BMRB Entry 18176
Chem Shift validation: AVS_full
BMRB Entry DOI: doi:10.13018/BMR18176
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NMR-STAR v3 text file.
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Citation: Christensen, Lea Cecilie; Jensen, Njal Winther; Vala, Andrea; Kamarauskaite, Jurate; Johansson, Linda; Winther, Jakob Rahr; Hofmann, Kay; Teilum, Kaare; Ellgaard, Lars. "The human selenoprotein VCP-interacting membrane protein (VIMP) is non-globular and harbors a reductase function in an intrinsically disordered region." J. Biol. Chem. 287, 26388-26399 (2012).
PubMed: 22700979
Assembly members:
cVIMP-Cys_ox, polymer, 142 residues, Formula weight is not available
Natural source: Common Name: Human Taxonomy ID: 9606 Superkingdom: Eukaryota Kingdom: Metazoa Genus/species: Homo sapiens
Experimental source: Production method: recombinant technology Host organism: Escherichia coli Vector: pLE358
Entity Sequences (FASTA):
cVIMP-Cys_ox: MQKLSARLRALRQRQLDRAA
AAVEPDVVVKRQEALAAARL
KMQEELNAQVEKHKEKLKQL
EEEKRRQKIEMWDSMQEGKS
YKGNAKKPQEEDSPGPSTSS
VLKRKSDRKPLRGGGYNPLS
GEGGGACSWRPGRRGPSSGG
CG
- assigned_chemical_shifts
| Data type | Count |
| 13C chemical shifts | 169 |
| 15N chemical shifts | 81 |
| 1H chemical shifts | 81 |
Additional metadata:
Assembly:
| Entity Assembly ID | Entity Name | Entity ID |
|---|---|---|
| 1 | cVIMP-Cys | 1 |
Entities:
Entity 1, cVIMP-Cys 142 residues - Formula weight is not available
Residues 2-142 in the sequence represent residues 49-189 in human VIMP (SelS)
| 1 | MET | GLN | LYS | LEU | SER | ALA | ARG | LEU | ARG | ALA | ||||
| 2 | LEU | ARG | GLN | ARG | GLN | LEU | ASP | ARG | ALA | ALA | ||||
| 3 | ALA | ALA | VAL | GLU | PRO | ASP | VAL | VAL | VAL | LYS | ||||
| 4 | ARG | GLN | GLU | ALA | LEU | ALA | ALA | ALA | ARG | LEU | ||||
| 5 | LYS | MET | GLN | GLU | GLU | LEU | ASN | ALA | GLN | VAL | ||||
| 6 | GLU | LYS | HIS | LYS | GLU | LYS | LEU | LYS | GLN | LEU | ||||
| 7 | GLU | GLU | GLU | LYS | ARG | ARG | GLN | LYS | ILE | GLU | ||||
| 8 | MET | TRP | ASP | SER | MET | GLN | GLU | GLY | LYS | SER | ||||
| 9 | TYR | LYS | GLY | ASN | ALA | LYS | LYS | PRO | GLN | GLU | ||||
| 10 | GLU | ASP | SER | PRO | GLY | PRO | SER | THR | SER | SER | ||||
| 11 | VAL | LEU | LYS | ARG | LYS | SER | ASP | ARG | LYS | PRO | ||||
| 12 | LEU | ARG | GLY | GLY | GLY | TYR | ASN | PRO | LEU | SER | ||||
| 13 | GLY | GLU | GLY | GLY | GLY | ALA | CYS | SER | TRP | ARG | ||||
| 14 | PRO | GLY | ARG | ARG | GLY | PRO | SER | SER | GLY | GLY | ||||
| 15 | CYS | GLY |
Samples:
cVIMP-Cys_ox_sample: D2O 10%; DSS 1%; sodium phosphate 50 mM; EDTA 1 mM; sodium chloride 150 mM; cVIMP-Cys, [U-100% 13C; U-100% 15N], 291 uM; GSSG 5 mM; sodium azide 1%; H2O 90%
sample_conditions_1: pH: 7; pressure: 1 atm; temperature: 273 K
Experiments:
| Name | Sample | Sample state | Sample conditions |
|---|---|---|---|
| 2D 1H-15N HSQC | cVIMP-Cys_ox_sample | isotropic | sample_conditions_1 |
| 3D HNCO | cVIMP-Cys_ox_sample | isotropic | sample_conditions_1 |
| 3D HNCA | cVIMP-Cys_ox_sample | isotropic | sample_conditions_1 |
| 3D HNN | cVIMP-Cys_ox_sample | isotropic | sample_conditions_1 |
Software:
NMRPipe, Delaglio, Grzesiek, Vuister, Zhu, Pfeifer and Bax - processing
VNMRJ, Varian - collection
CCPN-analysis, CCPN - chemical shift assignment
NMR spectrometers:
- Varian INOVA 800 MHz
- Varian INOVA 750 MHz
Related Database Links:
| BMRB | 18177 |
| GB | AAF67483 AAH05840 AAI07775 AAK15708 AAP85541 |
| REF | NP_001108228 NP_001186934 NP_060915 NP_982298 XP_003281631 |
| SP | Q9BQE4 |
| AlphaFold | Q9BQE4 |
Download HSQC peak lists in one of the following formats:
CSV: Backbone
or all simulated peaks
SPARKY: Backbone
or all simulated peaks