BMRB Entry 18125

Name: Resonance assignments of the 56 kDa chimeric avidin in the biotin-bound and free forms
Published: 2012-03-09

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PDB ID: 2mf6
Entry in NMR Restraints Grid
Validation report in NRG-CING
Chem Shift validation: AVS_full, LACS
BMRB Entry DOI: doi:10.13018/BMR18125
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Nicholas Rego and David Koes
3Dmol.js: molecular visualization with WebGL
Bioinformatics (2015) 31 (8): 1322-1324 doi:10.1093/bioinformatics/btu829

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Title:

Resonance assignments of the 56 kDa chimeric avidin in the biotin-bound and free forms

Deposition date:

2011-12-08

Original release date:

2012-03-09

Authors:

Tossavainen, Helena; Helppolainen, Satu; Maatta, Juha; Pihlajamaa, Tero; Hytonen, Vesa; Kulomaa, Markku; Permi, Perttu

Citation:

Citation: Tossavainen, Helena; Helppolainen, Satu; Maatta, Juha; Pihlajamaa, Tero; Hytonen, Vesa; Kulomaa, Markku; Permi, Perttu. "Resonance assignments of the 56kDa chimeric avidin in the biotin-bound and free forms." Biomol. NMR Assignments 7, 35-38 (2013).
PubMed: 22392339

Assembly members:

Assembly members:
avidin, polymer, 129 residues, Formula weight is not available
BTN, non-polymer, 244.311 Da.

Natural source:

Natural source: Common Name: Chicken Taxonomy ID: 9031 Superkingdom: Eukaryota Kingdom: Metazoa Genus/species: Gallus gallus

Experimental source:

Experimental source: Production method: recombinant technology Host organism: Escherichia coli Vector: pET101D

Entity Sequences (FASTA):

Entity Sequences (FASTA):
avidin: QTVARKCSLTGKWTNDLGSN MTIGAVNSRGEFTGTYITAV ADNPGNITLSPLLGIQHKRA SQPTFGFTVNWKFSESTTVF TGQCFIDRNGKEVLKTMWLL RSSVNDIGDDWKATRVGYNI FTRLRTQKE

Data sets:

assigned_chemical_shifts
- assigned_chem_shift_list_2

Data type	Count
13C chemical shifts	418
15N chemical shifts	120
1H chemical shifts	851

Additional metadata:

Assembly
Samples and Experiments
Software
Spectrometers
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Assembly:

Entity Assembly ID	Entity Name	Entity ID
1	avidin	1
2	BTN	1

Entities:

Entity 1, avidin 129 residues - Formula weight is not available

1

GLN

THR

VAL

ALA

ARG

LYS

CYS

SER

LEU

THR

2

GLY

LYS

TRP

THR

ASN

ASP

LEU

GLY

SER

ASN

3

MET

THR

ILE

GLY

ALA

VAL

ASN

SER

ARG

GLY

4

GLU

PHE

THR

GLY

THR

TYR

ILE

THR

ALA

VAL

5

ALA

ASP

ASN

PRO

GLY

ASN

ILE

THR

LEU

SER

6

PRO

LEU

GLY

ILE

GLN

HIS

LYS

ARG

ALA

7

SER

GLN

PRO

THR

PHE

GLY

PHE

THR

VAL

ASN

8

TRP

LYS

PHE

SER

GLU

SER

THR

VAL

PHE

9

THR

GLY

GLN

CYS

PHE

ILE

ASP

ARG

ASN

GLY

10

LYS

GLU

VAL

LEU

LYS

THR

MET

TRP

LEU

11

ARG

SER

VAL

ASN

ASP

ILE

GLY

ASP

12

TRP

LYS

ALA

THR

ARG

VAL

GLY

TYR

ASN

ILE

13

PHE

THR

ARG

LEU

ARG

THR

GLN

LYS

GLU

Samples:

sample_1: avidin, [U-13C; U-15N], 0.7 – 1.2 mM; d-biotin0.7 – 1.2 mM; H2O 93%; D2O 7%

sample_2: avidin, [U-13C; U-15N], 0.7 – 1.2 mM; H2O 93%; D2O 7%

sample_conditions_1: pH: 6.5; pressure: 1 atm; temperature: 333 K

sample_conditions_2: pH: 6.5; pressure: 1 atm; temperature: 343 K

Experiments:

Name	Sample	Sample state	Sample conditions
2D 1H-15N HSQC	sample_1	isotropic	sample_conditions_1
2D 1H-13C HSQC	sample_1	isotropic	sample_conditions_1
3D HNCACB	sample_1	isotropic	sample_conditions_1
3D CBCA(CO)NH	sample_1	isotropic	sample_conditions_1
3D H(CCO)NH	sample_1	isotropic	sample_conditions_1
3D C(CO)NH	sample_1	isotropic	sample_conditions_1
3D HCCH-COSY	sample_1	isotropic	sample_conditions_1
(HB)CB(CGCD)HD	sample_1	isotropic	sample_conditions_1
HCCmHm	sample_1	isotropic	sample_conditions_1
3D 1H-13C NOESY	sample_1	isotropic	sample_conditions_1
3D 1H-15N NOESY	sample_1	isotropic	sample_conditions_1

Software:

VNMR, Varian - collection, processing

SPARKY, Goddard - data analysis

NMR spectrometers:

Varian Unity INOVA 800 MHz