BMRB Entry 18187

Title:
Solution structure of the Get5 carboxyl domain from A. fumigatus   PubMed: 22262836
Deposition date:
2012-01-08
Original release date:
2012-02-10
Authors:
Chartron, Justin; VanderVelde, David; Rao, Meera; Clemons, William
Citation:

Citation: Chartron, Justin; Vandervelde, David; Rao, Meera; Clemons, William. "The Get5 carboxyl terminal domain is a novel dimerization motif that tethers an extended Get4/Get5 complex."  J. Biol. Chem. 287, 8310-8317 (2012).

Assembly members:

Assembly members:
Get5, polymer, 75 residues, 5285.887 Da.

Natural source:

Natural source:   Common Name: Aspergillus fumigatus   Taxonomy ID: 746128   Superkingdom: Eukaryota   Kingdom: Metazoa   Genus/species: Aspergillus fumigatus

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli   Vector: pET-33b

Experimental source:

Natural source:   Common Name: Aspergillus fumigatus   Taxonomy ID: 746128   Superkingdom: Eukaryota   Kingdom: Metazoa   Genus/species: Aspergillus fumigatus

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli   Vector: pET-33b

Entity Sequences (FASTA):

Data sets:
Data typeCount
13C chemical shifts321
15N chemical shifts75
1H chemical shifts436

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1Get5, chain 11
2Get5, chain 21

Entities:

Entity 1, Get5, chain 1 75 residues - 5285.887 Da.

1   SERVALASPVALALAVALSERALAGLYALA
2   GLYGLUARGALASERALAGLUGLNLYSGLU
3   SERTYRGLUPROPROLYSPROALAVALGLY
4   PROSERGLYGLUSERVALVALALATHRGLU
5   ALAPHETRPASPASPLEUGLNGLYPHELEU
6   GLUGLNARGLEULYSASPTYRASPGLUALA
7   ASNLYSLEUARGVALLEUPHELYSGLUALA
8   TRPARGSERSERPHE

Samples:

sample_1: Get5, [U-100% 13C; U-100% 15N], 3.5 mM; sodium phosphate 20 mM; sodium azide 0.02%; H2O 90%; D2O 10%

sample_3: Get5, [U-100% 15N], 2.5 mM; sodium phosphate 20 mM; sodium azide 0.02%; polyacrylamide 5%; H2O 90%; D2O 10%

sample_2: Get5, [U-100% 15N], 2.5 mM; sodium phosphate 20 mM; sodium azide 0.02%; H2O 90%; D2O 10%

sample_4: Get5 4 mM; Get5, [U-100% 13C; U-100% 15N], 2 mM; sodium phosphate 20 mM; sodium azide 0.02%; H2O 90%; D2O 10%

sample_conditions_1: ionic strength: 50 mM; pH: 6.1; pressure: 1 atm; temperature: 298 K

Experiments:

NameSampleSample stateSample conditions
2D 1H-15N HSQCsample_1isotropicsample_conditions_1
3D CBCA(CO)NHsample_1isotropicsample_conditions_1
3D C(CO)NHsample_1isotropicsample_conditions_1
3D HNCOsample_1isotropicsample_conditions_1
3D HNCAsample_1isotropicsample_conditions_1
3D HNCACBsample_1isotropicsample_conditions_1
3D HN(CO)CAsample_1isotropicsample_conditions_1
3D HCCH-TOCSYsample_1isotropicsample_conditions_1
2D 1H-13C HSQCsample_1isotropicsample_conditions_1
2D 1H-13C HSQC aromaticsample_1isotropicsample_conditions_1
3D 1H-15N NOESYsample_1isotropicsample_conditions_1
3D 1H-13C NOESYsample_1isotropicsample_conditions_1
3D 1H-15N TOCSYsample_1isotropicsample_conditions_1
3D HBHA(CO)NHsample_1isotropicsample_conditions_1
3D H(CCO)NHsample_1isotropicsample_conditions_1
2D 1H-15N HSQCsample_2isotropicsample_conditions_1
2D 1H-15N HSQCsample_3anisotropicsample_conditions_1
2D 1H-1H NOESYsample_2isotropicsample_conditions_1
2D 1H-13C HSQCsample_4isotropicsample_conditions_1

Software:

VNMRJ, Varian - collection

TOPSPIN, Bruker Biospin - processing

NMRPipe, Delaglio, Grzesiek, Vuister, Zhu, Pfeifer and Bax - processing

Analysis, CCPN - data analysis

TALOS, Cornilescu, Delaglio and Bax - data analysis

PINE, Bahrami, Markley, Assadi, and Eghbalnia - chemical shift assignment

ARIA v2.3, Linge, O'Donoghue and Nilges - structure solution

REDCAT, Prestegard - data analysis

CNS v1.21, Brunger, Adams, Clore, Gros, Nilges and Read - refinement

NMR spectrometers:

  • Varian INOVA 600 MHz

Related Database Links:

PDB
GB EAL86127 EDP51028 KEY82654 KMK58944
REF XP_748165

Download HSQC peak lists in one of the following formats:
CSV: Backbone or all simulated peaks
SPARKY: Backbone or all simulated peaks