BMRB Entry 18151

Name: 1H, 13C, and 15N chemical shift assignments for CdnL from Myxoccoccus xanthus
Published: 2012-03-09

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PDB ID: 2lwj
Entry in NMR Restraints Grid
Validation report in NRG-CING
Chem Shift validation: AVS_anomalous, AVS_full, LACS
BMRB Entry DOI: doi:10.13018/BMR18151
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Bioinformatics (2015) 31 (8): 1322-1324 doi:10.1093/bioinformatics/btu829

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Title:

1H, 13C, and 15N chemical shift assignments for CdnL from Myxoccoccus xanthus

Deposition date:

2011-12-19

Original release date:

2012-03-09

Authors:

Jimenez, M. Angeles; Padmanabhan, S.; Mirassou, Yasmina

Citation:

Citation: Mirassou, Yasmina; Elias-Arnanz, Montserrat; Padmanabhan, S.; Jimenez, M. Angeles. "(1)H, (13)C and (15)N assignments of CdnL, an essential protein in Myxococcus xanthus." Biomol. NMR Assignments 7, 51-55 (2013).
PubMed: 22392343

Assembly members:

Assembly members:
CdnL, polymer, 167 residues, 18982.7 Da.

Natural source:

Natural source: Common Name: Myxococcus xanthus Taxonomy ID: 34 Superkingdom: Bacteria Kingdom: not available Genus/species: Myxoccoccus xanthus

Experimental source:

Experimental source: Production method: recombinant technology Host organism: Escherichia coli Vector: pET15b

Entity Sequences (FASTA):

Entity Sequences (FASTA):
CdnL: GSHMQTSFKTGDKAVYPGQG VGEVMGIEHTEVAGQRQSFY VLRILENGMRIMIPINKVGS VGLREIISEEDVKQVYSILK EKDISVDSTTWNRRYREYME KIKTGSVFEIAEVLRDLYLL KGDKDLSFGERKMLDTARSL LIKELSLAKDCSEDEIESDL KKIFNLA

Data sets:

assigned_chemical_shifts
- assigned_chem_shift_list_1

Data type	Count
13C chemical shifts	539
15N chemical shifts	166
1H chemical shifts	1082

Additional metadata:

Assembly
Samples and Experiments
Software
Spectrometers
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Assembly:

Entity Assembly ID	Entity Name	Entity ID
1	CdnL	1

Entities:

Entity 1, CdnL 167 residues - 18982.7 Da.

GSH is the N-terminal cloning tag

1

GLY

SER

HIS

MET

GLN

THR

SER

PHE

LYS

THR

2

GLY

ASP

LYS

ALA

VAL

TYR

PRO

GLY

GLN

GLY

3

VAL

GLY

GLU

VAL

MET

GLY

ILE

GLU

HIS

THR

4

GLU

VAL

ALA

GLY

GLN

ARG

GLN

SER

PHE

TYR

5

VAL

LEU

ARG

ILE

LEU

GLU

ASN

GLY

MET

ARG

6

ILE

MET

ILE

PRO

ILE

ASN

LYS

VAL

GLY

SER

7

VAL

GLY

LEU

ARG

GLU

ILE

SER

GLU

8

ASP

VAL

LYS

GLN

VAL

TYR

SER

ILE

LEU

LYS

9

GLU

LYS

ASP

ILE

SER

VAL

ASP

SER

THR

10

TRP

ASN

ARG

TYR

ARG

GLU

TYR

MET

GLU

11

LYS

ILE

LYS

THR

GLY

SER

VAL

PHE

GLU

ILE

12

ALA

GLU

VAL

LEU

ARG

ASP

LEU

TYR

LEU

13

LYS

GLY

ASP

LYS

ASP

LEU

SER

PHE

GLY

GLU

14

ARG

LYS

MET

LEU

ASP

THR

ALA

ARG

SER

LEU

15

LEU

ILE

LYS

GLU

LEU

SER

LEU

ALA

LYS

ASP

16

CYS

SER

GLU

ASP

GLU

ILE

GLU

SER

ASP

LEU

17

LYS

ILE

PHE

ASN

LEU

ALA

Samples:

