BMRB Entry 18157

Name: Backbone 1H, 13C, and 15N Chemical Shift Assignments for the Avirulence homolog-5
Published: 2012-01-04

Chem Shift validation: AVS_full, LACS
BMRB Entry DOI: doi:10.13018/BMR18157

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Nicholas Rego and David Koes
3Dmol.js: molecular visualization with WebGL
Bioinformatics (2015) 31 (8): 1322-1324 doi:10.1093/bioinformatics/btu829

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Title:

Backbone 1H, 13C, and 15N Chemical Shift Assignments for the Avirulence homolog-5

Deposition date:

2011-12-20

Original release date:

2012-01-04

Authors:

Azurmendi, Hugo; Sun, Furong; Capelluto, Daniel

Citation:

Citation: Sun, Furong; Kale, Shiv; Azurmendi, Hugo; Li, Dan; Tyler, Brett; Capelluto, Daniel. "Structural basis for interactions of the Phytophthora sojae RxLR effector Avh5 with phosphatidylinositol 3-phosphate and for host cell entry." Mol. Plant Microbe Interact. 26, 330-344 (2013).
PubMed: 23075041

Assembly members:

Assembly members:
Avh5, polymer, 121 residues, 13874 Da.

Natural source:

Natural source: Common Name: Phytophthora sojae Taxonomy ID: 67593 Superkingdom: Eukaryota Kingdom: not available Genus/species: Phytophthora sojae

Experimental source:

Experimental source: Production method: recombinant technology Host organism: Escherichia coli Vector: pGEX6P-1

Entity Sequences (FASTA):

Entity Sequences (FASTA):
Avh5: GPLGSTRVPDDANLQSVNAP VQTVTRSRRFLRTADTDIVY EPKVHNPGKKQVFIEDKLQK ALTDPKKNKKLYARWYNSGF TVKQVEGGLDQNENRELELT YKNLALGYAKYYQARRSQEA K

Data sets:

assigned_chemical_shifts
- assigned_chem_shift_list_1

Data type	Count
13C chemical shifts	323
15N chemical shifts	103
1H chemical shifts	103

Additional metadata:

Assembly
Samples and Experiments
Software
Spectrometers
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Assembly:

Entity Assembly ID	Entity Name	Entity ID
1	Avh5	1

Entities:

Entity 1, Avh5 121 residues - 13874 Da.

Residues 1-5 represent the residues from the vector

1

GLY

PRO

LEU

GLY

SER

THR

ARG

VAL

PRO

ASP

2

ASP

ALA

ASN

LEU

GLN

SER

VAL

ASN

ALA

PRO

3

VAL

GLN

THR

VAL

THR

ARG

SER

ARG

PHE

4

LEU

ARG

THR

ALA

ASP

THR

ASP

ILE

VAL

TYR

5

GLU

PRO

LYS

VAL

HIS

ASN

PRO

GLY

LYS

6

GLN

VAL

PHE

ILE

GLU

ASP

LYS

LEU

GLN

LYS

7

ALA

LEU

THR

ASP

PRO

LYS

ASN

LYS

8

LEU

TYR

ALA

ARG

TRP

TYR

ASN

SER

GLY

PHE

9

THR

VAL

LYS

GLN

VAL

GLU

GLY

LEU

ASP

10

GLN

ASN

GLU

ASN

ARG

GLU

LEU

GLU

LEU

THR

11

TYR

LYS

ASN

LEU

ALA

LEU

GLY

TYR

ALA

LYS

12

TYR

GLN

ALA

ARG

SER

GLN

GLU

ALA

13

LYS

Samples:

sample_1: Avh5, [U-100% 13C; U-100% 15N], 1 mM

sample_conditions_1: ionic strength: 100 mM; pH: 5.8; pressure: 1 atm; temperature: 298.15 K

Experiments:

Name	Sample	Sample state	Sample conditions
2D 1H-15N HSQC	sample_1	isotropic	sample_conditions_1
3D HNCO	sample_1	isotropic	sample_conditions_1
3D HN(CO)CA	sample_1	isotropic	sample_conditions_1
3D HNCACB	sample_1	isotropic	sample_conditions_1
3D CBCA(CO)NH	sample_1	isotropic	sample_conditions_1

Software:

Topspin v2.1, CCPN, Delaglio, Grzesiek, Vuister, Zhu, Pfeifer and Bax - chemical shift assignment, collection, processing

NMR spectrometers:

Bruker Avance 800 MHz

GB	AEK80452 AEK80453 AEK80454 EGZ14529
REF	XP_009528278