BMRB Entry 18155

Chem Shift validation: AVS_full, LACS
BMRB Entry DOI: doi:10.13018/BMR18155

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Title:
1H, 13C, and 15N chemical shift assignments of the second immunoglobulin domain of the membrane receptor Neurolin from Carassius auratus
Deposition date:
2011-12-20
Original release date:
2012-03-27
Authors:
Moller, Heiko; Kulic, Zarko; Fritz, Guenter; Stuermer, Claudia
Citation:

Citation: Kulic, Zarko; Fritz, Gunter; Moller, Heiko. "(1)H, (13)C, and (15)N resonance assignments of the second immunoglobulin domain of neurolin from Carassius auratus."  Biomol. NMR Assignments 7, 65-67 (2013).
PubMed: 22446848

Assembly members:

Assembly members:
NIg2, polymer, 111 residues, 12198.7 Da.

Natural source:

Natural source:   Common Name: goldfish   Taxonomy ID: 7957   Superkingdom: Eukaryota   Kingdom: Metazoa   Genus/species: Carassius auratus

Experimental source:

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli   Vector: pET15b

Entity Sequences (FASTA):

Entity Sequences (FASTA):
NIg2: KPSAPVIKNNAKELENGKLT QLGECVVENANPPADLIWKK NNQTLVDDGKTIIITSTITK DKITGLSSTSSRLQYTARKE DVESQFTCTAKHVMGPDQVS EPESFPIHYPT

Data sets:
Data typeCount
13C chemical shifts476
15N chemical shifts107
1H chemical shifts739

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1Neurolin Ig21

Entities:

Entity 1, Neurolin Ig2 111 residues - 12198.7 Da.

1   LYSPROSERALAPROVALILELYSASNASN
2   ALALYSGLULEUGLUASNGLYLYSLEUTHR
3   GLNLEUGLYGLUCYSVALVALGLUASNALA
4   ASNPROPROALAASPLEUILETRPLYSLYS
5   ASNASNGLNTHRLEUVALASPASPGLYLYS
6   THRILEILEILETHRSERTHRILETHRLYS
7   ASPLYSILETHRGLYLEUSERSERTHRSER
8   SERARGLEUGLNTYRTHRALAARGLYSGLU
9   ASPVALGLUSERGLNPHETHRCYSTHRALA
10   LYSHISVALMETGLYPROASPGLNVALSER
11   GLUPROGLUSERPHEPROILEHISTYRPRO
12   THR

Samples:

sample_1: NIg2, [U-99% 13C; U-99% 15N], 0.90 mM; sodium phosphate 50 mM; sodium chloride 100 mM; sodium azide 4 mM

sample_2: NIg2, [U-99% 13C; U-99% 15N], 0.65 mM; sodium phosphate 50 mM; sodium chloride 100 mM; sodium azide 4 mM

sample_3: NIg2, [U-99% 15N], 1.54 mM; sodium phosphate 50 mM; sodium chloride 100 mM; sodium azide 4 mM

sample_conditions_1: pH: 7.0; pressure: 1 atm; temperature: 302 K

Experiments:

NameSampleSample stateSample conditions
2D 1H-15N HSQCsample_1isotropicsample_conditions_1
3D HNCACBsample_1isotropicsample_conditions_1
3D CBCA(CO)NHsample_1isotropicsample_conditions_1
3D HNCOsample_1isotropicsample_conditions_1
3D HN(CA)COsample_1isotropicsample_conditions_1
3D 1H-15N TOCSYsample_3isotropicsample_conditions_1
3D 1H-15N NOESYsample_3isotropicsample_conditions_1
3D HNHAsample_3isotropicsample_conditions_1
3D HNHBsample_3isotropicsample_conditions_1
2D 1H-13C HSQCsample_1isotropicsample_conditions_1
2D 1H-13C HSQCsample_2isotropicsample_conditions_1
3D CC(CO)NHsample_1isotropicsample_conditions_1
3D HCC(CO)NHsample_1isotropicsample_conditions_1
3D HCCH-COSYsample_2isotropicsample_conditions_1
3D HCCH-TOCSYsample_2isotropicsample_conditions_1
3D (H)CCH-TOCSYsample_2isotropicsample_conditions_1
2D (HB)CB(CGCDCE)HEsample_2isotropicsample_conditions_1
2D (HB)CB(CGCD)HDsample_2isotropicsample_conditions_1
2D (HB)CB(CGCD)H aromaticsample_2isotropicsample_conditions_1
2D 1H-13C HSQC aromaticsample_1isotropicsample_conditions_1
2D 1H-13C HSQC aromaticsample_2isotropicsample_conditions_1
3D 1H-13C NOESY aromaticsample_1isotropicsample_conditions_1
3D 1H-13C NOESY aromaticsample_2isotropicsample_conditions_1

Software:

CARA v1.8.4, Keller and Wuthrich - chemical shift assignment, data analysis

TOPSPIN v3.0, Bruker Biospin - collection, processing

NMR spectrometers:

  • Bruker Avance III 600 MHz

Related Database Links:

GB AAC38015
SP Q90304
AlphaFold Q90304

Download HSQC peak lists in one of the following formats:
CSV: Backbone or all simulated peaks
SPARKY: Backbone or all simulated peaks