BMRB Entry 18288

Name: 1H, 13C and 15N chemical shift assignments of Ninjurin1 ENT domain
Published: 2012-03-22

Chem Shift validation: AVS_full, LACS
BMRB Entry DOI: doi:10.13018/BMR18288

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Nicholas Rego and David Koes
3Dmol.js: molecular visualization with WebGL
Bioinformatics (2015) 31 (8): 1322-1324 doi:10.1093/bioinformatics/btu829

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Title:

1H, 13C and 15N chemical shift assignments of Ninjurin1 ENT domain

Deposition date:

2012-02-21

Original release date:

2012-03-22

Authors:

Jang, Sunbok; Kim, Jihun; Lee, Kiyoung; Lee, Kyuyeun

Citation:

Citation: Lee, In-Gyun; Jang, Sun-Bok; Kim, Ji-Hun; Lee, Ki-Young; Lee, Kyu-Yeon; Cheong, Hae-Kap; Lee, Bong-Jin. "1H, 13C and 15N chemical shift assignments of Ninjurin1 Extracellular N-terminal Domain." Biomol. NMR Assignments 7, 159-162 (2013).
PubMed: 22696136

Assembly members:

Assembly members:
Ninjurin1, polymer, 89 residues, Formula weight is not available

Natural source:

Natural source: Common Name: Human Taxonomy ID: 9606 Superkingdom: Eukaryota Kingdom: Metazoa Genus/species: Homo sapiens

Experimental source:

Experimental source: Production method: recombinant technology Host organism: Escherichia coli Vector: pET21a

Entity Sequences (FASTA):

Entity Sequences (FASTA):
Ninjurin1: MDSGTEEYELNGGLPPGTPG SPDASPARWGWRHGPINVNH YASKKSAAESMLDIALLMAN ASQLKAVVEQGPSFAFYVPL VLEHHHHHH

Data sets:

assigned_chemical_shifts
- assigned_chem_shift_list_1

Data type	Count
13C chemical shifts	233
15N chemical shifts	73
1H chemical shifts	248

Additional metadata:

Assembly
Samples and Experiments
Software
Spectrometers
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Assembly:

Entity Assembly ID	Entity Name	Entity ID
1	Ninjurin1	1

Entities:

Entity 1, Ninjurin1 89 residues - Formula weight is not available

1

MET

ASP

SER

GLY

THR

GLU

TYR

GLU

LEU

2

ASN

GLY

LEU

PRO

GLY

THR

PRO

GLY

3

SER

PRO

ASP

ALA

SER

PRO

ALA

ARG

TRP

GLY

4

TRP

ARG

HIS

GLY

PRO

ILE

ASN

VAL

ASN

HIS

5

TYR

ALA

SER

LYS

SER

ALA

GLU

SER

6

MET

LEU

ASP

ILE

ALA

LEU

MET

ALA

ASN

7

ALA

SER

GLN

LEU

LYS

ALA

VAL

GLU

GLN

8

GLY

PRO

SER

PHE

ALA

PHE

TYR

VAL

PRO

LEU

9

VAL

LEU

GLU

HIS

Samples:

sample_1: Ninjurin1, [U-100% 13C; U-100% 15N], 1 mM; sodium phosphate 20 mM; sodium chloride 50 mM; EDTA 1 mM; PMSF 0.1 mM; DTT 1 mM; SDS, [U-100% 13C], 200 mM

sample_conditions_1: ionic strength: 50 mM; pH: 7; pressure: 1 atm; temperature: 303 K

Experiments:

Name	Sample	Sample state	Sample conditions
2D 1H-15N HSQC	sample_1	isotropic	sample_conditions_1
3D HNCA	sample_1	isotropic	sample_conditions_1
3D HN(CO)CA	sample_1	isotropic	sample_conditions_1
3D HNCO	sample_1	isotropic	sample_conditions_1
3D HCACO	sample_1	isotropic	sample_conditions_1
3D CBCA(CO)NH	sample_1	isotropic	sample_conditions_1
3D HNCACB	sample_1	isotropic	sample_conditions_1
3D 1H-15N TOCSY	sample_1	isotropic	sample_conditions_1
3D HBHA(CO)NH	sample_1	isotropic	sample_conditions_1

Software:

NMRView, Johnson, One Moon Scientific - chemical shift assignment

TALOS, Cornilescu, Delaglio and Bax - structure solution

NMRDraw, Delaglio, Grzesiek, Vuister, Zhu, Pfeifer and Bax - processing

NMRPipe, Delaglio, Grzesiek, Vuister, Zhu, Pfeifer and Bax - processing

NMR spectrometers:

Bruker Avance 900 MHz
Bruker Avance 600 MHz

GB	AAB17560 AAC14593 AAH00298 AAH04440 AAH19336
REF	NP_004139 XP_002820014 XP_004048327 XP_004048328 XP_004048329
SP	Q92982
AlphaFold	Q92982