BMRB Entry 18268

Name: Unliganded (apo) C-terminal EF-hand domain from human polycystin-2
Published: 2012-05-08

Chem Shift validation: AVS_full, LACS
BMRB Entry DOI: doi:10.13018/BMR18268

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Nicholas Rego and David Koes
3Dmol.js: molecular visualization with WebGL
Bioinformatics (2015) 31 (8): 1322-1324 doi:10.1093/bioinformatics/btu829

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Title:

Unliganded (apo) C-terminal EF-hand domain from human polycystin-2

Deposition date:

2012-02-15

Original release date:

2012-05-08

Authors:

Petri, Edward; Celic, Andjelka; Ehrlich, Barbara; Boggon, Titus; Hodsdon, Michael

Citation:

Citation: Celic, Andjelka; Petri, Edward; Benbow, Jennifer; Hodsdon, Michael; Ehrlich, Barbara; Boggon, Titus. "Calcium-induced Conformational Changes in C-terminal Tail of Polycystin-2 Are Necessary for Channel Gating." J. Biol. Chem. 287, 17232-17240 (2012).
PubMed: 22474326

Assembly members:

Assembly members:
EF-hand_domain_of_polycystin-2, polymer, 78 residues, Formula weight is not available

Natural source:

Natural source: Common Name: Humans Taxonomy ID: 9606 Superkingdom: Eukaryota Kingdom: Metazoa Genus/species: Homo sapiens

Experimental source:

Experimental source: Production method: recombinant technology Host organism: Escherichia coli Vector: pET-28(a+)

Entity Sequences (FASTA):

Entity Sequences (FASTA):
EF-hand_domain_of_polycystin-2: NTVDDISESLRQGGGKLNFD ELRQDLKGKGHTDAEIEAIF TKYDQDGDQELTEHEHQQMR DDLEKEREDLDLDHSSLP

Data sets:

assigned_chemical_shifts
- assigned_chem_shift_list_1

Data type	Count
13C chemical shifts	145
15N chemical shifts	72
1H chemical shifts	163

Additional metadata:

Assembly
Samples and Experiments
Software
Spectrometers
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Assembly:

Entity Assembly ID	Entity Name	Entity ID
1	EF-hand_domain_of_polycystin-2	1

Entities:

Entity 1, EF-hand_domain_of_polycystin-2 78 residues - Formula weight is not available

1

ASN

THR

VAL

ASP

ILE

SER

GLU

SER

LEU

2

ARG

GLN

GLY

LYS

LEU

ASN

PHE

ASP

3

GLU

LEU

ARG

GLN

ASP

LEU

LYS

GLY

LYS

GLY

4

HIS

THR

ASP

ALA

GLU

ILE

GLU

ALA

ILE

PHE

5

THR

LYS

TYR

ASP

GLN

ASP

GLY

ASP

GLN

GLU

6

LEU

THR

GLU

HIS

GLU

HIS

GLN

MET

ARG

7

ASP

LEU

GLU

LYS

GLU

ARG

GLU

ASP

LEU

8

ASP

LEU

ASP

HIS

SER

LEU

PRO

Samples:

sample_1: EF-hand_domain_of_polycystin-2, [U-100% 13C; U-100% 15N], 1.0 mM; H2O 90%; D2O, [U-2H], 10%

sample_conditions_1: ionic strength: 0.15 M; pH: 7.4; pressure: 1 atm; temperature: 298 K

Experiments:

Name	Sample	Sample state	Sample conditions
2D 1H-15N HSQC	sample_1	isotropic	sample_conditions_1
3D HNCACB	sample_1	isotropic	sample_conditions_1
3D 1H-15N NOESY	sample_1	isotropic	sample_conditions_1

Software:

NMRPipe, Delaglio, Grzesiek, Vuister, Zhu, Pfeifer and Bax - processing

VNMR v6.2c, Varian - collection

SPARKY, Goddard - chemical shift assignment, peak picking

NMR spectrometers:

Varian INOVA 600 MHz

BMRB	16191 16590 17621
PDB	2KLD 2KLE 2KQ6 2Y4Q
DBJ	BAG56956 BAG57494
EMBL	CAG31243 CAI38797
GB	AAC16004 AAC50520 AAC50933 AAI11455 AAI12262
REF	NP_000288 NP_001026311 NP_001039777 NP_001232908 XP_001099242
SP	Q13563 Q4GZT3
TPG	DAA28806
AlphaFold	Q13563 Q4GZT3