BMRB Entry 18188

Name: CohA2
Published: 2012-03-27

Chem Shift validation: AVS_full, LACS
BMRB Entry DOI: doi:10.13018/BMR18188

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Nicholas Rego and David Koes
3Dmol.js: molecular visualization with WebGL
Bioinformatics (2015) 31 (8): 1322-1324 doi:10.1093/bioinformatics/btu829

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Title:

CohA2

Deposition date:

2012-01-09

Original release date:

2012-03-27

Authors:

Feng, Yingang; Cui, Zhenling; Li, Yifei; Cui, Qiu

Citation:

Citation: Cui, Zhenling; Li, Yifei; Xiao, Yan; Feng, Yingang; Cui, Qui. "Resonance assignments of cohesin and dockerin domains from Clostridium acetobutylicum ATCC824." Biomol. NMR Assignments 7, 73-76 (2013).
PubMed: 22446850

Assembly members:

Assembly members:
CohA2, polymer, 155 residues, Formula weight is not available

Natural source:

Natural source: Common Name: Clostridium acetobutylicum Taxonomy ID: 1488 Superkingdom: Bacteria Kingdom: not available Genus/species: Clostridium acetobutylicum

Experimental source:

Experimental source: Production method: recombinant technology Host organism: Escherichia coli Vector: pET28aNS

Entity Sequences (FASTA):

Entity Sequences (FASTA):
CohA2: GSASVKNETVKLSVGTVSGN PGDTVKVPVTISQVSTPVGL ICMDISYDASKFTVKDVLPN TDLVKDTDNYSFIVNTSTPG KISITFTDPTLANYPISVDG ILAYLDFIINSNATAGDSAL TVDPATLIVADENDKDIKDA ASNGKITVTGSAPTS

Data sets:

assigned_chemical_shifts
- assigned_chem_shift_list_1

Data type	Count
13C chemical shifts	640
15N chemical shifts	151
1H chemical shifts	1024

Additional metadata:

Assembly
Samples and Experiments
Software
Spectrometers
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Assembly:

Entity Assembly ID	Entity Name	Entity ID
1	CohA2	1

Entities:

Entity 1, CohA2 155 residues - Formula weight is not available

1

GLY

SER

ALA

SER

VAL

LYS

ASN

GLU

THR

VAL

2

LYS

LEU

SER

VAL

GLY

THR

VAL

SER

GLY

ASN

3

PRO

GLY

ASP

THR

VAL

LYS

VAL

PRO

VAL

THR

4

ILE

SER

GLN

VAL

SER

THR

PRO

VAL

GLY

LEU

5

ILE

CYS

MET

ASP

ILE

SER

TYR

ASP

ALA

SER

6

LYS

PHE

THR

VAL

LYS

ASP

VAL

LEU

PRO

ASN

7

THR

ASP

LEU

VAL

LYS

ASP

THR

ASP

ASN

TYR

8

SER

PHE

ILE

VAL

ASN

THR

SER

THR

PRO

GLY

9

LYS

ILE

SER

ILE

THR

PHE

THR

ASP

PRO

THR

10

LEU

ALA

ASN

TYR

PRO

ILE

SER

VAL

ASP

GLY

11

ILE

LEU

ALA

TYR

LEU

ASP

PHE

ILE

ASN

12

SER

ASN

ALA

THR

ALA

GLY

ASP

SER

ALA

LEU

13

THR

VAL

ASP

PRO

ALA

THR

LEU

ILE

VAL

ALA

14

ASP

GLU

ASN

ASP

LYS

ASP

ILE

LYS

ASP

ALA

15

ALA

SER

ASN

GLY

LYS

ILE

THR

VAL

THR

GLY

16

SER

ALA

PRO

THR

SER

Samples:

sample_1: CohA2, [U-100% 13C; U-100% 15N], 0.6 mM; sodium acetate 20 mM; potassium chloride 100 mM; calcium chloride 1 mM; sodium azide 0.01 % w/v; DSS 0.05 % w/v

sample_conditions_1: ionic strength: 121 mM; pH: 4.8; pressure: 1 atm; temperature: 298 K

Experiments:

Name	Sample	Sample state	Sample conditions
2D 1H-15N HSQC	sample_1	isotropic	sample_conditions_1
2D 1H-13C HSQC	sample_1	isotropic	sample_conditions_1
3D HNCACB	sample_1	isotropic	sample_conditions_1
3D CBCA(CO)NH	sample_1	isotropic	sample_conditions_1
3D HNCO	sample_1	isotropic	sample_conditions_1
3D HBHA(CO)NH	sample_1	isotropic	sample_conditions_1
3D HCCH-TOCSY	sample_1	isotropic	sample_conditions_1
3D 1H-13C NOESY	sample_1	isotropic	sample_conditions_1
3D 1H-15N NOESY	sample_1	isotropic	sample_conditions_1
3D 1H-13C NOESY aromatic	sample_1	isotropic	sample_conditions_1

Software:

NMRView, Johnson, One Moon Scientific - chemical shift assignment, peak picking

NMRPipe, Delaglio, Grzesiek, Vuister, Zhu, Pfeifer and Bax - data analysis, processing

NMR spectrometers:

Bruker DMX 600 MHz

Download HSQC peak lists in one of the following formats:
CSV: Backbone or all simulated peaks
SPARKY: Backbone or all simulated peaks