sample_1: CdnL 1 mM; sodium phosphate 50 mM; sodium chloride 100 mM; sodium azide 0.05%; beta-mercaptoethanol 2 mM; DSS 0.5 mM

sample_2: CdnL 1 mM; sodium phosphate 50 mM; sodium chloride 100 mM; sodium azide 0.05%; beta-mercaptoethanol 2 mM; DSS 0.5 mM

sample_3: CdnL, [U-100% 13C; U-100% 15N], 1 mM; sodium phosphate 50 mM; sodium chloride 100 mM; sodium azide 0.05%; beta-mercaptoethanol 2 mM; DSS 0.5 mM

sample_4: CdnL, [U-100% 13C; U-100% 15N], 1 mM; sodium phosphate 50 mM; sodium chloride 100 mM; sodium azide 0.05%; beta-mercaptoethanol 2 mM; DSS 0.5 mM

sample_5: CdnL 0.25 mM; sodium phosphate 50 mM; sodium chloride 100 mM; sodium azide 0.05%; beta-mercaptoethanol 2 mM; DSS 0.1 mM

sample_6: CdnL 0.25 mM; sodium phosphate 50 mM; sodium chloride 100 mM; sodium azide 0.05%; beta-mercaptoethanol 2 mM; DSS 0.1 mM

sample_7: CdnL, [U-100% 13C; U-100% 15N], 0.25 mM; sodium phosphate 50 mM; sodium chloride 100 mM; sodium azide 0.05%; beta-mercaptoethanol 2 mM; DSS 0.5 mM

sample_conditions_1: ionic strength: 0.15 M; pH: 7.0; pressure: 1 atm; temperature: 298 K

Experiments:

Name	Sample	Sample state	Sample conditions
2D 1H-1H COSY	sample_1	isotropic	sample_conditions_1
2D 1H-1H TOCSY	sample_1	isotropic	sample_conditions_1
2D 1H-1H NOESY	sample_1	isotropic	sample_conditions_1
2D 1H-1H COSY	sample_2	isotropic	sample_conditions_1
2D 1H-1H TOCSY	sample_2	isotropic	sample_conditions_1
2D 1H-1H NOESY	sample_2	isotropic	sample_conditions_1
2D 1H-15N HSQC	sample_3	isotropic	sample_conditions_1
3D HNCO	sample_3	isotropic	sample_conditions_1
3D HNCA	sample_3	isotropic	sample_conditions_1
3D CBCA(CO)NH	sample_3	isotropic	sample_conditions_1
2D 1H-13C HSQC aliphatic	sample_4	isotropic	sample_conditions_1
3D HCCH-TOCSY	sample_4	isotropic	sample_conditions_1
3D 1H-13C NOESY aliphatic	sample_4	isotropic	sample_conditions_1
2D 1H-1H COSY	sample_5	isotropic	sample_conditions_1
2D 1H-1H TOCSY	sample_5	isotropic	sample_conditions_1
2D 1H-1H NOESY	sample_5	isotropic	sample_conditions_1
2D 1H-1H COSY	sample_6	isotropic	sample_conditions_1
2D 1H-1H TOCSY	sample_6	isotropic	sample_conditions_1
2D 1H-1H NOESY	sample_6	isotropic	sample_conditions_1
2D 1H-15N HSQC	sample_7	isotropic	sample_conditions_1
2D 1H-13C HSQC aliphatic	sample_7	isotropic	sample_conditions_1

Software:

TOPSPIN, Bruker Biospin - collection, processing

SPARKY, Goddard - chemical shift assignment, peak picking

NMR spectrometers:

Bruker Avance 800 MHz

BMRB	15977
PDB	2LT3 2LWJ
GB	ABF89955 ADO71151 AEI66063 AFE08291 AGC44335
REF	WP_002617724 WP_002625147 WP_002639129 WP_011552697 WP_013939202