BMRB Entry 18248

Name: Assignments for the dually targeting peptide from Thr-tRNA synthetase
Published: 2012-08-27

Chem Shift validation: AVS_full
BMRB Entry DOI: doi:10.13018/BMR18248

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Nicholas Rego and David Koes
3Dmol.js: molecular visualization with WebGL
Bioinformatics (2015) 31 (8): 1322-1324 doi:10.1093/bioinformatics/btu829

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Title:

Assignments for the dually targeting peptide from Thr-tRNA synthetase

Deposition date:

2012-02-09

Original release date:

2012-08-27

Authors:

Ye, Weihua; Spanning, Erika; Unnerstale, Sofia; Gotthold, David; Glaser, Elzbieta; Maler, Lena

Citation:

Citation: Ye, Weihua; Spanning, Erika; Unnerstale, Sofia; Gotthold, David; Glaser, Elzbieta; Maler, Lena. "NMR investigations of the dual targeting peptide of Thr-tRNA synthetase and its interaction with the mitochondrial Tom20 receptor in Arabidopsisthaliana." FEBS J. 279, 3738-3748 (2012).
PubMed: 22863398

Assembly members:

Assembly members:
ThrRS-dTP(2-60), polymer, 59 residues, Formula weight is not available

Natural source:

Natural source: Common Name: Thale cress Taxonomy ID: 3702 Superkingdom: Eukaryota Kingdom: Viridiplantae Genus/species: Arabidopsis thaliana

Experimental source:

Experimental source: Production method: recombinant technology Host organism: Escherichia coli Vector: pGEX-6p-2

Entity Sequences (FASTA):

Entity Sequences (FASTA):
ThrRS-dTP(2-60): ASSHSLLFSSSFLSKPSSFT SSLRRFVYLPTRQFWPRQRH GFSTVFAVATEPAISSSGP

Data sets:

assigned_chemical_shifts
- assigned_chem_shift_list_1

Data type	Count
13C chemical shifts	49
15N chemical shifts	51
1H chemical shifts	277

Additional metadata:

Assembly
Samples and Experiments
Software
Spectrometers
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Assembly:

Entity Assembly ID	Entity Name	Entity ID
1	ThrRS-dTP	1

Entities:

Entity 1, ThrRS-dTP 59 residues - Formula weight is not available

1

ALA

SER

HIS

SER

LEU

PHE

SER

2

SER

PHE

LEU

SER

LYS

PRO

SER

PHE

THR

3

SER

LEU

ARG

PHE

VAL

TYR

LEU

PRO

4

THR

ARG

GLN

PHE

TRP

PRO

ARG

GLN

ARG

HIS

5

GLY

PHE

SER

THR

VAL

PHE

ALA

VAL

ALA

THR

6

GLU

PRO

ALA

ILE

SER

GLY

PRO

Samples:

sample_1: ThrRS-dTP(2-60), [U-15N], 80 – 100 uM; D2O, [U-100% 2H], 10%; sodium phosphate 50 mM

sample_2: ThrRS-dTP(2-60), [U-13C; U-15N], 80 – 100 uM; D2O, [U-100% 2H], 10%; sodium phosphate 50 mM

sample_conditions_1: pH: 6.0; temperature: 298 K

sample_conditions_2: pH: 6.0; temperature: 298 K

Experiments:

Name	Sample	Sample state	Sample conditions
2D 1H-15N HSQC	sample_1	isotropic	sample_conditions_1
3D 1H-15N NOESY	sample_1	isotropic	sample_conditions_1
3D 1H-15N TOCSY	sample_1	isotropic	sample_conditions_1
HNCA	sample_2	isotropic	sample_conditions_2
HN(CO)CA	sample_2	isotropic	sample_conditions_2

Software:

SPARKY, Goddard - chemical shift assignment

NMR spectrometers:

Bruker Avance 600 MHz
Bruker Avance 700 MHz

GB	AEC05873
REF	NP_671778
SP	F4IFC5
AlphaFold	F4IFC5