BMRB Entry 18253

Chem Shift validation: AVS_full, LACS
BMRB Entry DOI: doi:10.13018/BMR18253

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Title:
1H, 13C and 15N NMR assignments of inactive form of P1 endolysin Lyz at pH 7.5
Deposition date:
2012-02-10
Original release date:
2012-03-23
Authors:
Kashyap, Maruthi; Bhavesh, Neel Sarovar; Arulandu, Arockiasamy; Krishna Das, Bhaba; Jagga, Zeenia
Citation:

Citation: Kashyap, Maruthi; Jagga, Zeenia; Das, Bhaba Krishna; Arockiasamy, Arulandu; Bhavesh, Neel Sarovar. "(1)H, (13)C and (15)N NMR assignments of inactive form of P1 endolysin Lyz."  Biomol. NMR Assignments 6, 87-89 (2012).
PubMed: 21822941

Assembly members:

Assembly members:
P1_endolysin_Lyz, polymer, 163 residues, Formula weight is not available

Natural source:

Natural source:   Common Name: P1 Bacteriophage   Taxonomy ID: not available   Superkingdom: viruses   Kingdom: not available   Genus/species: P1 Bacteriophage

Experimental source:

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli   Vector: pET28a

Entity Sequences (FASTA):

Entity Sequences (FASTA):
P1_endolysin_Lyz: RTNQAGLELIGNAEGCRRDP YMCPAGVWTDGIGNTHGVTP GVRKTDQQIAADWEKNILIA ERCINQHFRGKDMPDNAFSA MTSAAFNMGCNSLRTYYSKA RGMRVETSIHKWAQKGEWVN MCNHLPDFVNSNGVPLRGLK IRREKERQLCLTGLVNEHHH HHH

Data sets:
Data typeCount
13C chemical shifts608
15N chemical shifts176
1H chemical shifts777

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1P1 endolysin Lyz1

Entities:

Entity 1, P1 endolysin Lyz 163 residues - Formula weight is not available

1   ARGTHRASNGLNALAGLYLEUGLULEUILE
2   GLYASNALAGLUGLYCYSARGARGASPPRO
3   TYRMETCYSPROALAGLYVALTRPTHRASP
4   GLYILEGLYASNTHRHISGLYVALTHRPRO
5   GLYVALARGLYSTHRASPGLNGLNILEALA
6   ALAASPTRPGLULYSASNILELEUILEALA
7   GLUARGCYSILEASNGLNHISPHEARGGLY
8   LYSASPMETPROASPASNALAPHESERALA
9   METTHRSERALAALAPHEASNMETGLYCYS
10   ASNSERLEUARGTHRTYRTYRSERLYSALA
11   ARGGLYMETARGVALGLUTHRSERILEHIS
12   LYSTRPALAGLNLYSGLYGLUTRPVALASN
13   METCYSASNHISLEUPROASPPHEVALASN
14   SERASNGLYVALPROLEUARGGLYLEULYS
15   ILEARGARGGLULYSGLUARGGLNLEUCYS
16   LEUTHRGLYLEUVALASNGLUHISHISHIS
17   HISHISHIS

Samples:

sample_1: P1 endolysin Lyz, [U-13C; U-15N], 1 mM; H2O 90%; D2O 10%

sample_conditions_1: ionic strength: 50 mM; pH: 7.5; pressure: 1 atm; temperature: 298 K

Experiments:

NameSampleSample stateSample conditions
2D 1H-15N HSQCsample_1isotropicsample_conditions_1
2D 1H-13C HSQC aliphaticsample_1isotropicsample_conditions_1
2D 1H-13C HSQC aromaticsample_1isotropicsample_conditions_1
3D HNCAsample_1isotropicsample_conditions_1
3D HNCACBsample_1isotropicsample_conditions_1
3D 1H-15N NOESYsample_1isotropicsample_conditions_1
3D HNCOsample_1isotropicsample_conditions_1

Software:

TOPSPIN v2.1, Bruker Biospin, Keller and Wuthrich - chemical shift assignment, processing

NMR spectrometers:

  • Bruker Avance 700 MHz

Related Database Links:

BMRB 17656
PDB
DBJ BAI39214
EMBL CAA61013 CCP96781 CDK52642 CDK58555 CDK88832
GB AAD20630 AAQ07493 AAQ13989 AAQ14097 AEJ60171
REF WP_000299977 WP_000676956 WP_001339174 WP_001345478 WP_001372701
SP Q37875
AlphaFold Q37875

Download HSQC peak lists in one of the following formats:
CSV: Backbone or all simulated peaks
SPARKY: Backbone or all simulated peaks