full, LACS, SPARTA
BMRB Entry DOI: doi:10.13018/BMR18297
MolProbity Validation Chart

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Nicholas Rego and David Koes
3Dmol.js: molecular visualization with WebGL
Bioinformatics (2015) 31 (8): 1322-1324 doi:10.1093/bioinformatics/btu829

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Title:

E2 binding surface on Uba3 beta-grasp domain undergoes a conformational transition

Deposition date:

2012-02-27

Original release date:

2012-08-29

Authors:

Elgin, Emine; Peterson, Francis; Volkman, Brian

Citation:

Citation: Elgin, E. Sonay; Sokmen, Nazl; Peterson, Francis; Volkman, Brian; Da, Cada; Haas, Arthur. "E2-binding surface on Uba3 -grasp domain undergoes a conformational transition." Proteins 80, 2482-2487 (2012).
PubMed: 22821745

Assembly members:

Assembly members:
Uba3, polymer, 97 residues, 10658.173 Da.

Natural source:

Natural source: Common Name: Humans Taxonomy ID: 9606 Superkingdom: Eukaryota Kingdom: Metazoa Genus/species: Homo sapiens

Experimental source:

Experimental source: Production method: recombinant technology Host organism: Escherichia coli Vector: pQE30

Entity Sequences (FASTA):

Entity Sequences (FASTA):
Uba3: GSQLPQNIQFSPSAKLQEVL DYLTNSASLQMKSPAITATL EGKNRTLYLQSVTSIEERTR PNLSKTLKELGLVDGQELAV ADVTTPQTVLFKLHFTS

Data sets:

assigned_chemical_shifts
- assigned_chem_shift_list_1

Data type	Count
13C chemical shifts	367
15N chemical shifts	82
1H chemical shifts	591

Additional metadata:

Assembly
Samples and Experiments
Software
Spectrometers
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Assembly:

Entity Assembly ID	Entity Name	Entity ID
1	Uba3	1

Entities:

Entity 1, Uba3 97 residues - 10658.173 Da.

The N-terminal GS dipeptide is a cloning artifact.

1

GLY

SER

GLN

LEU

PRO

GLN

ASN

ILE

GLN

PHE

2

SER

PRO

SER

ALA

LYS

LEU

GLN

GLU

VAL

LEU

3

ASP

TYR

LEU

THR

ASN

SER

ALA

SER

LEU

GLN

4

MET

LYS

SER

PRO

ALA

ILE

THR

ALA

THR

LEU

5

GLU

GLY

LYS

ASN

ARG

THR

LEU

TYR

LEU

GLN

6

SER

VAL

THR

SER

ILE

GLU

ARG

THR

ARG

7

PRO

ASN

LEU

SER

LYS

THR

LEU

LYS

GLU

LEU

8

GLY

LEU

VAL

ASP

GLY

GLN

GLU

LEU

ALA

VAL

9

ALA

ASP

VAL

THR

PRO

GLN

THR

VAL

LEU

10

PHE

LYS

LEU

HIS

PHE

THR

SER

Samples:

sample_1: Uba3, [U-100% 13C; U-100% 15N], 0.4 mM; sodium phosphate 20 mM; sodium chloride 100 mM; sodium azide 0.02%; sodium azide 90%; sodium azide 10%

sample_conditions_1: ionic strength: 127 mM; pH: 6.0; pressure: 1 atm; temperature: 298 K

Experiments:

Name	Sample	Sample state	Sample conditions
3D 1H-15N NOESY	sample_1	isotropic	sample_conditions_1
3D 1H-13C NOESY aliphatic	sample_1	isotropic	sample_conditions_1
3D 1H-13C NOESY aromatic	sample_1	isotropic	sample_conditions_1

Software:

CYANA, Guntert, Mumenthaler and Wuthrich - refinement, structure solution

XEASY, Bartels et al. - chemical shift assignment

X-PLOR NIH, Schwieters, Kuszewski, Tjandra and Clore - refinement

TOPSPIN, Bruker Biospin - collection

NMR spectrometers:

Bruker Avance 600 MHz

PDB	1R4M 1R4N 1TT5 1Y8X 1YOV 2LQ7 2NVU 3DBH 3DBL 3DBR 3FN1 3GZN
DBJ	BAA33144 BAC27905 BAC33258 BAE35036 BAE38920
EMBL	CAB55996 CAG38568 CAH90059 CAH92113 CAH92482
GB	AAC27323 AAC27648 AAH02002 AAH22853 AAH80776
REF	NP_001069042 NP_001104576 NP_001126234 NP_001128861 NP_001252890
SP	Q5R4A0 Q8C878 Q8TBC4 Q99MI7
TPG	DAA17089
AlphaFold	Q5R4A0 Q8C878 Q8TBC4 Q99MI7