BMRB

Biological Magnetic Resonance Data Bank


A Repository for Data from NMR Spectroscopy on Proteins, Peptides, Nucleic Acids, and other Biomolecules
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BMRB Entry 18130

Title: 1H, 13C and 15N chemical shift assignments of thioredoxin 2 from Pseudomonas aeruginosa PAO1 in its reduced form   PubMed: 23771858

Deposition date: 2011-12-09 Original release date: 2013-04-15

Authors: Garcin, Edwige; Kreuzer, Corinne; Bornet, Olivier; Guerlesquin, Francoise

Citation: Garcin, Edwige; Bornet, Olivier; Elantak, Latifa; Nouailler, Matthieu; Guerlesquin, Francoise; Sebban-Kreuzer, Corinne. "(1)H, (13)C and (15)N backbone and side-chain chemical shift assignments for reduced unusual thioredoxin Patrx2 of Pseudomonas aeruginosa."  Biomol. NMR Assignments ., .-. (2013).

Assembly members:
Pstrx2, polymer, 114 residues, Formula weight is not available

Natural source:   Common Name: Pseudomonas aeruginosa   Taxonomy ID: 287   Superkingdom: Bacteria   Kingdom: not available   Genus/species: Pseudomonas aeruginosa

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli   Vector: pJF119EH

Entity Sequences (FASTA):
Pstrx2: MKTRYSAEAPARDELDRLAG PTLVEFGTDWCGHCQAAQPL LAEVFSDYPEVGHLKVEDGP GRRLGRSFQVKLWPTFVFLR DGREVARVVRPGSASVLEEA FESLVGEGHHHHHH

Data sets:
Data typeCount
13C chemical shifts476
15N chemical shifts106
1H chemical shifts714

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1Pstrx21

Entities:

Entity 1, Pstrx2 114 residues - Formula weight is not available

1   METLYSTHRARGTYRSERALAGLUALAPRO
2   ALAARGASPGLULEUASPARGLEUALAGLY
3   PROTHRLEUVALGLUPHEGLYTHRASPTRP
4   CYSGLYHISCYSGLNALAALAGLNPROLEU
5   LEUALAGLUVALPHESERASPTYRPROGLU
6   VALGLYHISLEULYSVALGLUASPGLYPRO
7   GLYARGARGLEUGLYARGSERPHEGLNVAL
8   LYSLEUTRPPROTHRPHEVALPHELEUARG
9   ASPGLYARGGLUVALALAARGVALVALARG
10   PROGLYSERALASERVALLEUGLUGLUALA
11   PHEGLUSERLEUVALGLYGLUGLYHISHIS
12   HISHISHISHIS

Samples:

sample_1: Pstrx2, [U-99% 13C; U-99% 15N], 0.8 mM; NaCl 150 mM; KPO4 10 mM

sample_conditions_1: ionic strength: 0.15 M; pH: 7.4; pressure: 1 atm; temperature: 300 K

Experiments:

NameSampleSample stateSample conditions
2D 1H-15N HSQCsample_1isotropicsample_conditions_1
2D 1H-13C HSQCsample_1isotropicsample_conditions_1
2D 1H-1H NOESYsample_1isotropicsample_conditions_1
3D CBCA(CO)NHsample_1isotropicsample_conditions_1
3D HNCOsample_1isotropicsample_conditions_1
3D HBHA(CO)NHsample_1isotropicsample_conditions_1
3D HNCACBsample_1isotropicsample_conditions_1
3D HCCH-TOCSYsample_1isotropicsample_conditions_1
2D CBCACOsample_1isotropicsample_conditions_1
3D HN(CA)COsample_1isotropicsample_conditions_1
3D HBHANHsample_1isotropicsample_conditions_1
2D (HB)CB(CGCD)HDsample_1isotropicsample_conditions_1
2D (HB)CB(CGCDCE)HEsample_1isotropicsample_conditions_1

Software:

CARA, Keller and Wuthrich - chemical shift assignment

NMR spectrometers:

  • Bruker Avance 600 MHz

Related Database Links:

KEGG PA2694
PDB
DBJ BAK90565 BAP22888 BAP50507 BAQ39453 BAR67424
EMBL CAW27137 CCQ84919 CDH70702 CDH76831 CDI91023
GB AAG06082 AAT49977 ABJ11915 AEO74761 AFM64519
REF NP_251384 WP_003090556 WP_003101499 WP_003131054 WP_003162117

Download simulated HSQC data in one of the following formats:
CSV: Backbone or all simulated shifts
SPARKY: Backbone or all simulated shifts