BMRB Entry 18126

Name: Backbone Chemical Shifts of the designed protein Z-L2LBT
Published: 2012-09-10

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PDB ID: 2lr2
Entry in NMR Restraints Grid
Validation report in NRG-CING
Chem Shift validation: AVS_full, LACS
BMRB Entry DOI: doi:10.13018/BMR18126
MolProbity Validation Chart

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Nicholas Rego and David Koes
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Bioinformatics (2015) 31 (8): 1322-1324 doi:10.1093/bioinformatics/btu829

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Title:

Backbone Chemical Shifts of the designed protein Z-L2LBT

Deposition date:

2011-12-08

Original release date:

2012-09-10

Authors:

Barb, Adam; Ho, Tienhuei; Prestegard, James

Citation:

Citation: Barb, Adam; Ho, Tienhuei Grace; Flanagan-Steet, Heather; Prestegard, James. "Lanthanide binding and IgG affinity construct: potential applications in solution NMR, MRI, and luminescence microscopy." Protein Sci. 21, 1456-1466 (2012).
PubMed: 22851279

Assembly members:

Assembly members:
Z-L2LBT, polymer, 88 residues, Formula weight is not available

Natural source:

Natural source: Common Name: not available Taxonomy ID: not available Superkingdom: not available Kingdom: not available Genus/species: not available not available

Experimental source:

Experimental source: Production method: recombinant technology Host organism: Escherichia coli Vector: pET29

Entity Sequences (FASTA):

Entity Sequences (FASTA):
Z-L2LBT: MGSSHHHHHHSSGVDNKFNK EQQNAFYEILHLPNLNEEQR NAFIQSLKDDSYIDTNNDGA YEGDELSGSQSANLLAEAKK LNDAQAPK

Data sets:

assigned_chemical_shifts
- assigned_chem_shift_list_1

Data type	Count
13C chemical shifts	223
15N chemical shifts	73
1H chemical shifts	73

Additional metadata:

Assembly
Samples and Experiments
Software
Spectrometers
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Assembly:

Entity Assembly ID	Entity Name	Entity ID
1	Z-L2LBT	1

Entities:

Entity 1, Z-L2LBT 88 residues - Formula weight is not available

1

MET

GLY

SER

HIS

2

SER

GLY

VAL

ASP

ASN

LYS

PHE

ASN

LYS

3

GLU

GLN

ASN

ALA

PHE

TYR

GLU

ILE

LEU

4

HIS

LEU

PRO

ASN

LEU

ASN

GLU

GLN

ARG

5

ASN

ALA

PHE

ILE

GLN

SER

LEU

LYS

ASP

6

SER

TYR

ILE

ASP

THR

ASN

ASP

GLY

ALA

7

TYR

GLU

GLY

ASP

GLU

LEU

SER

GLY

SER

GLN

8

SER

ALA

ASN

LEU

ALA

GLU

ALA

LYS

9

LEU

ASN

ASP

ALA

GLN

ALA

PRO

LYS

Samples:

sample_1: Z-L2LBT, [U-98% 13C; U-98% 15N], 100 – 800 uM; H2O 90%; D2O, [U-2H], 10%

sample_conditions_1: ionic strength: 0.1 M; pH: 8.0; pressure: 1 atm; temperature: 273 K

Experiments:

Name	Sample	Sample state	Sample conditions
2D 1H-15N HSQC	sample_1	isotropic	sample_conditions_1
3D HNCO	sample_1	isotropic	sample_conditions_1
3D HNCACB	sample_1	isotropic	sample_conditions_1
3D HN(COCA)CB	sample_1	isotropic	sample_conditions_1

Software:

NMRView, Johnson, One Moon Scientific - chemical shift assignment

NMR spectrometers:

Varian VXRS 900 MHz
Varian INOVA 600 MHz