BMRB Entry 18268

Chem Shift validation: AVS_full, LACS
BMRB Entry DOI: doi:10.13018/BMR18268

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Title:
Unliganded (apo) C-terminal EF-hand domain from human polycystin-2
Deposition date:
2012-02-15
Original release date:
2012-05-08
Authors:
Petri, Edward; Celic, Andjelka; Ehrlich, Barbara; Boggon, Titus; Hodsdon, Michael
Citation:

Citation: Celic, Andjelka; Petri, Edward; Benbow, Jennifer; Hodsdon, Michael; Ehrlich, Barbara; Boggon, Titus. "Calcium-induced Conformational Changes in C-terminal Tail of Polycystin-2 Are Necessary for Channel Gating."  J. Biol. Chem. 287, 17232-17240 (2012).
PubMed: 22474326

Assembly members:

Assembly members:
EF-hand_domain_of_polycystin-2, polymer, 78 residues, Formula weight is not available

Natural source:

Natural source:   Common Name: Humans   Taxonomy ID: 9606   Superkingdom: Eukaryota   Kingdom: Metazoa   Genus/species: Homo sapiens

Experimental source:

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli   Vector: pET-28(a+)

Entity Sequences (FASTA):

Entity Sequences (FASTA):
EF-hand_domain_of_polycystin-2: NTVDDISESLRQGGGKLNFD ELRQDLKGKGHTDAEIEAIF TKYDQDGDQELTEHEHQQMR DDLEKEREDLDLDHSSLP

Data sets:
Data typeCount
13C chemical shifts145
15N chemical shifts72
1H chemical shifts163

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
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Assembly:

Entity Assembly IDEntity NameEntity ID
1EF-hand_domain_of_polycystin-21

Entities:

Entity 1, EF-hand_domain_of_polycystin-2 78 residues - Formula weight is not available

1   ASNTHRVALASPASPILESERGLUSERLEU
2   ARGGLNGLYGLYGLYLYSLEUASNPHEASP
3   GLULEUARGGLNASPLEULYSGLYLYSGLY
4   HISTHRASPALAGLUILEGLUALAILEPHE
5   THRLYSTYRASPGLNASPGLYASPGLNGLU
6   LEUTHRGLUHISGLUHISGLNGLNMETARG
7   ASPASPLEUGLULYSGLUARGGLUASPLEU
8   ASPLEUASPHISSERSERLEUPRO

Related Database Links:

BMRB 16191 16590 17621
PDB 2KLD 2KLE 2KQ6 2Y4Q
DBJ BAG56956 BAG57494
EMBL CAG31243 CAI38797
GB AAC16004 AAC50520 AAC50933 AAI11455 AAI12262
REF NP_000288 NP_001026311 NP_001039777 NP_001232908 XP_001099242
SP Q13563 Q4GZT3
TPG DAA28806
AlphaFold Q13563 Q4GZT3

Download HSQC peak lists in one of the following formats:
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SPARKY: Backbone or all simulated peaks