BMRB Entry 18159

Name: Solution NMR structure of the C-terminal extension of two bacterial light, oxygen, voltage (LOV) photoreceptor proteins from Pseudomonas putida
Published: 2013-07-08

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PDB ID: 2yom
Entry in NMR Restraints Grid
Validation report in NRG-CING
Chem Shift validation: AVS_full
BMRB Entry DOI: doi:10.13018/BMR18159
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Nicholas Rego and David Koes
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Bioinformatics (2015) 31 (8): 1322-1324 doi:10.1093/bioinformatics/btu829

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Title:

Solution NMR structure of the C-terminal extension of two bacterial light, oxygen, voltage (LOV) photoreceptor proteins from Pseudomonas putida

Deposition date:

2011-12-23

Original release date:

2013-07-08

Authors:

Rani, Raj; Lecher, Justin; Hartmann, Rudolf; Jaeger, Karl-Erich; Willbold, Dieter; Krauss, Ulrich

Citation:

Citation: Rani, Raj; Jentzsch, Katrin; Lecher, Justin; Hartmann, Rudolf; Willbold, Dieter; Jaeger, Karl-Erich; Krauss, Ulrich. "Conservation of Dark Recovery Kinetic Parameters and Structural Features in the Pseudomonadaceae "Short" Light, Oxygen, Voltage (LOV) Protein Family: Implications for the Design of LOV-Based Optogenetic Tools" Biochemistry 52, 4460-4473 (2013).
PubMed: 23746326

Assembly members:

Assembly members:
AceSB1JalphaPep, polymer, 24 residues, 2790.1037 Da.

Natural source:

Natural source: Common Name: Pseudomonas putida Taxonomy ID: 303 Superkingdom: Bacteria Kingdom: not available Genus/species: Pseudomonas putida

Experimental source:

Experimental source: Production method: chemical synthesis

Entity Sequences (FASTA):

Entity Sequences (FASTA):
AceSB1JalphaPep: XSRQVELERELAELRARPKP DERA

Data sets:

assigned_chemical_shifts
- assigned_chem_shift_list

Data type	Count
1H chemical shifts	175

Additional metadata:

Assembly
Samples and Experiments
Software
Spectrometers
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Assembly:

Entity Assembly ID	Entity Name	Entity ID
1	AceSB1JalphaPep	1

Entities:

Entity 1, AceSB1JalphaPep 24 residues - 2790.1037 Da.

1

ACE

SER

ARG

GLN

VAL

GLU

LEU

GLU

ARG

GLU

2

LEU

ALA

GLU

LEU

ARG

ALA

ARG

PRO

LYS

PRO

3

ASP

GLU

ARG

ALA

Samples:

PpSB1-Jalpha: AceSB1JalphaPep 1.0 mM; deuteriumoxide 10.0%; sodium phosphate 100.0 mM; sodium chloride 50.0 mM; 2,2,2-Trifluoroethanol 10.0%; H2O 80.0%

Standard: ionic strength: 150.0 mM; pH: 6.300; pressure: 1.000 atm; temperature: 298.000 K

Experiments:

Name	Sample	Sample state	Sample conditions
2D 1H-1H NOESY	PpSB1-Jalpha	isotropic	Standard

Software:

ANALYSIS v2.2, CCPN - assignment, data evaluation

NMRPipe v7.3.2012.114.11.33, NIH - conversion, processing

VNMRJ v2.3A, Agilent Technologies (formerly Varian) - data recording, spectrometer operation

NMR spectrometers:

Varian VNMRS 900 MHz

UniProt	Q88E39
PDB	2YOM 3SW1
GB	AAN70202 ABQ80609 ADR61898 AFK68500 AFO47404
REF	NP_746738 WP_003251655 WP_010955290 WP_014592344 WP_019752532
AlphaFold	Q88E39