BMRB Entry 18133

Name: 1H, 13C, 15N backbone and sidechain of human AK1 from Escherichia coli
Published: 2012-06-21

Chem Shift validation: AVS_full, LACS
BMRB Entry DOI: doi:10.13018/BMR18133

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Nicholas Rego and David Koes
3Dmol.js: molecular visualization with WebGL
Bioinformatics (2015) 31 (8): 1322-1324 doi:10.1093/bioinformatics/btu829

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Title:

1H, 13C, 15N backbone and sidechain of human AK1 from Escherichia coli

Deposition date:

2011-12-12

Original release date:

2012-06-21

Authors:

Fu, Cuiping

Citation:

Citation: Fu, Cuiping; Peng, Yu; Liao, Xinli; Guo, Chenyun; Lin, Donghai. "1H, 13C, 15N backbone and side-chain resonance assignments of the human adenylate kinase 1 in apo form." Biomol. NMR Assignments 7, 155-158 (2013).
PubMed: 22706932

Assembly members:

Assembly members:
AK1, polymer, 199 residues, Formula weight is not available

Natural source:

Natural source: Common Name: Human Taxonomy ID: 9606 Superkingdom: Eukaryota Kingdom: Metazoa Genus/species: Homo sapiens

Experimental source:

Experimental source: Production method: recombinant technology Host organism: Escherichia coli Vector: PGEX-6p-1

Entity Sequences (FASTA):

Entity Sequences (FASTA):
AK1: GPLGSMEEKLKKTKIIFVVG GPGSGKGTQCEKIVQKYGYT HLSTGDLLRSEVSSGSARGK KLSEIMEKGQLVPLETVLDM LRDAMVAKVNTSKGFLIDGY PREVQQGEEFERRIGQPTLL LYVDAGPETMTQRLLKRGET SGRVDDNEETIKKRLETYYK ATEPVIAFYEKRGIVRKVNA EGSVDSVFSQVCTHLDALK

Data sets:

assigned_chemical_shifts
- assigned_chem_shift_list_1

Data type	Count
13C chemical shifts	776
15N chemical shifts	188
1H chemical shifts	1045

Additional metadata:

Assembly
Samples and Experiments
Software
Spectrometers
Hide all

Assembly:

Entity Assembly ID	Entity Name	Entity ID
1	AK1	1

Entities:

Entity 1, AK1 199 residues - Formula weight is not available

1

GLY

PRO

LEU

GLY

SER

MET

GLU

LYS

LEU

2

LYS

THR

LYS

ILE

PHE

VAL

GLY

3

GLY

PRO

GLY

SER

GLY

LYS

GLY

THR

GLN

CYS

4

GLU

LYS

ILE

VAL

GLN

LYS

TYR

GLY

TYR

THR

5

HIS

LEU

SER

THR

GLY

ASP

LEU

ARG

SER

6

GLU

VAL

SER

GLY

SER

ALA

ARG

GLY

LYS

7

LYS

LEU

SER

GLU

ILE

MET

GLU

LYS

GLY

GLN

8

LEU

VAL

PRO

LEU

GLU

THR

VAL

LEU

ASP

MET

9

LEU

ARG

ASP

ALA

MET

VAL

ALA

LYS

VAL

ASN

10

THR

SER

LYS

GLY

PHE

LEU

ILE

ASP

GLY

TYR

11

PRO

ARG

GLU

VAL

GLN

GLY

GLU

PHE

12

GLU

ARG

ILE

GLY

GLN

PRO

THR

LEU

13

LEU

TYR

VAL

ASP

ALA

GLY

PRO

GLU

THR

MET

14

THR

GLN

ARG

LEU

LYS

ARG

GLY

GLU

THR

15

SER

GLY

ARG

VAL

ASP

ASN

GLU

THR

16

ILE

LYS

ARG

LEU

GLU

THR

TYR

LYS

17

ALA

THR

GLU

PRO

VAL

ILE

ALA

PHE

TYR

GLU

18

LYS

ARG

GLY

ILE

VAL

ARG

LYS

VAL

ASN

ALA

19

GLU

GLY

SER

VAL

ASP

SER

VAL

PHE

SER

GLN

20

VAL

CYS

THR

HIS

LEU

ASP

ALA

LEU

LYS

Samples:

sample_1: AK1, [U-100% 13C; U-100% 15N], 1.0 mM; H2O 90%; D2O 10%

sample_conditions_1: ionic strength: 160 mM; pH: 6.21; pressure: 1 atm; temperature: 298 K

Experiments:

Name	Sample	Sample state	Sample conditions
2D 1H-15N HSQC	sample_1	isotropic	sample_conditions_1
3D CBCA(CO)NH	sample_1	isotropic	sample_conditions_1
3D C(CO)NH	sample_1	isotropic	sample_conditions_1
3D HCCH-TOCSY	sample_1	isotropic	sample_conditions_1
3D HN(CO)CA	sample_1	isotropic	sample_conditions_1
3D HNCA	sample_1	isotropic	sample_conditions_1
3D HBHA(CO)NH	sample_1	isotropic	sample_conditions_1
3D 1H-15N TOCSY	sample_1	isotropic	sample_conditions_1
3D HNCO	sample_1	isotropic	sample_conditions_1
3D HNCACB	sample_1	isotropic	sample_conditions_1

Software:

SPARKY, Goddard - chemical shift assignment

NMR spectrometers:

Varian INOVA 600 MHz

PDB	1Z83 2C95
DBJ	BAA78534 BAD97221 BAE88118 BAG36497 BAJ20282
EMBL	CAG46775 CAG46801 CAH89771
GB	AAA51686 AAH01116 AAI00023 AAI14797 AAQ02450
REF	NP_000467 NP_001013600 NP_001124519 NP_001124810 NP_001155354
SP	P00568 P00570
TPG	DAA24273
AlphaFold	P00568 P00570