BMRB Entry 18154

Chem Shift validation: AVS_anomalous, AVS_full, SPARTA
BMRB Entry DOI: doi:10.13018/BMR18154

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Title:
apo_YqcA
Deposition date:
2011-12-20
Original release date:
2014-04-22
Authors:
Jin, Changwen; Fu, Wenyu
Citation:

Citation: Ye, Qian; Hu, Yunfei; Jin, Changwen. "(1)H, (13)C and (15)N resonance assignments of the apo and holo states of flavodoxin YqcA from Escherichia coli."  Biomol. NMR Assignments ., .-. (2013).
PubMed: 23749454

Assembly members:

Assembly members:
entity, polymer, 149 residues, 16281.191 Da.

Natural source:

Natural source:   Common Name: E. coli   Taxonomy ID: 562   Superkingdom: Bacteria   Kingdom: not available   Genus/species: Escherichia coli

Experimental source:

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli   Vector: pET21a(+)

Entity Sequences (FASTA):

Entity Sequences (FASTA):
entity: MAEIGIFVGTMYGNSLLVAE EAEAILTAQGHKATVFEDPE LSDWLPYQDKYVLVVTSTTG QGDLPDSIVPLFQGIKDSLG FQPNLRYGVIALGDSSYVNF CNGGKQFDALLQEQSAQRVG EMLLIDASENPEPETESNPW VEQWGTLLS

Data sets:
Data typeCount
13C chemical shifts560
15N chemical shifts137
1H chemical shifts931

Time Domain Data

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1YqcA from Escherichia coli1

Entities:

Entity 1, YqcA from Escherichia coli 149 residues - 16281.191 Da.

1   METALAGLUILEGLYILEPHEVALGLYTHR
2   METTYRGLYASNSERLEULEUVALALAGLU
3   GLUALAGLUALAILELEUTHRALAGLNGLY
4   HISLYSALATHRVALPHEGLUASPPROGLU
5   LEUSERASPTRPLEUPROTYRGLNASPLYS
6   TYRVALLEUVALVALTHRSERTHRTHRGLY
7   GLNGLYASPLEUPROASPSERILEVALPRO
8   LEUPHEGLNGLYILELYSASPSERLEUGLY
9   PHEGLNPROASNLEUARGTYRGLYVALILE
10   ALALEUGLYASPSERSERTYRVALASNPHE
11   CYSASNGLYGLYLYSGLNPHEASPALALEU
12   LEUGLNGLUGLNSERALAGLNARGVALGLY
13   GLUMETLEULEUILEASPALASERGLUASN
14   PROGLUPROGLUTHRGLUSERASNPROTRP
15   VALGLUGLNTRPGLYTHRLEULEUSER

Samples:

sample_1: protein, [U-15N], 1 mM; sodium phosphate 30 mM; DTT 40 mM; H2O 90%; D2O 10%

sample_2: protein, [U-13C; U-15N], 1 mM; sodium phosphate 30 mM; DTT 40 mM; H2O 90%; D2O 10%

sample_conditions_1: ionic strength: 180 mM; pH: 7; pressure: 1 atm; temperature: 298 K

Experiments:

NameSampleSample stateSample conditions
2D 1H-15N HSQCsample_1isotropicsample_conditions_1
3D HNCOsample_2isotropicsample_conditions_1
3D CBCA(CO)NHsample_2isotropicsample_conditions_1
3D HNCAsample_2isotropicsample_conditions_1
3D HNCACBsample_2isotropicsample_conditions_1
3D HCCH-TOCSYsample_2isotropicsample_conditions_1
3D HCCH-COSYsample_2isotropicsample_conditions_1
3D 1H-15N NOESYsample_1isotropicsample_conditions_1
3D 1H-13C NOESYsample_2isotropicsample_conditions_1

Software:

CYANA, Guntert, Mumenthaler and Wuthrich - structure solution

AMBER, Case, Darden, Cheatham, III, Simmerling, Wang, Duke, Luo, ... and Kollm - refinement

NMR spectrometers:

  • Bruker Avance 600 MHz
  • Bruker Avance 800 MHz

Related Database Links:

BMRB 19151 19152 25013 25014
PDB
DBJ BAB37073 BAE76862 BAG78574 BAI27051 BAI32080
EMBL CAP77223 CAQ33114 CAQ99718 CAR04300 CAR09403
GB AAB40440 AAC75832 AAG57904 AAN44291 AAN81803
REF NP_311677 NP_417270 NP_708584 WP_000229279 WP_000807726
SP P65367 P65368 P65369
AlphaFold P65367 P65368 P65369

Download HSQC peak lists in one of the following formats:
CSV: Backbone or all simulated peaks
SPARKY: Backbone or all simulated peaks