BMRB Entry 18201

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PDB ID:
Entry in NMR Restraints Grid
Validation report in NRG-CING
Chem Shift validation: AVS_full, LACS
BMRB Entry DOI: doi:10.13018/BMR18201
MolProbity Validation Chart

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Title:
1H, 13C and 15N resonance assignments of the periplasmic signaling domain of HasR, a TonB-dependent outer membrane heme transporter
Deposition date:
2012-01-18
Original release date:
2012-03-23
Authors:
Malki, Idir; Cardoso de Amorim, Gisele; Simenel, Catherine; Prochnicka-Chalufour, Ada; Delepierre, Muriel; Izadi-Pruneyre, Nadia
Citation:

Citation: Malki, Idir; Cardoso de Amorim, Gisele; Simenel, Catherine; Prochnicka-Chalufour, Ada; Delepierre, Muriel; Izadi-Pruneyre, Nadia. "(1)H, (13)C and (15)N resonance assignments of the periplasmic signalling domain of HasR, a TonB-dependent outer membrane heme transporter."  Biomol. NMR Assignments 7, 43-46 (2013).
PubMed: 22415545

Assembly members:

Assembly members:
HasR_N-terminal_periplasmic_signaling_domain, polymer, 107 residues, Formula weight is not available

Natural source:

Natural source:   Common Name: Serratia marcescens   Taxonomy ID: 615   Superkingdom: Bacteria   Kingdom: not available   Genus/species: Serratia marcescens

Experimental source:

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli   Vector: pET41

Entity Sequences (FASTA):

Entity Sequences (FASTA):
HasR_N-terminal_periplasmic_signaling_domain: SMAQAAQQKNFNIAAQPLQS AMLRFAEQAGMQVFFDEVKL DGMQAAALNGSMSVEQGLRR LIGGNPVAFRLQPQGQIVLS RLPTANGDGGALALDSLTVL GAGGNNA

Data sets:
Data typeCount
13C chemical shifts403
15N chemical shifts107
1H chemical shifts657

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
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Assembly:

Entity Assembly IDEntity NameEntity ID
1HasR N-terminal periplasmic signaling domain1

Entities:

Entity 1, HasR N-terminal periplasmic signaling domain 107 residues - Formula weight is not available

1   SERMETALAGLNALAALAGLNGLNLYSASN
2   PHEASNILEALAALAGLNPROLEUGLNSER
3   ALAMETLEUARGPHEALAGLUGLNALAGLY
4   METGLNVALPHEPHEASPGLUVALLYSLEU
5   ASPGLYMETGLNALAALAALALEUASNGLY
6   SERMETSERVALGLUGLNGLYLEUARGARG
7   LEUILEGLYGLYASNPROVALALAPHEARG
8   LEUGLNPROGLNGLYGLNILEVALLEUSER
9   ARGLEUPROTHRALAASNGLYASPGLYGLY
10   ALALEUALALEUASPSERLEUTHRVALLEU
11   GLYALAGLYGLYASNASNALA

Samples:

sample_1: HasR_N-terminal_periplasmic_signaling_domain, [U-99% 13C; U-99% 15N], 0.5 mM; Sodium phosphate 50 mM; NaCl 50 mM

sample_conditions_1: ionic strength: 0.1 M; pH: 7.0; pressure: 1 atm; temperature: 293 K

Experiments:

NameSampleSample stateSample conditions
2D 1H-15N HSQCsample_1isotropicsample_conditions_1
2D 1H-13C HSQCsample_1isotropicsample_conditions_1
3D CBCA(CO)NHsample_1isotropicsample_conditions_1
3D HNCACBsample_1isotropicsample_conditions_1
3D HNCOsample_1isotropicsample_conditions_1
3D C(CO)NHsample_1isotropicsample_conditions_1
3D H(CCO)NHsample_1isotropicsample_conditions_1
3D HCCH-TOCSYsample_1isotropicsample_conditions_1
2D HbCbCgCdHdsample_1isotropicsample_conditions_1
2D HbCbCgCdCeHesample_1isotropicsample_conditions_1

Software:

ANALYSIS v2.15, CCPN - chemical shift assignment

NMR spectrometers:

  • Varian NMR System 600 MHz

Related Database Links:

PDB
DBJ BAO32406
EMBL CAE46936 CDG10875
GB ALL36750 EMF03485 ERH72206 ETX40680 KFD10590
REF WP_004940484 WP_016928899 WP_019454232 WP_021504699 WP_025301725

Download HSQC peak lists in one of the following formats:
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