BMRB Entry 18276

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PDB ID:
Entry in NMR Restraints Grid
Validation report in NRG-CING
Chem Shift validation: AVS_anomalous, AVS_full
BMRB Entry DOI: doi:10.13018/BMR18276
MolProbity Validation Chart

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Title:
1H, 13C and 15N chemical shifts for Salmonella Typhimurium PrgI Type three secretion system needle subunit
Deposition date:
2012-02-18
Original release date:
2012-03-23
Authors:
Loquet, Antoine; Lv, Guohua; Giller, Karin; Becker, Stefan; Lange, Adam
Citation:

Citation: Loquet, Antoine; Lv, Guohua; Giller, Karin; Becker, Stefan; Lange, Adam. "13C spin dilution for simplified and complete solid-state NMR resonance assignment of insoluble biological assemblies"  J. Am. Chem. Soc. 133, 4722-4725 (2011).
PubMed: 21401039

Assembly members:

Assembly members:
PrgI, polymer, 80 residues, Formula weight is not available

Natural source:

Natural source:   Common Name: Salmonella enterica   Taxonomy ID: 28901   Superkingdom: Bacteria   Kingdom: not available   Genus/species: Salmonella Salmonella enterica

Experimental source:

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli   Vector: BL21 (DE3)

Entity Sequences (FASTA):

Entity Sequences (FASTA):
PrgI: MATPWSGYLDDVSAKFDTGV DNLQTQVTEALDKLAAKPSD PALLAAYQSKLSEYNLYRNA QSNTVKVFKDIDAAIIQNFR

Data sets:
Data typeCount
13C chemical shifts380
15N chemical shifts85
1H chemical shifts74

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
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Assembly:

Entity Assembly IDEntity NameEntity ID
1PrgI needle1

Entities:

Entity 1, PrgI needle 80 residues - Formula weight is not available

1   METALATHRPROTRPSERGLYTYRLEUASP
2   ASPVALSERALALYSPHEASPTHRGLYVAL
3   ASPASNLEUGLNTHRGLNVALTHRGLUALA
4   LEUASPLYSLEUALAALALYSPROSERASP
5   PROALALEULEUALAALATYRGLNSERLYS
6   LEUSERGLUTYRASNLEUTYRARGASNALA
7   GLNSERASNTHRVALLYSVALPHELYSASP
8   ILEASPALAALAILEILEGLNASNPHEARG

Samples:

sample_1: PrgI, [U-100% 13C; U-100% 15N], 10 mg

sample_conditions_1: pH: 5.5; pressure: 1 atm; temperature: 278 K

Experiments:

NameSampleSample stateSample conditions
13C-13C (PDSD)sample_1solidsample_conditions_1
15N-13C (Specific-CP)sample_1solidsample_conditions_1

Software:

SPARKY, Goddard - data analysis

NMR spectrometers:

  • Bruker Avance 850 MHz

Related Database Links:

BMRB 16770
PDB
DBJ BAJ37865 BAP08778
EMBL CAR34293 CAR38585 CBG25842 CBW18951 CBY97043
GB AAB60189 AAL21753 AAX49613 AAX66711 ABX68915
REF NP_461794 WP_000235228 WP_001143113 WP_001527993 WP_023259990
SP P41784
AlphaFold P41784

Download HSQC peak lists in one of the following formats:
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