BMRB Entry 18160

Name: Solution NMR structure of the C-terminal extension of two bacterial light, oxygen, voltage (LOV) photoreceptor proteins from Pseudomonas putida
Published: 2013-07-08

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PDB ID: 2yon
Entry in NMR Restraints Grid
Validation report in NRG-CING
Chem Shift validation: AVS_full
BMRB Entry DOI: doi:10.13018/BMR18160
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Nicholas Rego and David Koes
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Bioinformatics (2015) 31 (8): 1322-1324 doi:10.1093/bioinformatics/btu829

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Title:

Solution NMR structure of the C-terminal extension of two bacterial light, oxygen, voltage (LOV) photoreceptor proteins from Pseudomonas putida

Deposition date:

2011-12-23

Original release date:

2013-07-08

Authors:

Rani, Raj; Lecher, Justin; Hartmann, Rudolf; Krauss, Ulrich; Jaeger, Karl-Erich; Willbold, Dieter

Citation:

Citation: Rani, Raj; Jentzsch, Katrin; Lecher, Justin; Hartmann, Rudolf; Willbold, Dieter; Jaeger, Karl-Erich; Krauss, Ulrich. "Conservation of Dark Recovery Kinetic Parameters and Structural Features in the Pseudomonadaceae "Short" Light, Oxygen, Voltage (LOV) Protein Family: Implications for the Design of LOV-Based Optogenetic Tools" Biochemistry 52, 4460-4473 (2013).
PubMed: 23746326

Assembly members:

Assembly members:
AceSB2Pep, polymer, 30 residues, 3420.7601 Da.

Natural source:

Natural source: Common Name: Pseudomonas putida Taxonomy ID: 303 Superkingdom: Bacteria Kingdom: not available Genus/species: Pseudomonas putida

Experimental source:

Experimental source: Production method: chemical synthesis

Entity Sequences (FASTA):

Entity Sequences (FASTA):
AceSB2Pep: XTAQVFAEERVRELEAEVAE LRRQQGQAKH

Data sets:

assigned_chemical_shifts
- assigned_chem_shift_list

Data type	Count
1H chemical shifts	214

Additional metadata:

Assembly
Samples and Experiments
Software
Spectrometers
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Assembly:

Entity Assembly ID	Entity Name	Entity ID
1	AceSB2Pep	1

Entities:

Entity 1, AceSB2Pep 30 residues - 3420.7601 Da.

1	ACE	THR	ALA	GLN	VAL	PHE	ALA	GLU	GLU	ARG
2	VAL	ARG	GLU	LEU	GLU	ALA	GLU	VAL	ALA	GLU
3	LEU	ARG	ARG	GLN	GLN	GLY	GLN	ALA	LYS	HIS

Samples:

PpSB2-Jalpha: AceSB2Pep 1.0 mM; sodium phosphate 100.0 mM; sodium chloride 50.0 mM; 2,2,2-Trifluoroethanol 25.0%; H2O 65.0%; deuterium oxide 10.0%

Standard: ionic strength: 150.0 mM; pH: 6.300; pressure: 1.000 atm; temperature: 298.000 K

Experiments:

Name	Sample	Sample state	Sample conditions
2D 1H-1H NOESY	PpSB2-Jalpha	isotropic	Standard

Software:

ANALYSIS v2.2, CCPN - assignment, data evaluation

NMRPipe v7.3.2012.114.11.33, NIH - conversion, processing

VNMRJ v2.3A, Agilent Technologies (formerly Varian) - data recording, spectrometer operation

NMR spectrometers:

Varian VNMRS 900 MHz

UniProt	Q88JB0
PDB	2YON
GB	AAN68347 AEV23113 KMU97968 KMY35015
REF	NP_744883 WP_010953652 WP_029615462
AlphaFold	Q88JB0

1	ACE	THR	ALA	GLN	VAL	PHE	ALA	GLU	GLU	ARG
2	VAL	ARG	GLU	LEU	GLU	ALA	GLU	VAL	ALA	GLU
3	LEU	ARG	ARG	GLN	GLN	GLY	GLN	ALA	LYS	HIS

1	ACE	THR	ALA	GLN	VAL	PHE	ALA	GLU	GLU	ARG
2	VAL	ARG	GLU	LEU	GLU	ALA	GLU	VAL	ALA	GLU
3	LEU	ARG	ARG	GLN	GLN	GLY	GLN	ALA	LYS	HIS