BMRB Entry 18189

Title:
DocA
Deposition date:
2012-01-09
Original release date:
2012-03-27
Authors:
Feng, Yingang; Cui, Zhenling; Li, Yifei; Cui, Qiu
Citation:

Citation: Cui, Zhenling; Li, Yifei; Xiao, Yan; Feng, Yingang; Cui, Qui. "Resonance assignments of cohesin and dockerin domains from Clostridium acetobutylicum ATCC824."  Biomol. NMR Assignments 7, 73-76 (2013).
PubMed: 22446850

Assembly members:

Assembly members:
DocA, polymer, 67 residues, Formula weight is not available

Natural source:

Natural source:   Common Name: Clostridium acetobutylicum   Taxonomy ID: 1488   Superkingdom: Bacteria   Kingdom: not available   Genus/species: Clostridium acetobutylicum

Experimental source:

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli   Vector: pET28aNS

Entity Sequences (FASTA):

Data sets:
Data typeCount
13C chemical shifts266
15N chemical shifts76
1H chemical shifts443

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1DocA1

Entities:

Entity 1, DocA 67 residues - Formula weight is not available

1   GLYSERALASERASNTHRILELEUGLYASP
2   LEUASNASPASPGLYVALVALASNGLYARG
3   ASPILEVALMETMETARGGLNTYRLEUALA
4   GLYLYSTHRVALSERGLYILEASPLYSASN
5   ALALEUASPILEASNGLYASPGLYALAVAL
6   ASNGLYARGASPLEUMETGLULEUILELYS
7   LYSVALSERASNASNTHRSER

Samples:

sample_1: DocA, [U-100% 13C; U-100% 15N], 0.6 mM; sodium acetate 20 mM; potassium chloride 100 mM; calcium chloride 1 mM; sodium azide 0.01 % w/v; DSS 0.05 % w/v

sample_conditions_1: ionic strength: 121 mM; pH: 4.8; pressure: 1 atm; temperature: 298 K

Experiments:

NameSampleSample stateSample conditions
2D 1H-15N HSQCsample_1isotropicsample_conditions_1
2D 1H-13C HSQCsample_1isotropicsample_conditions_1
3D HNCACBsample_1isotropicsample_conditions_1
3D CBCA(CO)NHsample_1isotropicsample_conditions_1
3D HNCOsample_1isotropicsample_conditions_1
3D HBHA(CO)NHsample_1isotropicsample_conditions_1
3D HCCH-TOCSYsample_1isotropicsample_conditions_1
3D 1H-15N NOESYsample_1isotropicsample_conditions_1
3D 1H-13C NOESYsample_1isotropicsample_conditions_1
3D 1H-13C NOESY aromaticsample_1isotropicsample_conditions_1

Software:

NMRView, Johnson, One Moon Scientific - chemical shift assignment, peak picking

NMRPipe, Delaglio, Grzesiek, Vuister, Zhu, Pfeifer and Bax - data analysis, processing

NMR spectrometers:

  • Bruker DMX 600 MHz

Related Database Links:

GB AAK78893 ADZ19968 AEI31499 KHD35937
REF NP_347553 WP_010964235 WP_034584222

Download HSQC peak lists in one of the following formats:
CSV: Backbone or all simulated peaks
SPARKY: Backbone or all simulated peaks