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PDB ID:
Entry in NMR Restraints Grid
Validation report in NRG-CING
Chem Shift validation: AVS_full
BMRB Entry DOI: doi:10.13018/BMR18193
MolProbity Validation Chart
NMR-STAR file interactive viewer.
NMR-STAR v3 text file.
XML gzip file.
RDF gzip file.
All files associated with the entry
Citation: Gallego-Garcia, Aranzazu; Mirassou, Yasmina; Elias-Arnanz, Monserrat; Padmanabhan, S.; Jimenez, Angeles. "NMR structure note: N-terminal domain of Thermus thermophilus CdnL" J. Biomol. NMR 53, 355-363 (2012).
PubMed: 22782235
Assembly members:
TtCdnLNt, polymer, 70 residues, 7437.5 Da.
Natural source: Common Name: Thermus thermophilus Taxonomy ID: 274 Superkingdom: Bacteria Kingdom: not available Genus/species: Thermus thermophilus
Experimental source: Production method: recombinant technology Host organism: Escherichia coli Vector: pTYB12
Entity Sequences (FASTA):
TtCdnLNt: AGHMKEFRPGDKVVLPPYGV
GVVAGIAQRSVSGVSRAYYQ
VDFPGSRSKAYVPVEAPHSV
GLRKALAPEE
Data type | Count |
13C chemical shifts | 278 |
15N chemical shifts | 172 |
1H chemical shifts | 587 |
Entity Assembly ID | Entity Name | Entity ID |
---|---|---|
1 | TtCdnL | 1 |
Entity 1, TtCdnL 70 residues - 7437.5 Da.
AGH is the N-terminal cloning tag
1 | ALA | GLY | HIS | MET | LYS | GLU | PHE | ARG | PRO | GLY | |
2 | ASP | LYS | VAL | VAL | LEU | PRO | PRO | TYR | GLY | VAL | |
3 | GLY | VAL | VAL | ALA | GLY | ILE | ALA | GLN | ARG | SER | |
4 | VAL | SER | GLY | VAL | SER | ARG | ALA | TYR | TYR | GLN | |
5 | VAL | ASP | PHE | PRO | GLY | SER | ARG | SER | LYS | ALA | |
6 | TYR | VAL | PRO | VAL | GLU | ALA | PRO | HIS | SER | VAL | |
7 | GLY | LEU | ARG | LYS | ALA | LEU | ALA | PRO | GLU | GLU |
sample_1: TtCdnLNt 1 mM; sodium phosphate 50 mM; sodium chloride 100 mM; sodium azide 0.05%; DSS 0.5 mM; H2O 90%; D2O 10%
sample_2: TtCdnLNt 1 mM; sodium phosphate 50 mM; sodium chloride 100 mM; sodium azide 0.05%; DSS 0.5 mM; D2O 100%
sample_3: TtCdnLNt, [U-100% 13C; U-100% 15N], 1 mM; sodium phosphate 50 mM; sodium chloride 100 mM; sodium azide 0.05%; DSS 0.5 mM; H2O 90%; D2O 10%
sample_4: TtCdnLNt, [U-100% 13C; U-100% 15N], 1 mM; sodium phosphate 50 mM; sodium chloride 100 mM; sodium azide 0.05%; DSS 0.5 mM; D2O 100%
sample_conditions_1: ionic strength: 0.15 M; pH: 7.0; pressure: 1 atm; temperature: 298 K
sample_conditions_2: ionic strength: 0.15 M; pH: 7.0; pressure: 1 atm; temperature: 288 K
sample_conditions_3: ionic strength: 0.15 M; pH: 7.0; pressure: 1 atm; temperature: 308 K
Name | Sample | Sample state | Sample conditions |
---|---|---|---|
2D 1H-1H COSY | sample_1 | isotropic | sample_conditions_1 |
2D 1H-1H TOCSY | sample_1 | isotropic | sample_conditions_1 |
2D 1H-1H NOESY | sample_1 | isotropic | sample_conditions_1 |
2D 1H-1H COSY | sample_2 | isotropic | sample_conditions_1 |
2D 1H-1H TOCSY | sample_2 | isotropic | sample_conditions_1 |
2D 1H-1H NOESY | sample_2 | isotropic | sample_conditions_1 |
2D 1H-15N HSQC | sample_3 | isotropic | sample_conditions_1 |
3D HNCO | sample_3 | isotropic | sample_conditions_1 |
3D HNCA | sample_3 | isotropic | sample_conditions_1 |
3D CBCA(CO)NH | sample_3 | isotropic | sample_conditions_1 |
3D HNCACB | sample_3 | isotropic | sample_conditions_1 |
3D HBHA(CO)NH | sample_3 | isotropic | sample_conditions_1 |
3D HBHANH | sample_3 | isotropic | sample_conditions_1 |
2D 1H-13C HSQC aliphatic | sample_4 | isotropic | sample_conditions_1 |
2D 1H-13C HSQC aromatic | sample_4 | isotropic | sample_conditions_1 |
3D HCCH-TOCSY | sample_4 | isotropic | sample_conditions_1 |
3D 1H-13C NOESY | sample_4 | isotropic | sample_conditions_1 |
2D 1H-15N HSQC | sample_3 | isotropic | sample_conditions_2 |
2D 1H-15N HSQC | sample_3 | isotropic | sample_conditions_3 |
TOPSPIN, Bruker Biospin - collection, processing
SPARKY, Goddard - chemical shift assignment, peak picking
PDB | |
DBJ | BAD69991 |
GB | AAS82160 AEG32654 AFH38154 EIA39918 KHG65550 |
REF | WP_008630850 WP_011174173 WP_011227752 WP_019551571 WP_022798969 |
Download HSQC peak lists in one of the following formats:
CSV: Backbone
or all simulated peaks
SPARKY: Backbone
or all simulated peaks